Toward the glycome

Structural characterization of gel-separated glycoproteins by tandem high resolution mass spectrometry

Kristina Håkansson, Mark Emmett, Alan G. Marshall, Pia Davidsson, Carol L. Nilsson

Research output: Chapter in Book/Report/Conference proceedingConference contribution

Abstract

Gel-separated glycoproteins were structurally characterized using tandem high resolution mass spectrometry. Proteins were separated by two dimensional PAGE and stained with Coomassie blue or with glycoprotein stain. Spots containing glycoproteins were cut out and digested in-gel with trypsin. The combination of a rapid two dimensional mini-gel glycoprotein separation, glycoprotein visualization, in-gel digestion, and mass spectrometric analysis in a high sensitivity high-resolution instrument allowed the rapid identification of glycoproteins.

Original languageEnglish (US)
Title of host publicationProceedings 50th ASMS Conference on Mass Spectrometry and Allied Topics
Pages83-84
Number of pages2
StatePublished - 2002
Externally publishedYes
EventProceedings - 50th ASMS Conference on Mass Spectrometry and Allied Topics - Orlando, FL, United States
Duration: Jun 2 2002Jun 6 2002

Other

OtherProceedings - 50th ASMS Conference on Mass Spectrometry and Allied Topics
CountryUnited States
CityOrlando, FL
Period6/2/026/6/02

Fingerprint

Mass spectrometry
Glycoproteins
Gels
Trypsin
Coloring Agents
Visualization
Proteins

ASJC Scopus subject areas

  • Spectroscopy

Cite this

Håkansson, K., Emmett, M., Marshall, A. G., Davidsson, P., & Nilsson, C. L. (2002). Toward the glycome: Structural characterization of gel-separated glycoproteins by tandem high resolution mass spectrometry. In Proceedings 50th ASMS Conference on Mass Spectrometry and Allied Topics (pp. 83-84)

Toward the glycome : Structural characterization of gel-separated glycoproteins by tandem high resolution mass spectrometry. / Håkansson, Kristina; Emmett, Mark; Marshall, Alan G.; Davidsson, Pia; Nilsson, Carol L.

Proceedings 50th ASMS Conference on Mass Spectrometry and Allied Topics. 2002. p. 83-84.

Research output: Chapter in Book/Report/Conference proceedingConference contribution

Håkansson, K, Emmett, M, Marshall, AG, Davidsson, P & Nilsson, CL 2002, Toward the glycome: Structural characterization of gel-separated glycoproteins by tandem high resolution mass spectrometry. in Proceedings 50th ASMS Conference on Mass Spectrometry and Allied Topics. pp. 83-84, Proceedings - 50th ASMS Conference on Mass Spectrometry and Allied Topics, Orlando, FL, United States, 6/2/02.
Håkansson K, Emmett M, Marshall AG, Davidsson P, Nilsson CL. Toward the glycome: Structural characterization of gel-separated glycoproteins by tandem high resolution mass spectrometry. In Proceedings 50th ASMS Conference on Mass Spectrometry and Allied Topics. 2002. p. 83-84
Håkansson, Kristina ; Emmett, Mark ; Marshall, Alan G. ; Davidsson, Pia ; Nilsson, Carol L. / Toward the glycome : Structural characterization of gel-separated glycoproteins by tandem high resolution mass spectrometry. Proceedings 50th ASMS Conference on Mass Spectrometry and Allied Topics. 2002. pp. 83-84
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