Abstract
STB secretion‐deficient mutants were isolated using the synthetic transposon TnβIaM. Cultures were plated using a double‐membrane system of cellulose acetate and nitrocellulose placed on Luria agar plates containing carbenicillin. The STB bound to the underlying nitrocellulose membrane was detected with anti‐STB antibodies. The altered genes of two STB secretion‐deficient mutants were identified by conjugation and complementation as toIC and dsbA. In cultures of well‐characterized dsbA and toIC mutants, STB was absent from the culture supernatant. The role of ToIC and DsbA in the secretion of peptides is discussed.
| Original language | English (US) |
|---|---|
| Pages (from-to) | 237-245 |
| Number of pages | 9 |
| Journal | Molecular Microbiology |
| Volume | 18 |
| Issue number | 2 |
| DOIs | |
| State | Published - Oct 1995 |
| Externally published | Yes |
ASJC Scopus subject areas
- Microbiology
- Molecular Biology
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Foreman, D. T., Martinez, Y., Coombs, G., Torres, A., & Kupersztoch, Y. M. (1995). ToIC and DsbA are needed for the secretion of STB, a heat‐stable enterotoxin of Escherichia coli. Molecular Microbiology, 18(2), 237-245. https://doi.org/10.1111/j.1365-2958.1995.mmi_18020237.x