Towards a mechanism of function of the viral ion channel Vpu from HIV-1

T. Mehnert, Y. H. Lam, P. J. Judge, Andrew Routh, D. Fischer, A. Watts, W. B. Fischer

Research output: Contribution to journalArticle

24 Citations (Scopus)

Abstract

Vpu, an integral membrane protein encoded in HIV-1, is implicated in the release of new virus particles from infected cells, presumably mediated by ion channel activity of homooligomeric Vpu bundles. Reconstitution of both full length Vpu1-81 and a short, the transmembrane (TM) domain comprising peptide Vpu1-32 into bilayers under a constant electric field results in an asymmetric orientation of those channels. For both cases, channel activity with similar kinetics is observed. Channels can open and remain open within a broad series of conductance states even if a small or no electric potential is applied. The mean open time for Vpu peptide channels is voltage-independent. The rate of channel opening shows a biphasic voltage activation, implicating that the gating is influenced by the interaction of the dipole moments of the TM helices with an electric field.

Original languageEnglish (US)
Pages (from-to)589-596
Number of pages8
JournalJournal of Biomolecular Structure and Dynamics
Volume24
Issue number6
StatePublished - Jun 2007
Externally publishedYes

Fingerprint

Ion Channels
Virion
HIV-1
Membrane Proteins
Peptides
peptide 32

Keywords

  • And Gating
  • HIV-1
  • Ion channels
  • Membrane proteins
  • Vpu

ASJC Scopus subject areas

  • Molecular Biology
  • Structural Biology

Cite this

Mehnert, T., Lam, Y. H., Judge, P. J., Routh, A., Fischer, D., Watts, A., & Fischer, W. B. (2007). Towards a mechanism of function of the viral ion channel Vpu from HIV-1. Journal of Biomolecular Structure and Dynamics, 24(6), 589-596.

Towards a mechanism of function of the viral ion channel Vpu from HIV-1. / Mehnert, T.; Lam, Y. H.; Judge, P. J.; Routh, Andrew; Fischer, D.; Watts, A.; Fischer, W. B.

In: Journal of Biomolecular Structure and Dynamics, Vol. 24, No. 6, 06.2007, p. 589-596.

Research output: Contribution to journalArticle

Mehnert, T, Lam, YH, Judge, PJ, Routh, A, Fischer, D, Watts, A & Fischer, WB 2007, 'Towards a mechanism of function of the viral ion channel Vpu from HIV-1', Journal of Biomolecular Structure and Dynamics, vol. 24, no. 6, pp. 589-596.
Mehnert, T. ; Lam, Y. H. ; Judge, P. J. ; Routh, Andrew ; Fischer, D. ; Watts, A. ; Fischer, W. B. / Towards a mechanism of function of the viral ion channel Vpu from HIV-1. In: Journal of Biomolecular Structure and Dynamics. 2007 ; Vol. 24, No. 6. pp. 589-596.
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