TY - JOUR
T1 - Topoisomerase III-β is required for efficient replication of positive-sense RNA viruses
AU - Prasanth, K. Reddisiva
AU - Hirano, Minato
AU - Fagg, W. Samuel
AU - McAnarney, Eileen T.
AU - Shan, Chao
AU - Xie, Xuping
AU - Hage, Adam
AU - Pietzsch, Colette A.
AU - Bukreyev, Alexander
AU - Rajsbaum, Ricardo
AU - Shi, Pei Yong
AU - Bedford, Mark T.
AU - Bradrick, Shelton S.
AU - Menachery, Vineet
AU - Garcia-Blanco, Mariano A.
N1 - Funding Information:
We thank members of our laboratories at the University of Texas Medical Branch for comments and suggestions. We also thank Dr. Yuk-Ching Tse Dinh (Florida International University), Dr. Yves Pommier (National Cancer Institute), Dr. Weidong Wang (National Institute on Aging), and Dr. Phillip A. Sharp (MIT) for important discussions and comments. We acknowledge support from the Uehara Foundation Fellowship (MH), NIH R01 CA204806, Vacek Chair, and UTMB startup package (MGB), NIH R01 GM126412 (MTB), NIH AI142759, and the Sealy & Smith, Kleberg, John S. Dunn, Amon G. Carter, Gilson Longenbaugh, and Summerfield Robert Foundations (PYS) , NIH/ NIAID T32 AI007526 ( AH ), R21 AI26012 and R01 AI134907 (RR), UTMB start up funds (VM).
Funding Information:
We thank members of our laboratories at the University of Texas Medical Branch for comments and suggestions. We also thank Dr. Yuk-Ching Tse Dinh (Florida International University), Dr. Yves Pommier (National Cancer Institute), Dr. Weidong Wang (National Institute on Aging), and Dr. Phillip A. Sharp (MIT) for important discussions and comments. We acknowledge support from the Uehara Foundation Fellowship (MH), NIH R01 CA204806, Vacek Chair, and UTMB startup package (MGB), NIH R01 GM126412 (MTB), NIH AI142759, and the Sealy & Smith, Kleberg, John S. Dunn, Amon G. Carter, Gilson Longenbaugh, and Summerfield Robert Foundations (PYS), NIH/NIAID T32 AI007526 (AH), R21 AI26012 and R01 AI134907 (RR), UTMB start up funds (VM).
Publisher Copyright:
© 2020 The Author(s)
PY - 2020/10
Y1 - 2020/10
N2 - Based on genome-scale loss-of-function screens we discovered that Topoisomerase III-β (TOP3B), a human topoisomerase that acts on DNA and RNA, is required for yellow fever virus and dengue virus-2 replication. Remarkably, we found that TOP3B is required for efficient replication of all positive-sense-single stranded RNA viruses tested, including SARS-CoV-2. While there are no drugs that specifically inhibit this topoisomerase, we posit that TOP3B is an attractive anti-viral target.
AB - Based on genome-scale loss-of-function screens we discovered that Topoisomerase III-β (TOP3B), a human topoisomerase that acts on DNA and RNA, is required for yellow fever virus and dengue virus-2 replication. Remarkably, we found that TOP3B is required for efficient replication of all positive-sense-single stranded RNA viruses tested, including SARS-CoV-2. While there are no drugs that specifically inhibit this topoisomerase, we posit that TOP3B is an attractive anti-viral target.
KW - Dengue
KW - Host factor
KW - Positive stranded RNA viruses
KW - SARS-CoV-2
KW - Topoisomerase III-ß (TOP3B)
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U2 - 10.1016/j.antiviral.2020.104874
DO - 10.1016/j.antiviral.2020.104874
M3 - Article
C2 - 32735900
AN - SCOPUS:85089277326
SN - 0166-3542
VL - 182
JO - Antiviral research
JF - Antiviral research
M1 - 104874
ER -