Tracheal venous blood and lymph collection

A model to study airway injury in sheep

R. E. Barrow, S. E. Morris, H. A. Linares, David Herndon

Research output: Contribution to journalArticle

7 Citations (Scopus)

Abstract

Airway injury is a frequent result of the inhalation or aspiration of toxic material. Although upper airway damage can be identified endoscopically, pathophysiological changes are difficult to evaluate. This paper describes an animal model in which changes in tracheal blood and lymph flow rates, wet-to-dry weight ratios, and lymph-to-plasma protein ratios can be evaluated after injury. In this model, 12 cm of the cervical trachea were isolated using a double-cuffed endotracheal tube and injured with cotton smoke at near room temperature. Injury to the trachea was evaluated in twenty-five anesthetized sheep 4 (n = 3), 8 (n = 3), 24 (n = 3), 48 (n = 3), 96 (n = 3), and 192 (n = 2) h after smoke exposure and compared with sham control animals (n = 8). A significant increase in tracheal venous blood flow from 1.3 ± 0.4 (SD) ml · min-1 · cm-1 for the noninjured trachea to 2.8 ± 1.2 was noted 24 h after injury (P < 0.01). Lymph flow significantly increased from 1.3 ± 0.4 μl · min-1 · cm-1 for the noninjured trachea to 9.8 ± 3.3 24 h after injury while wet-to-dry weight ratios were elevated from 3.0 ± 0.2 for noninjured trachea to 4.6 ± 0.9 from 4 to 24 h after injury (P < 0.01) and decreased to 3.7 ± 0.5 by 96 h. Cast material consisting of airway exudate, cellular debris, and intact ciliated epithelial cells was both expectorated and found in the trachea when the animals were killed. Lymph-to-plasma total protein and albumin ratios did not decrease with a sevenfold increase in tracheal lymph flow. This indicates that, in addition to a severe injury to the epithelium, there was a change in the permeability of the trachea microvascular system that was most evident 4-24 h after injury.

Original languageEnglish (US)
Pages (from-to)1645-1649
Number of pages5
JournalJournal of Applied Physiology
Volume70
Issue number4
StatePublished - 1991

Fingerprint

Lymph
Trachea
Sheep
Wounds and Injuries
Smoke
Blood Proteins
Weights and Measures
Poisons
Capillary Permeability
Exudates and Transudates
Serum Albumin
Inhalation
Epithelium
Animal Models
Epithelial Cells
Temperature

Keywords

  • isolated tracheal injury in sheep
  • toxic smoke inhalation
  • tracheal blood flow
  • tracheal lymph flow

ASJC Scopus subject areas

  • Physiology
  • Endocrinology
  • Orthopedics and Sports Medicine
  • Physical Therapy, Sports Therapy and Rehabilitation

Cite this

Barrow, R. E., Morris, S. E., Linares, H. A., & Herndon, D. (1991). Tracheal venous blood and lymph collection: A model to study airway injury in sheep. Journal of Applied Physiology, 70(4), 1645-1649.

Tracheal venous blood and lymph collection : A model to study airway injury in sheep. / Barrow, R. E.; Morris, S. E.; Linares, H. A.; Herndon, David.

In: Journal of Applied Physiology, Vol. 70, No. 4, 1991, p. 1645-1649.

Research output: Contribution to journalArticle

Barrow, RE, Morris, SE, Linares, HA & Herndon, D 1991, 'Tracheal venous blood and lymph collection: A model to study airway injury in sheep', Journal of Applied Physiology, vol. 70, no. 4, pp. 1645-1649.
Barrow, R. E. ; Morris, S. E. ; Linares, H. A. ; Herndon, David. / Tracheal venous blood and lymph collection : A model to study airway injury in sheep. In: Journal of Applied Physiology. 1991 ; Vol. 70, No. 4. pp. 1645-1649.
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