ToIC and DsbA are needed for the secretion of STB, a heat‐stable enterotoxin of Escherichia coli

Devin T. Foreman, Ygnacio Martinez, Gary Coombs, Alfredo Torres, Yankel M. Kupersztoch

Research output: Contribution to journalArticle

42 Scopus citations

Abstract

STB secretion‐deficient mutants were isolated using the synthetic transposon TnβIaM. Cultures were plated using a double‐membrane system of cellulose acetate and nitrocellulose placed on Luria agar plates containing carbenicillin. The STB bound to the underlying nitrocellulose membrane was detected with anti‐STB antibodies. The altered genes of two STB secretion‐deficient mutants were identified by conjugation and complementation as toIC and dsbA. In cultures of well‐characterized dsbA and toIC mutants, STB was absent from the culture supernatant. The role of ToIC and DsbA in the secretion of peptides is discussed.

Original languageEnglish (US)
Pages (from-to)237-245
Number of pages9
JournalMolecular Microbiology
Volume18
Issue number2
DOIs
StatePublished - Oct 1995
Externally publishedYes

ASJC Scopus subject areas

  • Microbiology
  • Molecular Biology

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