Total aminoacyl-transfer RNA pool is greater in liver than muscle in rabbits

Robert R. Wolfe, Juquan Song, Jinchun Sun, Xiao Jun Zhang

Research output: Contribution to journalArticle

10 Citations (Scopus)

Abstract

Transfer RNA (tRNA)-charged amino acids are direct precursors of protein synthesis. Therefore, the amount and profile of amino acids in the aminoacyl-tRNA pool may be closely related to the rate of protein synthesis in the tissue. This study was designed to compare the aminoacyl-tRNA pools in liver and muscle, 2 distinct tissues with different rates of protein synthesis. Liver and muscle samples were taken from 6 rabbits and aminoacyl-tRNA was isolated with sequential acidphenol: chloroform extraction, followed by total RNA and tRNA purification. Amino acids in the aminoacyl-tRNA pool were measured by HPLC after deacylation. Liver contained 3.4 times more tRNA than muscle (585 ± 120 vs. 132 ± 11 μg of tRNA/g of tissue; P < 0.001). Overall tRNA charging was also greater in liver (14.22 ± 4.42 nmol of amino acids/mg of tRNA) than in muscle (7.00 ± 1.76 nmol of amino acids/mg of tRNA) (P < 0.05). The greater availability and charging efficiency of tRNA in liver as compared with muscle may influence the extent to which amino acid precursor availability regulates protein synthesis in these 2 tissues.

Original languageEnglish (US)
Pages (from-to)2333-2338
Number of pages6
JournalJournal of Nutrition
Volume137
Issue number11
StatePublished - Nov 1 2007

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Transfer RNA
Rabbits
Muscles
Liver
Amino Acids
Transfer RNA Aminoacylation
Proteins
Protein Precursors
Chloroform
High Pressure Liquid Chromatography
RNA

ASJC Scopus subject areas

  • Medicine (miscellaneous)
  • Nutrition and Dietetics

Cite this

Total aminoacyl-transfer RNA pool is greater in liver than muscle in rabbits. / Wolfe, Robert R.; Song, Juquan; Sun, Jinchun; Zhang, Xiao Jun.

In: Journal of Nutrition, Vol. 137, No. 11, 01.11.2007, p. 2333-2338.

Research output: Contribution to journalArticle

Wolfe, RR, Song, J, Sun, J & Zhang, XJ 2007, 'Total aminoacyl-transfer RNA pool is greater in liver than muscle in rabbits', Journal of Nutrition, vol. 137, no. 11, pp. 2333-2338.
Wolfe, Robert R. ; Song, Juquan ; Sun, Jinchun ; Zhang, Xiao Jun. / Total aminoacyl-transfer RNA pool is greater in liver than muscle in rabbits. In: Journal of Nutrition. 2007 ; Vol. 137, No. 11. pp. 2333-2338.
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