Topoisomerase III-ß is required for efficient replication of positive-sense RNA viruses

Reddisiva Kambham, Minato Hirano, W. Samuel Fagg, Eileen T. McAnarney, Chao Shan, Xuping Xie, Adam Hage, Colette A. Pietzsch, Alexander Bukreyev, Ricardo Rajsbaum Gorodezky, Pei Yong Shi, Mark T. Bedford, Shelton Bradrick, Vineet Menachery, Mariano A. Garcia-Blanco

Research output: Contribution to journalArticlepeer-review

Abstract

Based on genome-scale loss-of-function screens we discovered that Topoisomerase III-ß (TOP3B), a human topoisomerase that acts on DNA and RNA, is required for yellow fever virus and dengue virus-2 replication. Remarkably, we found that TOP3B is required for efficient replication of all positive-sense-single stranded RNA viruses tested, including SARS-CoV-2. While there are no drugs that specifically inhibit this topoisomerase, we posit that TOP3B is an attractive anti-viral target.

Original languageEnglish (US)
JournalUnknown Journal
DOIs
StatePublished - Mar 27 2020

ASJC Scopus subject areas

  • Biochemistry, Genetics and Molecular Biology(all)
  • Agricultural and Biological Sciences(all)
  • Immunology and Microbiology(all)
  • Neuroscience(all)
  • Pharmacology, Toxicology and Pharmaceutics(all)

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