Toxicokinetics of monochloroacetic acid

a whole-body autoradiography study

Hari K. Bhat, Ahmed E. Ahmed, Ghulam Ansari

Research output: Contribution to journalArticle

18 Citations (Scopus)

Abstract

Monochloroacetic acid (MCA) is a toxic chemical used as a herbicide and in the synthesis of various organic compounds. MCA has also been shown to be present in chlorinated drinking waters. In order to understand the mechanism of MCA toxicity, we studied the tissue distribution of [1-14C]MCA in rats, by whole-body autoradiography technique. Male Sprague-Dawley rats were given a tracer dose of [1-14C]MCA [6.8 μg/100 g (40 μCi) body weight] by tail vein and euthanized at different time intervals (5 min, 1, 4, 12, 24 and 48 h). The animals were embedded in carboxymethyl cellulose and frozen immediately. Frozen animals were sectioned and processed using whole-body autoradiographic techniques. Analysis of developed sections showed that at 5 min, there was a rapid accumulation of 14C-activity in the kidney cortex and stomach walls. The radioactivity was rapidly removed from the circulation. There was high accumulation of 14C-activity in the myocardial tissues. The liver was also loaded with MCA and/or its metabolites. After 1 h following administration of [14C]MCA, radioactivity was extensively excreted into the small intestinal lumen. The accumulation of 14C-activity in the brain, thymus, salivary glands and tongue was prominent at 1 h. After 4 h the liver and other tissues started to eliminate most of the radioactivity. Contrary to other tissues, however, the central nervous system, thymus and pancreas started to accumulate the radioactivity at later time periods. These observations suggest the accumulation of MCA and/or its metabolites into hydrophilic tissues at earlier time periods and into lipophilic tissues at later times.

Original languageEnglish (US)
Pages (from-to)35-43
Number of pages9
JournalToxicology
Volume63
Issue number1
DOIs
StatePublished - 1990
Externally publishedYes

Fingerprint

Autoradiography
Radioactivity
Tissue
Thymus
Metabolites
Liver
Thymus Gland
Rats
Animals
Synthetic Chemistry Techniques
Kidney Cortex
Carboxymethylcellulose Sodium
chloroacetic acid
Toxicokinetics
Poisons
Neurology
Herbicides
Tissue Distribution
Salivary Glands
Organic compounds

Keywords

  • Distribution
  • Monochloroacetic acid
  • Whole-body Autoradiography

ASJC Scopus subject areas

  • Toxicology

Cite this

Toxicokinetics of monochloroacetic acid : a whole-body autoradiography study. / Bhat, Hari K.; Ahmed, Ahmed E.; Ansari, Ghulam.

In: Toxicology, Vol. 63, No. 1, 1990, p. 35-43.

Research output: Contribution to journalArticle

Bhat, Hari K. ; Ahmed, Ahmed E. ; Ansari, Ghulam. / Toxicokinetics of monochloroacetic acid : a whole-body autoradiography study. In: Toxicology. 1990 ; Vol. 63, No. 1. pp. 35-43.
@article{e3c1b89685c84a3e928a5f0cec50369f,
title = "Toxicokinetics of monochloroacetic acid: a whole-body autoradiography study",
abstract = "Monochloroacetic acid (MCA) is a toxic chemical used as a herbicide and in the synthesis of various organic compounds. MCA has also been shown to be present in chlorinated drinking waters. In order to understand the mechanism of MCA toxicity, we studied the tissue distribution of [1-14C]MCA in rats, by whole-body autoradiography technique. Male Sprague-Dawley rats were given a tracer dose of [1-14C]MCA [6.8 μg/100 g (40 μCi) body weight] by tail vein and euthanized at different time intervals (5 min, 1, 4, 12, 24 and 48 h). The animals were embedded in carboxymethyl cellulose and frozen immediately. Frozen animals were sectioned and processed using whole-body autoradiographic techniques. Analysis of developed sections showed that at 5 min, there was a rapid accumulation of 14C-activity in the kidney cortex and stomach walls. The radioactivity was rapidly removed from the circulation. There was high accumulation of 14C-activity in the myocardial tissues. The liver was also loaded with MCA and/or its metabolites. After 1 h following administration of [14C]MCA, radioactivity was extensively excreted into the small intestinal lumen. The accumulation of 14C-activity in the brain, thymus, salivary glands and tongue was prominent at 1 h. After 4 h the liver and other tissues started to eliminate most of the radioactivity. Contrary to other tissues, however, the central nervous system, thymus and pancreas started to accumulate the radioactivity at later time periods. These observations suggest the accumulation of MCA and/or its metabolites into hydrophilic tissues at earlier time periods and into lipophilic tissues at later times.",
keywords = "Distribution, Monochloroacetic acid, Whole-body Autoradiography",
author = "Bhat, {Hari K.} and Ahmed, {Ahmed E.} and Ghulam Ansari",
year = "1990",
doi = "10.1016/0300-483X(90)90066-P",
language = "English (US)",
volume = "63",
pages = "35--43",
journal = "Toxicology",
issn = "0300-483X",
publisher = "Elsevier Ireland Ltd",
number = "1",

}

TY - JOUR

T1 - Toxicokinetics of monochloroacetic acid

T2 - a whole-body autoradiography study

AU - Bhat, Hari K.

AU - Ahmed, Ahmed E.

AU - Ansari, Ghulam

PY - 1990

Y1 - 1990

N2 - Monochloroacetic acid (MCA) is a toxic chemical used as a herbicide and in the synthesis of various organic compounds. MCA has also been shown to be present in chlorinated drinking waters. In order to understand the mechanism of MCA toxicity, we studied the tissue distribution of [1-14C]MCA in rats, by whole-body autoradiography technique. Male Sprague-Dawley rats were given a tracer dose of [1-14C]MCA [6.8 μg/100 g (40 μCi) body weight] by tail vein and euthanized at different time intervals (5 min, 1, 4, 12, 24 and 48 h). The animals were embedded in carboxymethyl cellulose and frozen immediately. Frozen animals were sectioned and processed using whole-body autoradiographic techniques. Analysis of developed sections showed that at 5 min, there was a rapid accumulation of 14C-activity in the kidney cortex and stomach walls. The radioactivity was rapidly removed from the circulation. There was high accumulation of 14C-activity in the myocardial tissues. The liver was also loaded with MCA and/or its metabolites. After 1 h following administration of [14C]MCA, radioactivity was extensively excreted into the small intestinal lumen. The accumulation of 14C-activity in the brain, thymus, salivary glands and tongue was prominent at 1 h. After 4 h the liver and other tissues started to eliminate most of the radioactivity. Contrary to other tissues, however, the central nervous system, thymus and pancreas started to accumulate the radioactivity at later time periods. These observations suggest the accumulation of MCA and/or its metabolites into hydrophilic tissues at earlier time periods and into lipophilic tissues at later times.

AB - Monochloroacetic acid (MCA) is a toxic chemical used as a herbicide and in the synthesis of various organic compounds. MCA has also been shown to be present in chlorinated drinking waters. In order to understand the mechanism of MCA toxicity, we studied the tissue distribution of [1-14C]MCA in rats, by whole-body autoradiography technique. Male Sprague-Dawley rats were given a tracer dose of [1-14C]MCA [6.8 μg/100 g (40 μCi) body weight] by tail vein and euthanized at different time intervals (5 min, 1, 4, 12, 24 and 48 h). The animals were embedded in carboxymethyl cellulose and frozen immediately. Frozen animals were sectioned and processed using whole-body autoradiographic techniques. Analysis of developed sections showed that at 5 min, there was a rapid accumulation of 14C-activity in the kidney cortex and stomach walls. The radioactivity was rapidly removed from the circulation. There was high accumulation of 14C-activity in the myocardial tissues. The liver was also loaded with MCA and/or its metabolites. After 1 h following administration of [14C]MCA, radioactivity was extensively excreted into the small intestinal lumen. The accumulation of 14C-activity in the brain, thymus, salivary glands and tongue was prominent at 1 h. After 4 h the liver and other tissues started to eliminate most of the radioactivity. Contrary to other tissues, however, the central nervous system, thymus and pancreas started to accumulate the radioactivity at later time periods. These observations suggest the accumulation of MCA and/or its metabolites into hydrophilic tissues at earlier time periods and into lipophilic tissues at later times.

KW - Distribution

KW - Monochloroacetic acid

KW - Whole-body Autoradiography

UR - http://www.scopus.com/inward/record.url?scp=0025357939&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=0025357939&partnerID=8YFLogxK

U2 - 10.1016/0300-483X(90)90066-P

DO - 10.1016/0300-483X(90)90066-P

M3 - Article

VL - 63

SP - 35

EP - 43

JO - Toxicology

JF - Toxicology

SN - 0300-483X

IS - 1

ER -