Toward a modular analysis of cell mechanosensing and mechanotransduction: A manual for cell mechanics

Benjamin J. Dubin-Thaler, Michael Sheetz

Research output: Chapter in Book/Report/Conference proceedingChapter

Abstract

Introduction: Cellular mechanosensing and -transduction are critical functions in the shaping of cells and tissues. Although an increasing literature details the proteins and complexes involved in mechanotransduction, how these mechanisms generate the mechanochemical functions of cell motility is often poorly understood. This is a result of the fact that cells can exhibit a number of different types of motility depending on factors such as cell type and local chemical and mechanical perturbations. Due to these factors, even a genetically homogeneous cell population presents a confusing array of different motility phenotypes to the experimentalist. Therefore, we suggest a new approach to understanding cell mechanical functions through reverse systems engineering. Through quantitative analysis, we have observed that, though motility over a population of cells is heterogeneous, at a particular time and location at the cell edge, a cell exhibits only one of a limited number of modular, morphodynamic states of the acto-myosin cytoskeleton. Furthermore, a single motility module can exhibit a heterogeneous cycle of individual steps, with chemical and mechanical interactions changing over the course of this cycle. Thus, much in the way an engineer would describe the functions of components in a car engine, we should be able to approach many problems in cell motility by first describing the molecular steps involved in the basic motility modules and then showing how signaling pathways regulate those modules in order to perform cell-wide functions. In the case of cell motility, we believe there are less than thirty distinct motility modules. With a detailed, quantitative understanding of normal cell motility functions, it will be possible to understand how their malfunction can result in disease processes and to develop therapies that target specific motility modules.

Original languageEnglish (US)
Title of host publicationCellular Mechanotransduction
Subtitle of host publicationDiverse Perspectives from Molecules to Tissues
PublisherCambridge University Press
Pages181-195
Number of pages15
Volume9780521895231
ISBN (Electronic)9781139195874
ISBN (Print)9780521895231
DOIs
StatePublished - Jan 1 2013
Externally publishedYes

Fingerprint

Mechanics
Cells
Reverse engineering
Systems engineering
Railroad cars
Tissue
Engines
Proteins
Engineers
Chemical analysis

ASJC Scopus subject areas

  • Engineering(all)

Cite this

Dubin-Thaler, B. J., & Sheetz, M. (2013). Toward a modular analysis of cell mechanosensing and mechanotransduction: A manual for cell mechanics. In Cellular Mechanotransduction: Diverse Perspectives from Molecules to Tissues (Vol. 9780521895231, pp. 181-195). Cambridge University Press. https://doi.org/10.1017/CBO9781139195874.008

Toward a modular analysis of cell mechanosensing and mechanotransduction : A manual for cell mechanics. / Dubin-Thaler, Benjamin J.; Sheetz, Michael.

Cellular Mechanotransduction: Diverse Perspectives from Molecules to Tissues. Vol. 9780521895231 Cambridge University Press, 2013. p. 181-195.

Research output: Chapter in Book/Report/Conference proceedingChapter

Dubin-Thaler, BJ & Sheetz, M 2013, Toward a modular analysis of cell mechanosensing and mechanotransduction: A manual for cell mechanics. in Cellular Mechanotransduction: Diverse Perspectives from Molecules to Tissues. vol. 9780521895231, Cambridge University Press, pp. 181-195. https://doi.org/10.1017/CBO9781139195874.008
Dubin-Thaler BJ, Sheetz M. Toward a modular analysis of cell mechanosensing and mechanotransduction: A manual for cell mechanics. In Cellular Mechanotransduction: Diverse Perspectives from Molecules to Tissues. Vol. 9780521895231. Cambridge University Press. 2013. p. 181-195 https://doi.org/10.1017/CBO9781139195874.008
Dubin-Thaler, Benjamin J. ; Sheetz, Michael. / Toward a modular analysis of cell mechanosensing and mechanotransduction : A manual for cell mechanics. Cellular Mechanotransduction: Diverse Perspectives from Molecules to Tissues. Vol. 9780521895231 Cambridge University Press, 2013. pp. 181-195
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