Toll-like receptor 7-induced immune response to cutaneous West Nile virus infection

Thomas Welte, Krystle Reagan, Hao Fang, Carlos Machain-Williams, Xin Zheng, Nicole Mendell, Gwong Jen J Chang, Ping Wu, Carol D. Blair, Tian Wang

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Abstract

The Toll-like receptor (TLR) 7 response represents a vital host-defence mechanism in a murine model of systemic West Nile virus (WNV) infection. Here, we investigated the role of the TLR7- induced immune response following cutaneous WNV infection. We found that there was no difference in susceptibility to WNV encephalitis between wild-type and TLR7-/ mice upon intradermal injection or infected mosquito feeding. Viral load analysis revealed similar levels of WNV RNA in the peripheral tissues and brains of these two groups of mice following intradermal infection. There was a higher level of cytokines in the blood of wild-type mice at early stages of infection; however, this difference was diminished in the blood and brains at later stages. Langerhans cells (LCs) are permissive to WNV infection and migrate from the skin to draining lymph nodes upon intradermal challenge. Our data showed that WNV infection of TLR7-/- keratinocytes was significantly higher than that of wild-type keratinocytes. Infection of wild-type keratinocytes induced higher levels of alpha interferon and interleukin-1β (IL-1β), IL-6 and IL-12, which might promote LC migration from the skin. Co-culture of naïve LCs of wild-type mice with WNV-infected wild-type keratinocytes resulted in the production of more IL-6 and IL-12 than with TLR7-/- keratinocytes or by cultured LCs alone. Moreover, LCs in the epidermis were reduced in wild-type mice, but not in TLR7-/- mice, following intradermal WNV infection. Overall, our results suggest that the TLR7 response following cutaneous infection promotes LC migration from the skin, which might compromise its protective effect in systemic infection.

Original languageEnglish (US)
Pages (from-to)2660-2668
Number of pages9
JournalJournal of General Virology
Volume90
Issue number11
DOIs
StatePublished - 2009

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Toll-Like Receptor 7
West Nile virus
Virus Diseases
Langerhans Cells
Keratinocytes
Skin
Infection
Interleukin-12
Cell Movement
Interleukin-6
Intradermal Injections
Brain
Encephalitis
Coculture Techniques
Culicidae
Viral Load
Interleukin-1
Interferon-alpha
Epidermis
Cultured Cells

ASJC Scopus subject areas

  • Virology
  • Medicine(all)

Cite this

Welte, T., Reagan, K., Fang, H., Machain-Williams, C., Zheng, X., Mendell, N., ... Wang, T. (2009). Toll-like receptor 7-induced immune response to cutaneous West Nile virus infection. Journal of General Virology, 90(11), 2660-2668. https://doi.org/10.1099/vir.0.011783-0

Toll-like receptor 7-induced immune response to cutaneous West Nile virus infection. / Welte, Thomas; Reagan, Krystle; Fang, Hao; Machain-Williams, Carlos; Zheng, Xin; Mendell, Nicole; Chang, Gwong Jen J; Wu, Ping; Blair, Carol D.; Wang, Tian.

In: Journal of General Virology, Vol. 90, No. 11, 2009, p. 2660-2668.

Research output: Contribution to journalArticle

Welte, T, Reagan, K, Fang, H, Machain-Williams, C, Zheng, X, Mendell, N, Chang, GJJ, Wu, P, Blair, CD & Wang, T 2009, 'Toll-like receptor 7-induced immune response to cutaneous West Nile virus infection', Journal of General Virology, vol. 90, no. 11, pp. 2660-2668. https://doi.org/10.1099/vir.0.011783-0
Welte T, Reagan K, Fang H, Machain-Williams C, Zheng X, Mendell N et al. Toll-like receptor 7-induced immune response to cutaneous West Nile virus infection. Journal of General Virology. 2009;90(11):2660-2668. https://doi.org/10.1099/vir.0.011783-0
Welte, Thomas ; Reagan, Krystle ; Fang, Hao ; Machain-Williams, Carlos ; Zheng, Xin ; Mendell, Nicole ; Chang, Gwong Jen J ; Wu, Ping ; Blair, Carol D. ; Wang, Tian. / Toll-like receptor 7-induced immune response to cutaneous West Nile virus infection. In: Journal of General Virology. 2009 ; Vol. 90, No. 11. pp. 2660-2668.
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