TLR5 mediates CD172α + intestinal lamina propria dendritic cell induction of Th17 cells

Han Liu, Feidi Chen, Wei Wu, Anthony T. Cao, Xiaochang Xue, Suxia Yao, Heather L. Evans-Marin, Yan Qing Li, Yingzi Cong

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Abstract

Multiple mechanisms exist in regulation of host responses to massive challenges from microbiota to maintain immune homeostasis in the intestines. Among these is the enriched Th17 cells in the intestines, which regulates intestinal homeostasis through induction of antimicrobial peptides and secretory IgA among others. However, the means by which Th17 cells develop in response to microbiota is still not completely understood. Although both TLR5 and CD172α + lamina propria dendritic cells (LPDC) have been shown to promote Th17 cell development, it is still unclear whether TLR5 mediates the CD172α + LPDC induction of Th17 cells. By using a microbiota antigen-specific T cell reporter mouse system, we demonstrated that microbiota antigen-specific T cells developed into Th17 cells in the intestinal LP, but not in the spleen when transferred into TCRβx-a-/a- mice. LPDCs expressed high levels of TLR5, and most CD172α + LPDCs also co-expressed TLR5. LPDCs produced high levels of IL-23, IL-6 and TGFβ when stimulated with commensal flagellin and promoted Th17 cell development when cultured with full-length CBir1 flagellin but not CBir1 peptide. Wild-type CD172α +, but not CD172α a-, LPDCs induced Th17 cells, whereas TLR5-deficient LPDC did not induce Th17 cells. Our data thereby demonstrated that TLR5 mediates CD172α + LPDC induction of Th17 cells in the intestines.

Original languageEnglish (US)
Article number22040
JournalScientific Reports
Volume6
DOIs
StatePublished - Feb 24 2016

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Th17 Cells
Dendritic Cells
Mucous Membrane
Microbiota
Intestines
Homeostasis
Interleukin-23
T-Lymphocytes
Flagellin
Antigens
Secretory Immunoglobulin A
Peptides
Interleukin-6
Spleen

ASJC Scopus subject areas

  • General

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TLR5 mediates CD172α + intestinal lamina propria dendritic cell induction of Th17 cells. / Liu, Han; Chen, Feidi; Wu, Wei; Cao, Anthony T.; Xue, Xiaochang; Yao, Suxia; Evans-Marin, Heather L.; Li, Yan Qing; Cong, Yingzi.

In: Scientific Reports, Vol. 6, 22040, 24.02.2016.

Research output: Contribution to journalArticle

Liu, Han ; Chen, Feidi ; Wu, Wei ; Cao, Anthony T. ; Xue, Xiaochang ; Yao, Suxia ; Evans-Marin, Heather L. ; Li, Yan Qing ; Cong, Yingzi. / TLR5 mediates CD172α + intestinal lamina propria dendritic cell induction of Th17 cells. In: Scientific Reports. 2016 ; Vol. 6.
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