Topoisomerase III-β is required for efficient replication of positive-sense RNA viruses

Reddisiva Kambham, Minato Hirano, W. Samuel Fagg, Eileen T. McAnarney, Chao Shan, Xuping Xie, Adam Hage, Colette A. Pietzsch, Alexander Bukreyev, Ricardo Rajsbaum Gorodezky, Pei Yong Shi, Mark T. Bedford, Shelton Bradrick, Vineet Menachery, Mariano Garcia-Blanco

Research output: Contribution to journalArticle

1 Scopus citations

Abstract

Based on genome-scale loss-of-function screens we discovered that Topoisomerase III-β (TOP3B), a human topoisomerase that acts on DNA and RNA, is required for yellow fever virus and dengue virus-2 replication. Remarkably, we found that TOP3B is required for efficient replication of all positive-sense-single stranded RNA viruses tested, including SARS-CoV-2. While there are no drugs that specifically inhibit this topoisomerase, we posit that TOP3B is an attractive anti-viral target.

Original languageEnglish (US)
Article number104874
JournalAntiviral research
Volume182
DOIs
StatePublished - Oct 2020

Keywords

  • Dengue
  • Host factor
  • Positive stranded RNA viruses
  • SARS-CoV-2
  • Topoisomerase III-ß (TOP3B)

ASJC Scopus subject areas

  • Pharmacology
  • Virology

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