ToIC and DsbA are needed for the secretion of STB, a heat-stable enterotoxin of Escherichia coli

Devin T. Foreman, Ygnacio Martinez, Gary Coombs, Alfredo Torres, Yankel M. Kupersztoch

Research output: Contribution to journalArticle

41 Citations (Scopus)

Abstract

STB secretion-deficient mutants were isolated using the synthetic transposon TnβlaM. Cultures were plated using a double-membrane system of cellulose acetate and nitrocellulose placed on Luria agar plates containing carbenicillin. The STB bound to the underlying nitrocellulose membrane was detected with anti-STB antibodies. The altered genes of two STB secretion-deficient mutants were identified by conjugation and complementation as tolC and dsbA. In cultures of well-characterized dsbA and tolC mutants, STB was absent from the culture supernatant. The role of TolC and DsbA in the secretion of peptides is discussed.

Original languageEnglish (US)
Pages (from-to)237-245
Number of pages9
JournalMolecular Microbiology
Volume18
Issue number2
StatePublished - Oct 1995
Externally publishedYes

Fingerprint

Collodion
Enterotoxins
Hot Temperature
Escherichia coli
Carbenicillin
Membranes
Agar
Anti-Idiotypic Antibodies
Peptides
Genes
acetylcellulose

ASJC Scopus subject areas

  • Molecular Biology
  • Microbiology

Cite this

Foreman, D. T., Martinez, Y., Coombs, G., Torres, A., & Kupersztoch, Y. M. (1995). ToIC and DsbA are needed for the secretion of STB, a heat-stable enterotoxin of Escherichia coli. Molecular Microbiology, 18(2), 237-245.

ToIC and DsbA are needed for the secretion of STB, a heat-stable enterotoxin of Escherichia coli. / Foreman, Devin T.; Martinez, Ygnacio; Coombs, Gary; Torres, Alfredo; Kupersztoch, Yankel M.

In: Molecular Microbiology, Vol. 18, No. 2, 10.1995, p. 237-245.

Research output: Contribution to journalArticle

Foreman, DT, Martinez, Y, Coombs, G, Torres, A & Kupersztoch, YM 1995, 'ToIC and DsbA are needed for the secretion of STB, a heat-stable enterotoxin of Escherichia coli', Molecular Microbiology, vol. 18, no. 2, pp. 237-245.
Foreman, Devin T. ; Martinez, Ygnacio ; Coombs, Gary ; Torres, Alfredo ; Kupersztoch, Yankel M. / ToIC and DsbA are needed for the secretion of STB, a heat-stable enterotoxin of Escherichia coli. In: Molecular Microbiology. 1995 ; Vol. 18, No. 2. pp. 237-245.
@article{06017875b2584aa28a6edf6edccc5cfa,
title = "ToIC and DsbA are needed for the secretion of STB, a heat-stable enterotoxin of Escherichia coli",
abstract = "STB secretion-deficient mutants were isolated using the synthetic transposon TnβlaM. Cultures were plated using a double-membrane system of cellulose acetate and nitrocellulose placed on Luria agar plates containing carbenicillin. The STB bound to the underlying nitrocellulose membrane was detected with anti-STB antibodies. The altered genes of two STB secretion-deficient mutants were identified by conjugation and complementation as tolC and dsbA. In cultures of well-characterized dsbA and tolC mutants, STB was absent from the culture supernatant. The role of TolC and DsbA in the secretion of peptides is discussed.",
author = "Foreman, {Devin T.} and Ygnacio Martinez and Gary Coombs and Alfredo Torres and Kupersztoch, {Yankel M.}",
year = "1995",
month = "10",
language = "English (US)",
volume = "18",
pages = "237--245",
journal = "Molecular Microbiology",
issn = "0950-382X",
publisher = "Wiley-Blackwell",
number = "2",

}

TY - JOUR

T1 - ToIC and DsbA are needed for the secretion of STB, a heat-stable enterotoxin of Escherichia coli

AU - Foreman, Devin T.

AU - Martinez, Ygnacio

AU - Coombs, Gary

AU - Torres, Alfredo

AU - Kupersztoch, Yankel M.

PY - 1995/10

Y1 - 1995/10

N2 - STB secretion-deficient mutants were isolated using the synthetic transposon TnβlaM. Cultures were plated using a double-membrane system of cellulose acetate and nitrocellulose placed on Luria agar plates containing carbenicillin. The STB bound to the underlying nitrocellulose membrane was detected with anti-STB antibodies. The altered genes of two STB secretion-deficient mutants were identified by conjugation and complementation as tolC and dsbA. In cultures of well-characterized dsbA and tolC mutants, STB was absent from the culture supernatant. The role of TolC and DsbA in the secretion of peptides is discussed.

AB - STB secretion-deficient mutants were isolated using the synthetic transposon TnβlaM. Cultures were plated using a double-membrane system of cellulose acetate and nitrocellulose placed on Luria agar plates containing carbenicillin. The STB bound to the underlying nitrocellulose membrane was detected with anti-STB antibodies. The altered genes of two STB secretion-deficient mutants were identified by conjugation and complementation as tolC and dsbA. In cultures of well-characterized dsbA and tolC mutants, STB was absent from the culture supernatant. The role of TolC and DsbA in the secretion of peptides is discussed.

UR - http://www.scopus.com/inward/record.url?scp=0028787642&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=0028787642&partnerID=8YFLogxK

M3 - Article

VL - 18

SP - 237

EP - 245

JO - Molecular Microbiology

JF - Molecular Microbiology

SN - 0950-382X

IS - 2

ER -