Topoisomerase III-β is required for efficient replication of positive-sense RNA viruses

K. Reddisiva Prasanth; Minato Hirano; W. Samuel Fagg; Eileen T. McAnarney; Chao Shan; Xuping Xie; Adam Hage; Colette A. Pietzsch; Alexander Bukreyev; Ricardo Rajsbaum; Pei Yong Shi; Mark T. Bedford; Shelton S. Bradrick; Vineet Menachery; Mariano A. Garcia-Blanco

doi:10.1016/j.antiviral.2020.104874

Topoisomerase III-β is required for efficient replication of positive-sense RNA viruses

K. Reddisiva Prasanth
, Minato Hirano
, W. Samuel Fagg
, Eileen T. McAnarney
, Chao Shan
, Xuping Xie
, Adam Hage
, Colette A. Pietzsch
, Alexander Bukreyev
, Ricardo Rajsbaum
, Pei Yong Shi
, Mark T. Bedford
, Shelton S. Bradrick
, Vineet Menachery
, Mariano A. Garcia-Blanco

Research output: Contribution to journal › Article › peer-review

20 Scopus citations

Abstract

Based on genome-scale loss-of-function screens we discovered that Topoisomerase III-β (TOP3B), a human topoisomerase that acts on DNA and RNA, is required for yellow fever virus and dengue virus-2 replication. Remarkably, we found that TOP3B is required for efficient replication of all positive-sense-single stranded RNA viruses tested, including SARS-CoV-2. While there are no drugs that specifically inhibit this topoisomerase, we posit that TOP3B is an attractive anti-viral target.

Original language	English (US)
Article number	104874
Journal	Antiviral research
Volume	182
DOIs	https://doi.org/10.1016/j.antiviral.2020.104874
State	Published - Oct 2020

Keywords

Dengue
Host factor
Positive stranded RNA viruses
SARS-CoV-2
Topoisomerase III-ß (TOP3B)

ASJC Scopus subject areas

Pharmacology
Virology

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10.1016/j.antiviral.2020.104874

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Prasanth, K. R., Hirano, M., Fagg, W. S., McAnarney, E. T., Shan, C., Xie, X., Hage, A., Pietzsch, C. A., Bukreyev, A., Rajsbaum, R., Shi, P. Y., Bedford, M. T., Bradrick, S. S., Menachery, V., & Garcia-Blanco, M. A. (2020). Topoisomerase III-β is required for efficient replication of positive-sense RNA viruses. Antiviral research, 182, Article 104874. https://doi.org/10.1016/j.antiviral.2020.104874

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title = "Topoisomerase III-β is required for efficient replication of positive-sense RNA viruses",

abstract = "Based on genome-scale loss-of-function screens we discovered that Topoisomerase III-β (TOP3B), a human topoisomerase that acts on DNA and RNA, is required for yellow fever virus and dengue virus-2 replication. Remarkably, we found that TOP3B is required for efficient replication of all positive-sense-single stranded RNA viruses tested, including SARS-CoV-2. While there are no drugs that specifically inhibit this topoisomerase, we posit that TOP3B is an attractive anti-viral target.",

keywords = "Dengue, Host factor, Positive stranded RNA viruses, SARS-CoV-2, Topoisomerase III-{\ss} (TOP3B)",

author = "Prasanth, \{K. Reddisiva\} and Minato Hirano and Fagg, \{W. Samuel\} and McAnarney, \{Eileen T.\} and Chao Shan and Xuping Xie and Adam Hage and Pietzsch, \{Colette A.\} and Alexander Bukreyev and Ricardo Rajsbaum and Shi, \{Pei Yong\} and Bedford, \{Mark T.\} and Bradrick, \{Shelton S.\} and Vineet Menachery and Garcia-Blanco, \{Mariano A.\}",

note = "Publisher Copyright: {\textcopyright} 2020 The Author(s)",

year = "2020",

month = oct,

doi = "10.1016/j.antiviral.2020.104874",

language = "English (US)",

volume = "182",

journal = "Antiviral research",

issn = "0166-3542",

publisher = "Elsevier B.V.",

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T1 - Topoisomerase III-β is required for efficient replication of positive-sense RNA viruses

AU - Prasanth, K. Reddisiva

AU - Hirano, Minato

AU - Fagg, W. Samuel

AU - McAnarney, Eileen T.

AU - Shan, Chao

AU - Xie, Xuping

AU - Hage, Adam

AU - Pietzsch, Colette A.

AU - Bukreyev, Alexander

AU - Rajsbaum, Ricardo

AU - Shi, Pei Yong

AU - Bedford, Mark T.

AU - Bradrick, Shelton S.

AU - Menachery, Vineet

AU - Garcia-Blanco, Mariano A.

PY - 2020/10

Y1 - 2020/10

N2 - Based on genome-scale loss-of-function screens we discovered that Topoisomerase III-β (TOP3B), a human topoisomerase that acts on DNA and RNA, is required for yellow fever virus and dengue virus-2 replication. Remarkably, we found that TOP3B is required for efficient replication of all positive-sense-single stranded RNA viruses tested, including SARS-CoV-2. While there are no drugs that specifically inhibit this topoisomerase, we posit that TOP3B is an attractive anti-viral target.

AB - Based on genome-scale loss-of-function screens we discovered that Topoisomerase III-β (TOP3B), a human topoisomerase that acts on DNA and RNA, is required for yellow fever virus and dengue virus-2 replication. Remarkably, we found that TOP3B is required for efficient replication of all positive-sense-single stranded RNA viruses tested, including SARS-CoV-2. While there are no drugs that specifically inhibit this topoisomerase, we posit that TOP3B is an attractive anti-viral target.

KW - Dengue

KW - Host factor

KW - Positive stranded RNA viruses

KW - SARS-CoV-2

KW - Topoisomerase III-ß (TOP3B)

UR - https://www.scopus.com/pages/publications/85089277326

UR - https://www.scopus.com/pages/publications/85089277326#tab=citedBy

U2 - 10.1016/j.antiviral.2020.104874

DO - 10.1016/j.antiviral.2020.104874

M3 - Article

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AN - SCOPUS:85089277326

SN - 0166-3542

VL - 182

JO - Antiviral research

JF - Antiviral research

M1 - 104874

ER -

Topoisomerase III-β is required for efficient replication of positive-sense RNA viruses

Abstract

Keywords

ASJC Scopus subject areas

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