Toward a global analysis of the human pituitary proteome by multiple gel-based technology

Yingxin Zhao, Francesco Giorgianni, Dominic M. Desiderio, Bing Fang, Sarka Beranova-Giorgianni

Research output: Contribution to journalArticle

24 Scopus citations

Abstract

We describe a comprehensive method for the analysis of complex proteomes, multiple gel-based technology (MGT), which combines in-gel IEF-LC-MS/MS and SDS-PAGE-LC-MS/MS strategies. MGT was applied to the analysis of the proteome of human pituitary tissue. A total of 1449 proteins were uniquely identified. To our knowledge, this is the most comprehensive proteomic analysis of human pituitary tissue to date. Categorization of the identified proteins revealed that MGT provides an excellent and largely unbiased access to proteins with diverse characteristics, including low-abundance proteins, membrane proteins, and proteins with extremes in pI and MW.

Original languageEnglish (US)
Pages (from-to)5324-5331
Number of pages8
JournalAnalytical Chemistry
Volume77
Issue number16
DOIs
StatePublished - Aug 15 2005
Externally publishedYes

ASJC Scopus subject areas

  • Analytical Chemistry

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    Zhao, Y., Giorgianni, F., Desiderio, D. M., Fang, B., & Beranova-Giorgianni, S. (2005). Toward a global analysis of the human pituitary proteome by multiple gel-based technology. Analytical Chemistry, 77(16), 5324-5331. https://doi.org/10.1021/ac050354e