Tissue-specific requirements for a phosphorylation site in the Fushi tarazu homeodomain

Jianli Dong, Henry M. Krause

Research output: Contribution to journalArticle

4 Scopus citations

Abstract

The homeodomain protein Fushi tarazu (Ftz) is required for several embryonic patterning processes including segmentation and neurogenesis. During the stages that these processes are regulated the protein is differentially phosphorylated, suggesting that phosphorylation plays a role in helping the protein to regulate different functions in different tissues. We showed in a recent study that one of the Ftz phosphorylation sites, a protein kinase A-type site in the N-terminal arm of the homeodomain, is required for normal Ftz-dependent segmentation. Here we test whether phosphorylation of this site (Thr-263) is also required in the developing central nervous system (CNS). A well-established role for Ftz in the CNS is for the differentiation of neurons referred to as RP2 neurons. Absence of Ftz expression in these cells causes a failure of certain target genes to be expressed and subsequent defects in RP2 differentiation. In contrast to its effect on segmentation, we find that mutation of Thr-263 to Ala (or Asp) has no effect on these CNS functions. This suggests that the phosphorylation state of this site is irrelevant for Ftz function in the CNS, and that there are tissue-specific differences in the requirements for Ftz phosphorylation.

Original languageEnglish (US)
Pages (from-to)427-431
Number of pages5
JournalDevelopment Genes and Evolution
Volume209
Issue number7
DOIs
StatePublished - Jul 14 1999
Externally publishedYes

    Fingerprint

Keywords

  • Drosophila
  • Fushi tarazu
  • Homeodomain
  • Neurogenesis
  • Phosphorylation

ASJC Scopus subject areas

  • Genetics
  • Developmental Biology

Cite this