Tissue-specific oxidative imbalance and mitochondrial dysfunction during Trypanosoma cruzi infection in mice

Jian Jun Wen, Monisha Dhiman, Elbert B. Whorton, Nisha Garg

Research output: Contribution to journalArticle

46 Citations (Scopus)

Abstract

In this study, we examined the tissue specificity of inflammatory and oxidative responses and mitochondrial dysfunction in mice infected by Trypanosoma cruzi. In acute mice, parasite burden and associated inflammatory infiltrate was detected in all tissues (skeletal muscle > heart > stomach > colon). The extent of oxidative damage and mitochondrial decay was in the order of heart > stomach > skeletal muscle > colon. In chronic mice, a low level of parasite burden and inflammation continued in all tissues; however, oxidant overload and mitochondrial inefficiency mainly persisted in the heart tissue (also detectable in stomach). Further, we noted an unvaryingly high degree of oxidative stress, compromised antioxidant status, and decreased mitochondrial respiratory complex activities in peripheral blood of infected mice. A pair-wise log analysis showed a strong positive correlation in the heart-versus-blood (but not other tissues) levels of oxidative stress markers (malonyldialdehyde, glutathione disulfide), antioxidants (superoxide dismutase, MnSOD, catalase), and mitochondrial inhibition of respiratory complexes (CI/CIII) in infected mice. T. cruzi-induced acute inflammatory and oxidative responses are widespread in different muscle tissues. Antioxidant/oxidant status and mitochondrial function are consistently attenuated in the heart, and reflected in the peripheral-blood of T. cruzi-infected mice. Our results provide an impetus to investigate the peripheral-blood oxidative responses in relation to clinical severity of heart disease in chagasic human patients.

Original languageEnglish (US)
Pages (from-to)1201-1209
Number of pages9
JournalMicrobes and Infection
Volume10
Issue number10-11
DOIs
StatePublished - Aug 2008

Fingerprint

Trypanosoma cruzi
Infection
Stomach
Antioxidants
Oxidants
Colon
Parasites
Skeletal Muscle
Oxidative Stress
Organ Specificity
Glutathione Disulfide
Malondialdehyde
Catalase
Superoxide Dismutase
Heart Diseases
Inflammation
Muscles

Keywords

  • Chagas' disease
  • Mitochondrial dysfunction
  • Oxidant/antioxidant status
  • Tissue specificity
  • Trypanosoma cruzi

ASJC Scopus subject areas

  • Microbiology
  • Immunology
  • Infectious Diseases

Cite this

Tissue-specific oxidative imbalance and mitochondrial dysfunction during Trypanosoma cruzi infection in mice. / Wen, Jian Jun; Dhiman, Monisha; Whorton, Elbert B.; Garg, Nisha.

In: Microbes and Infection, Vol. 10, No. 10-11, 08.2008, p. 1201-1209.

Research output: Contribution to journalArticle

Wen, Jian Jun ; Dhiman, Monisha ; Whorton, Elbert B. ; Garg, Nisha. / Tissue-specific oxidative imbalance and mitochondrial dysfunction during Trypanosoma cruzi infection in mice. In: Microbes and Infection. 2008 ; Vol. 10, No. 10-11. pp. 1201-1209.
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