Topically applied metal chelator reduces thermal injury progression in a rat model of brass comb burn

Cheng Z. Wang, Amina El Ayadi, Juhi Goswamy, Celeste C. Finnerty, Randy Mifflin, Linda Sousse, Perenlei Enkhbaatar, John Papaconstantinou, David N. Herndon, Naseem H. Ansari

Research output: Contribution to journalArticlepeer-review

8 Scopus citations

Abstract

Oxidative stress may be involved in the cellular damage and tissue destruction as burn wounds continues to progress after abatement of the initial insult. Since iron and calcium ions play key roles in oxidative stress, this study tested whether topical application of Livionex formulation (LF) lotion, that contains disodium EDTA as a metal chelator and methyl sulfonyl methane (MSM) as a permeability enhancer, would prevent or reduce burns. Methods We used an established brass comb burn model with some modifications. Topical application of LF lotion was started 5 min post-burn, and repeated every 8 h for 3 consecutive days. Rats were euthanized and skin harvested for histochemistry and immunohistochemistry. Formation of protein adducts of 4-hydroxynonenal (HNE), malonadialdehyde (MDA) and acrolein (ACR) and expression of aldehyde dehydrogenase (ALDH) isozymes, ALDH1 and ALDH2 were assessed. Results LF lotion-treated burn sites and interspaces showed mild morphological improvement compared to untreated burn sites. Furthermore, the lotion significantly decreased the immunostaining of lipid aldehyde-protein adducts including protein -HNE, -MDA and -ACR adducts, and restored the expression of aldehyde dehydrogenase isozymes in the unburned interspaces. Conclusion This data, for the first time, demonstrates that a topically applied EDTA-containing lotion protects burns progression with a concomitant decrease in the accumulation of reactive lipid aldehydes and protection of aldehyde dehydrogenase isozymes. Present studies are suggestive of therapeutic intervention of burns by this novel lotion.

Original languageEnglish (US)
Pages (from-to)1775-1787
Number of pages13
JournalBurns
Volume41
Issue number8
DOIs
StatePublished - 2015

Keywords

  • Brass comb burn
  • Burn progression
  • Iron chelation
  • Oxidative stress
  • Reactive aldehydes
  • Thermal injury
  • Wound healing

ASJC Scopus subject areas

  • Surgery
  • Emergency Medicine
  • Critical Care and Intensive Care Medicine

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