Topical Sulfamylon cream inhibits DNA and protein synthesis in the skin donor site wound

Xiao Jun Zhang, John P. Heggers, David L. Chinkes, Steven Wolf, Hal K. Hawkins, Robert R. Wolfe

Research output: Contribution to journalArticle

6 Citations (Scopus)

Abstract

Background: Whereas Sulfamylon is effective in treatment of burn wound infection, controversy exists regarding its effect on the healing process. Methods: A partial thickness skin donor site wound was created on the back and indwelling catheters were placed in the carotid artery and jugular vein in rabbits under general anesthesia. Sulfamylon cream (8.5%, BERTEK Pharmaceuticals Inc., Morgantown, W Va) was applied on the wound, with either open or occlusive dressing. The control wound was covered with dressings only. On day 7 after injury, stable isotope tracers were infused to determine the fractional synthetic rate (FSR) of DNA, and FSR and fractional breakdown rate (FBR) of protein in the wound. Results: In the Sulfamylon-open dressing group, the DNA FSR was 1.3 ± 0.6%/day, the protein FSR was 8.0 ± 3.5%/day, and the net protein deposition (FSR - FBR) was -0.3 ± 3.7%/day. These values were lower (P < .01 to .05) than the corresponding values in the control group (DNA FSR: 2.9 ± 0.9%/day; protein FSR: 20.5 ± 8.4%/day; net protein deposition: 7.9 ± 6.0%/day). Sulfamylon cream selectively inhibited DNA FSR from the de novo base synthesis pathway (2.3 ± 1.2 vs 0.8 ± 0.5%/day, P < .05 vs control). With the occlusive dressing Sulfamylon cream did not decrease wound DNA FSR due to a stimulation of the base salvage pathway, but still decreased protein FSR (11.5 ± 5.1%/day, P < .05 vs control). Histologic slides indicated that Sulfamylon cream inhibited re-epithelialization, collagen formation, and angiogenesis in the wound. Conclusions: Topical Sulfamylon cream application inhibited DNA and protein synthesis in the wound, which would be expected to retard the healing process.

Original languageEnglish (US)
Pages (from-to)633-639
Number of pages7
JournalSurgery
Volume139
Issue number5
DOIs
StatePublished - May 2006

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Mafenide
Skin
DNA
Wounds and Injuries
Proteins
Occlusive Dressings
Bandages
Re-Epithelialization
Indwelling Catheters
Jugular Veins
Wound Infection
Carotid Arteries
Isotopes
General Anesthesia
Collagen
Rabbits

ASJC Scopus subject areas

  • Surgery

Cite this

Zhang, X. J., Heggers, J. P., Chinkes, D. L., Wolf, S., Hawkins, H. K., & Wolfe, R. R. (2006). Topical Sulfamylon cream inhibits DNA and protein synthesis in the skin donor site wound. Surgery, 139(5), 633-639. https://doi.org/10.1016/j.surg.2005.10.013

Topical Sulfamylon cream inhibits DNA and protein synthesis in the skin donor site wound. / Zhang, Xiao Jun; Heggers, John P.; Chinkes, David L.; Wolf, Steven; Hawkins, Hal K.; Wolfe, Robert R.

In: Surgery, Vol. 139, No. 5, 05.2006, p. 633-639.

Research output: Contribution to journalArticle

Zhang, XJ, Heggers, JP, Chinkes, DL, Wolf, S, Hawkins, HK & Wolfe, RR 2006, 'Topical Sulfamylon cream inhibits DNA and protein synthesis in the skin donor site wound', Surgery, vol. 139, no. 5, pp. 633-639. https://doi.org/10.1016/j.surg.2005.10.013
Zhang, Xiao Jun ; Heggers, John P. ; Chinkes, David L. ; Wolf, Steven ; Hawkins, Hal K. ; Wolfe, Robert R. / Topical Sulfamylon cream inhibits DNA and protein synthesis in the skin donor site wound. In: Surgery. 2006 ; Vol. 139, No. 5. pp. 633-639.
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title = "Topical Sulfamylon cream inhibits DNA and protein synthesis in the skin donor site wound",
abstract = "Background: Whereas Sulfamylon is effective in treatment of burn wound infection, controversy exists regarding its effect on the healing process. Methods: A partial thickness skin donor site wound was created on the back and indwelling catheters were placed in the carotid artery and jugular vein in rabbits under general anesthesia. Sulfamylon cream (8.5{\%}, BERTEK Pharmaceuticals Inc., Morgantown, W Va) was applied on the wound, with either open or occlusive dressing. The control wound was covered with dressings only. On day 7 after injury, stable isotope tracers were infused to determine the fractional synthetic rate (FSR) of DNA, and FSR and fractional breakdown rate (FBR) of protein in the wound. Results: In the Sulfamylon-open dressing group, the DNA FSR was 1.3 ± 0.6{\%}/day, the protein FSR was 8.0 ± 3.5{\%}/day, and the net protein deposition (FSR - FBR) was -0.3 ± 3.7{\%}/day. These values were lower (P < .01 to .05) than the corresponding values in the control group (DNA FSR: 2.9 ± 0.9{\%}/day; protein FSR: 20.5 ± 8.4{\%}/day; net protein deposition: 7.9 ± 6.0{\%}/day). Sulfamylon cream selectively inhibited DNA FSR from the de novo base synthesis pathway (2.3 ± 1.2 vs 0.8 ± 0.5{\%}/day, P < .05 vs control). With the occlusive dressing Sulfamylon cream did not decrease wound DNA FSR due to a stimulation of the base salvage pathway, but still decreased protein FSR (11.5 ± 5.1{\%}/day, P < .05 vs control). Histologic slides indicated that Sulfamylon cream inhibited re-epithelialization, collagen formation, and angiogenesis in the wound. Conclusions: Topical Sulfamylon cream application inhibited DNA and protein synthesis in the wound, which would be expected to retard the healing process.",
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T1 - Topical Sulfamylon cream inhibits DNA and protein synthesis in the skin donor site wound

AU - Zhang, Xiao Jun

AU - Heggers, John P.

AU - Chinkes, David L.

AU - Wolf, Steven

AU - Hawkins, Hal K.

AU - Wolfe, Robert R.

PY - 2006/5

Y1 - 2006/5

N2 - Background: Whereas Sulfamylon is effective in treatment of burn wound infection, controversy exists regarding its effect on the healing process. Methods: A partial thickness skin donor site wound was created on the back and indwelling catheters were placed in the carotid artery and jugular vein in rabbits under general anesthesia. Sulfamylon cream (8.5%, BERTEK Pharmaceuticals Inc., Morgantown, W Va) was applied on the wound, with either open or occlusive dressing. The control wound was covered with dressings only. On day 7 after injury, stable isotope tracers were infused to determine the fractional synthetic rate (FSR) of DNA, and FSR and fractional breakdown rate (FBR) of protein in the wound. Results: In the Sulfamylon-open dressing group, the DNA FSR was 1.3 ± 0.6%/day, the protein FSR was 8.0 ± 3.5%/day, and the net protein deposition (FSR - FBR) was -0.3 ± 3.7%/day. These values were lower (P < .01 to .05) than the corresponding values in the control group (DNA FSR: 2.9 ± 0.9%/day; protein FSR: 20.5 ± 8.4%/day; net protein deposition: 7.9 ± 6.0%/day). Sulfamylon cream selectively inhibited DNA FSR from the de novo base synthesis pathway (2.3 ± 1.2 vs 0.8 ± 0.5%/day, P < .05 vs control). With the occlusive dressing Sulfamylon cream did not decrease wound DNA FSR due to a stimulation of the base salvage pathway, but still decreased protein FSR (11.5 ± 5.1%/day, P < .05 vs control). Histologic slides indicated that Sulfamylon cream inhibited re-epithelialization, collagen formation, and angiogenesis in the wound. Conclusions: Topical Sulfamylon cream application inhibited DNA and protein synthesis in the wound, which would be expected to retard the healing process.

AB - Background: Whereas Sulfamylon is effective in treatment of burn wound infection, controversy exists regarding its effect on the healing process. Methods: A partial thickness skin donor site wound was created on the back and indwelling catheters were placed in the carotid artery and jugular vein in rabbits under general anesthesia. Sulfamylon cream (8.5%, BERTEK Pharmaceuticals Inc., Morgantown, W Va) was applied on the wound, with either open or occlusive dressing. The control wound was covered with dressings only. On day 7 after injury, stable isotope tracers were infused to determine the fractional synthetic rate (FSR) of DNA, and FSR and fractional breakdown rate (FBR) of protein in the wound. Results: In the Sulfamylon-open dressing group, the DNA FSR was 1.3 ± 0.6%/day, the protein FSR was 8.0 ± 3.5%/day, and the net protein deposition (FSR - FBR) was -0.3 ± 3.7%/day. These values were lower (P < .01 to .05) than the corresponding values in the control group (DNA FSR: 2.9 ± 0.9%/day; protein FSR: 20.5 ± 8.4%/day; net protein deposition: 7.9 ± 6.0%/day). Sulfamylon cream selectively inhibited DNA FSR from the de novo base synthesis pathway (2.3 ± 1.2 vs 0.8 ± 0.5%/day, P < .05 vs control). With the occlusive dressing Sulfamylon cream did not decrease wound DNA FSR due to a stimulation of the base salvage pathway, but still decreased protein FSR (11.5 ± 5.1%/day, P < .05 vs control). Histologic slides indicated that Sulfamylon cream inhibited re-epithelialization, collagen formation, and angiogenesis in the wound. Conclusions: Topical Sulfamylon cream application inhibited DNA and protein synthesis in the wound, which would be expected to retard the healing process.

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