Topographical mapping of GnRH receptors on dispersed mouse pituitary cells by backscattered electron imaging

D. Pal, B. T. Miller, T. A. Parkening

Research output: Contribution to journalArticle

7 Scopus citations

Abstract

Mouse anterior pituitary cells cultured for 2 days were stimulated with one of three biotinylated-GnRH probes ([biotinyl-Lys6]-[D-Lys6]GnRH, [biotinyl-Ser4]-[D-Lys6]GnRH, [biotinyl-Ser4]-[D-Trp6, des-Gly10]GnRH) in the cold (4°C) for 1 hr. These cells were subsequently fixed and an avidin-gold complex was conjugated to the bound GnRH. After a second fixation, the gold label was silver-enhanced for viewing with a scanning electron microscope. Gonadotropes were identified as a result of the labeling procedure, measured for size, and the number of GnRH receptor sites counted. Gonadotropes ranged from 3 to 13 μm in diameter and contained from 23.2 ± 3.3 to 338.4 ± 25.2 sites per cell depending upon the size of the cell and the ligand employed. The methods described should be applicable for studying the topographical distribution of a variety of cellular receptors.

Original languageEnglish (US)
Pages (from-to)89-96
Number of pages8
JournalAnatomical Record
Volume233
Issue number1
DOIs
StatePublished - 1992

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ASJC Scopus subject areas

  • Agricultural and Biological Sciences (miscellaneous)
  • Anatomy

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