Topographical mapping of GnRH receptors on dispersed mouse pituitary cells by backscattered electron imaging

Dhananjay Pal, Brian T. Miller, Terry A. Parkening

    Research output: Contribution to journalArticlepeer-review

    7 Scopus citations

    Abstract

    Mouse anterior pituitary cells cultured for 2 days were stimulated with one of three biotinylated‐GnRH probes ([biotinyl‐Lys6]‐[D‐Lys6]GnRH, [biotinyl‐Ser4]‐[D‐Lys6]GnRH, [biotinyl‐Ser4]‐[D‐Trp6, des‐Gly10]GnRH) in the cold (4°C) for 1 hr. These cells were subsequently fixed and an avidin‐gold complex was conjugated to the bound GnRH. After a second fixation, the gold label was silver‐enhanced for viewing with a scanning electron microscope. Gonadotropes were identified as a result of the labeling procedure, measured for size, and the number of GnRH receptor sites counted. Gonadotropes ranged from 3 to 13 μm in diameter and contained from 23.2 ± 3.3 to 338.4 ± 25.2 sites per cell depending upon the size of the cell and the ligand employed. The methods described should be applicable for studying the topographical distribution of a variety of cellular receptors. © 1992 Wiley‐Liss, Inc.

    Original languageEnglish (US)
    Pages (from-to)89-96
    Number of pages8
    JournalThe Anatomical Record
    Volume233
    Issue number1
    DOIs
    StatePublished - May 1992

    ASJC Scopus subject areas

    • Anatomy
    • Agricultural and Biological Sciences (miscellaneous)

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