Toxoplasma gondii: Protective immunity against toxoplasmosis with recombinant actin depolymerizing factor protein in BALB/c mice

Xiangsheng Huang, Jianhua Li, Guocai Zhang, Pengtao Gong, Ju Yang, Xichen Zhang

Research output: Contribution to journalArticle

19 Scopus citations

Abstract

Toxoplasmosis is one of the most world-wide spread zoonosis representing a very serious clinical and veterinary problem. There is still need for vaccines for toxoplasmosis. In the present study, we evaluated the protective efficacy of a recombinant actin depolymerizing factor (ADF) subunit vaccine against Toxoplasma gondii infection in BALB/c mice. The recombinant T. gondii ADF protein (rADF) was expressed in Escherichia coli and used as antigens for BALB/c mice immunization. The results indicated that specific antibody and the increased percentage of CD4 + T lymphocyte were found in vaccinated BALB/c mice with rADF, when compared with adjuvant or PBS groups. After challenged with T. gondii (RH strain) tachyzoites, the survival time of the mice in rADF group was longer than those in the control group. The numbers of brain cysts of the mice in rADF group reduced significantly when compared with those in control groups (P<0.05), and the rate of reduction could reach to around 30%. These results suggest that rADF can generate protective immunity against T. gondii infection in BALB/c mice.

Original languageEnglish (US)
Pages (from-to)218-222
Number of pages5
JournalExperimental Parasitology
Volume130
Issue number3
DOIs
StatePublished - Mar 1 2012

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Keywords

  • Actin depolymerizing factor
  • BALB/c mice
  • Recombinant protein
  • Toxoplasma gondii

ASJC Scopus subject areas

  • Parasitology
  • Immunology
  • Infectious Diseases

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