Topical metal chelation therapy ameliorates oxidation-induced toxicity in diabetic cataract

Min Zhang, Mohammad Shoeb, Ping Liu, Tianlin Xiao, Dale Hogan, Ira G. Wong, Gerald A. Campbell, Naseem H. Ansari

Research output: Contribution to journalArticle

16 Scopus citations

Abstract

Oxidative stress plays a critical role in cataractogenesis, the leading cause of blindness worldwide. Since transition metals generate reactive oxygen species (ROS) formation, metal chelation therapy has been proposed for treatment of cataracts. However, the effectiveness of most chelators is limited by low tissue penetrability. This study is the first to demonstrate that the topically applied divalent metal chelator ethylenediamine tetraacetic acid (EDTA) combined with the carrier and permeability enhancer methyl sulfonyl methane (MSM) ameliorates both oxidation-induced lens opacification and the associated toxic accumulation of protein-4-hydroxynonenal (HNE) adducts. Both in vitro (rat lens culture) and in vivo (diabetic rats), EDTA-MSM (1) significantly reduced lens opacification by about 40-50%, (2) significantly diminished lens epithelial cell proliferation and fiber cell swelling in early stages of cataract formation in vivo, and (3) notably decreased the levels of protein-HNE adducts. These findings have important implications specifically for the treatment of cataract and generally for other diseases in which oxidative stress plays a key pathogenic role.

Original languageEnglish (US)
Pages (from-to)380-391
Number of pages12
JournalJournal of Toxicology and Environmental Health - Part A: Current Issues
Volume74
Issue number6
DOIs
StatePublished - Jan 1 2011

ASJC Scopus subject areas

  • Toxicology
  • Health, Toxicology and Mutagenesis

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