Topographical mapping of GnRH receptors on dispersed mouse pituitary cells by backscattered electron imaging

D. Pal, B. T. Miller, T. A. Parkening

Research output: Contribution to journalArticle

7 Citations (Scopus)

Abstract

Mouse anterior pituitary cells cultured for 2 days were stimulated with one of three biotinylated-GnRH probes ([biotinyl-Lys6]-[D-Lys6]GnRH, [biotinyl-Ser4]-[D-Lys6]GnRH, [biotinyl-Ser4]-[D-Trp6, des-Gly10]GnRH) in the cold (4°C) for 1 hr. These cells were subsequently fixed and an avidin-gold complex was conjugated to the bound GnRH. After a second fixation, the gold label was silver-enhanced for viewing with a scanning electron microscope. Gonadotropes were identified as a result of the labeling procedure, measured for size, and the number of GnRH receptor sites counted. Gonadotropes ranged from 3 to 13 μm in diameter and contained from 23.2 ± 3.3 to 338.4 ± 25.2 sites per cell depending upon the size of the cell and the ligand employed. The methods described should be applicable for studying the topographical distribution of a variety of cellular receptors.

Original languageEnglish (US)
Pages (from-to)89-96
Number of pages8
JournalAnatomical Record
Volume233
Issue number1
DOIs
StatePublished - 1992

Fingerprint

LHRH Receptors
Gonadotropin-Releasing Hormone
electrons
image analysis
Electrons
gold
receptors
mice
avidin
anterior pituitary
Gold
cells
scanning electron microscopes
silver
cultured cells
Avidin
Cell Size
Silver
Cultured Cells
Ligands

ASJC Scopus subject areas

  • Agricultural and Biological Sciences (miscellaneous)
  • Anatomy

Cite this

Topographical mapping of GnRH receptors on dispersed mouse pituitary cells by backscattered electron imaging. / Pal, D.; Miller, B. T.; Parkening, T. A.

In: Anatomical Record, Vol. 233, No. 1, 1992, p. 89-96.

Research output: Contribution to journalArticle

Pal, D. ; Miller, B. T. ; Parkening, T. A. / Topographical mapping of GnRH receptors on dispersed mouse pituitary cells by backscattered electron imaging. In: Anatomical Record. 1992 ; Vol. 233, No. 1. pp. 89-96.
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