Tissue-specific requirements for a phosphorylation site in the Fushi tarazu homeodomain

Jianli Dong, Henry M. Krause

Research output: Contribution to journalArticle

4 Citations (Scopus)

Abstract

The homeodomain protein Fushi tarazu (Ftz) is required for several embryonic patterning processes including segmentation and neurogenesis. During the stages that these processes are regulated the protein is differentially phosphorylated, suggesting that phosphorylation plays a role in helping the protein to regulate different functions in different tissues. We showed in a recent study that one of the Ftz phosphorylation sites, a protein kinase A-type site in the N-terminal arm of the homeodomain, is required for normal Ftz-dependent segmentation. Here we test whether phosphorylation of this site (Thr-263) is also required in the developing central nervous system (CNS). A well-established role for Ftz in the CNS is for the differentiation of neurons referred to as RP2 neurons. Absence of Ftz expression in these cells causes a failure of certain target genes to be expressed and subsequent defects in RP2 differentiation. In contrast to its effect on segmentation, we find that mutation of Thr-263 to Ala (or Asp) has no effect on these CNS functions. This suggests that the phosphorylation state of this site is irrelevant for Ftz function in the CNS, and that there are tissue-specific differences in the requirements for Ftz phosphorylation.

Original languageEnglish (US)
Pages (from-to)427-431
Number of pages5
JournalDevelopment Genes and Evolution
Volume209
Issue number7
DOIs
StatePublished - 1999
Externally publishedYes

Fingerprint

nervous system
phosphorylation
Phosphorylation
central nervous system
segmentation
Central Nervous System
protein
neurons
homeodomain proteins
Homeodomain Proteins
Neurons
Nerve Tissue
neurogenesis
cAMP-dependent protein kinase
Neurogenesis
defect
mutation
Cyclic AMP-Dependent Protein Kinases
Proteins
proteins

Keywords

  • Drosophila
  • Fushi tarazu
  • Homeodomain
  • Neurogenesis
  • Phosphorylation

ASJC Scopus subject areas

  • Developmental Biology
  • Cell Biology
  • Genetics
  • Ecology, Evolution, Behavior and Systematics

Cite this

Tissue-specific requirements for a phosphorylation site in the Fushi tarazu homeodomain. / Dong, Jianli; Krause, Henry M.

In: Development Genes and Evolution, Vol. 209, No. 7, 1999, p. 427-431.

Research output: Contribution to journalArticle

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