Total parenteral nutrition-associated alterations in hepatobiliary function and histology in rats: Is light exposure a clue?

J. Bhatia, M. T. Moslen, A. K. Haque, R. McCleery, D. K. Rassin

Research output: Contribution to journalArticle

28 Citations (Scopus)

Abstract

Light exposure of amino acids, especially in the presence of photosensitizers such as riboflavin, has been shown to result in photoproducts that are toxic both in vivo and in vitro. Provision of photooxidized single amino acids has been shown to result in hepatic dysfunction in both gerbils and rats. However, studies of the effects of light exposure of complete nutrient mixtures (glucose-amino acid-vitamins) on hepatobiliary responses are limited. These are important because, in clinical practice, total parenteral nutrition (TPN) solutions are continually exposed to light and because hepatic dysfunction is the most common metabolic aberration associated with TPN. Studies were conducted to compare the effects of TPN that had been exposed to light (+L) or protected from light (-L) on hepatobiliary function of rats. TPN solutions were either exposed to light or protected from light for 24 h and then infused into rats for 10 d. Data for enterally fed rats are also shown for comparison with parenterally fed animals. +L animals lost more weight and had lower bile flow, higher taurocholate output in bile, higher biliary osmolarity, and higher inorganic phosphate in bile. Hepatic histology demonstrated scattered foci of necrosis in eight of the eight +L animals and in only one of eight -L animals. These data demonstrate that protection of TPN solutions from light minimizes TPN- associated alterations in hepatobiliary function and histology. These histologic changes observed in the +L animals are in contrast to previous reports of TPN-induced histologic changes, suggesting a different mechanism. Our observations suggest that consideration be given to the implications of exposing TPN solutions to light.

Original languageEnglish (US)
Pages (from-to)487-492
Number of pages6
JournalPediatric Research
Volume33
Issue number5 I
StatePublished - 1993

Fingerprint

Total Parenteral Nutrition
Histology
Parenteral Nutrition Solutions
Light
Bile
Amino Acids
Liver
Computer Security
Taurocholic Acid
Photosensitizing Agents
Riboflavin
Gerbillinae
Poisons
Vitamins
Osmolar Concentration
Necrosis
Phosphates
Weights and Measures
Glucose
Food

ASJC Scopus subject areas

  • Pediatrics, Perinatology, and Child Health

Cite this

Bhatia, J., Moslen, M. T., Haque, A. K., McCleery, R., & Rassin, D. K. (1993). Total parenteral nutrition-associated alterations in hepatobiliary function and histology in rats: Is light exposure a clue? Pediatric Research, 33(5 I), 487-492.

Total parenteral nutrition-associated alterations in hepatobiliary function and histology in rats : Is light exposure a clue? / Bhatia, J.; Moslen, M. T.; Haque, A. K.; McCleery, R.; Rassin, D. K.

In: Pediatric Research, Vol. 33, No. 5 I, 1993, p. 487-492.

Research output: Contribution to journalArticle

Bhatia, J, Moslen, MT, Haque, AK, McCleery, R & Rassin, DK 1993, 'Total parenteral nutrition-associated alterations in hepatobiliary function and histology in rats: Is light exposure a clue?', Pediatric Research, vol. 33, no. 5 I, pp. 487-492.
Bhatia, J. ; Moslen, M. T. ; Haque, A. K. ; McCleery, R. ; Rassin, D. K. / Total parenteral nutrition-associated alterations in hepatobiliary function and histology in rats : Is light exposure a clue?. In: Pediatric Research. 1993 ; Vol. 33, No. 5 I. pp. 487-492.
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