Traditional and novel approaches to flavivirus vaccines

Konstantin V. Pugachev, Farshad Guirakhoo, Dennis W. Trent, Thomas P. Monath

Research output: Contribution to journalArticle

70 Citations (Scopus)

Abstract

Yellow fever, dengue, Japanese encephalitis and tick-borne encephalitis viruses are the medically most important members of the Flavivirus genus composed primarily of arboviruses. In this paper, we review the commercially available traditional flavivirus vaccines against yellow fever, Japanese encephalitis, and tick-borne encephalitis, as well as modern approaches to flavivirus vaccines. Formalin inactivation technology has been employed to produce killed vaccines. Flaviviruses have been attenuated by multiple passages in animal tissues and cell cultures to produce empirical live attenuated vaccines. The use of traditional methods is being pursued to develop vaccines against other flavivirus diseases, such as dengue, and to improve existing vaccines, such as for Japanese encephalitis. With the recent development of infectious clones, rational approaches to attenuated flavivirus vaccines have employed the introduction of specific mutations into wild type viruses and chimerisation between different viruses. Novel methods for delivery of live vaccines, such as inoculation of infectious DNA or RNA, have been described. Other approaches, such as the construction of protein subunit, expression vector-based and naked DNA vaccines, have been proposed to create alternate vaccine candidates.

Original languageEnglish (US)
Pages (from-to)567-582
Number of pages16
JournalInternational Journal for Parasitology
Volume33
Issue number5-6
DOIs
StatePublished - May 2003

Fingerprint

Flavivirus
Vaccines
Japanese Encephalitis
Attenuated Vaccines
Dengue
Yellow Fever Vaccine
Tick-Borne Encephalitis Viruses
Tick-Borne Encephalitis
Viruses
Yellow Fever
Arboviruses
Inactivated Vaccines
DNA Vaccines
Protein Subunits
Formaldehyde
Clone Cells
Cell Culture Techniques
RNA
Technology
Mutation

Keywords

  • Chimeric
  • DNA vaccine
  • Flavivirus
  • Live attenuated
  • Technology
  • Vaccine

ASJC Scopus subject areas

  • Parasitology
  • Infectious Diseases

Cite this

Pugachev, K. V., Guirakhoo, F., Trent, D. W., & Monath, T. P. (2003). Traditional and novel approaches to flavivirus vaccines. International Journal for Parasitology, 33(5-6), 567-582. https://doi.org/10.1016/S0020-7519(03)00063-8

Traditional and novel approaches to flavivirus vaccines. / Pugachev, Konstantin V.; Guirakhoo, Farshad; Trent, Dennis W.; Monath, Thomas P.

In: International Journal for Parasitology, Vol. 33, No. 5-6, 05.2003, p. 567-582.

Research output: Contribution to journalArticle

Pugachev, KV, Guirakhoo, F, Trent, DW & Monath, TP 2003, 'Traditional and novel approaches to flavivirus vaccines', International Journal for Parasitology, vol. 33, no. 5-6, pp. 567-582. https://doi.org/10.1016/S0020-7519(03)00063-8
Pugachev, Konstantin V. ; Guirakhoo, Farshad ; Trent, Dennis W. ; Monath, Thomas P. / Traditional and novel approaches to flavivirus vaccines. In: International Journal for Parasitology. 2003 ; Vol. 33, No. 5-6. pp. 567-582.
@article{8ebdb23bf8324079813bbd787f61bce7,
title = "Traditional and novel approaches to flavivirus vaccines",
abstract = "Yellow fever, dengue, Japanese encephalitis and tick-borne encephalitis viruses are the medically most important members of the Flavivirus genus composed primarily of arboviruses. In this paper, we review the commercially available traditional flavivirus vaccines against yellow fever, Japanese encephalitis, and tick-borne encephalitis, as well as modern approaches to flavivirus vaccines. Formalin inactivation technology has been employed to produce killed vaccines. Flaviviruses have been attenuated by multiple passages in animal tissues and cell cultures to produce empirical live attenuated vaccines. The use of traditional methods is being pursued to develop vaccines against other flavivirus diseases, such as dengue, and to improve existing vaccines, such as for Japanese encephalitis. With the recent development of infectious clones, rational approaches to attenuated flavivirus vaccines have employed the introduction of specific mutations into wild type viruses and chimerisation between different viruses. Novel methods for delivery of live vaccines, such as inoculation of infectious DNA or RNA, have been described. Other approaches, such as the construction of protein subunit, expression vector-based and naked DNA vaccines, have been proposed to create alternate vaccine candidates.",
keywords = "Chimeric, DNA vaccine, Flavivirus, Live attenuated, Technology, Vaccine",
author = "Pugachev, {Konstantin V.} and Farshad Guirakhoo and Trent, {Dennis W.} and Monath, {Thomas P.}",
year = "2003",
month = "5",
doi = "10.1016/S0020-7519(03)00063-8",
language = "English (US)",
volume = "33",
pages = "567--582",
journal = "International Journal for Parasitology",
issn = "0020-7519",
publisher = "Elsevier Limited",
number = "5-6",

}

TY - JOUR

T1 - Traditional and novel approaches to flavivirus vaccines

AU - Pugachev, Konstantin V.

AU - Guirakhoo, Farshad

AU - Trent, Dennis W.

AU - Monath, Thomas P.

PY - 2003/5

Y1 - 2003/5

N2 - Yellow fever, dengue, Japanese encephalitis and tick-borne encephalitis viruses are the medically most important members of the Flavivirus genus composed primarily of arboviruses. In this paper, we review the commercially available traditional flavivirus vaccines against yellow fever, Japanese encephalitis, and tick-borne encephalitis, as well as modern approaches to flavivirus vaccines. Formalin inactivation technology has been employed to produce killed vaccines. Flaviviruses have been attenuated by multiple passages in animal tissues and cell cultures to produce empirical live attenuated vaccines. The use of traditional methods is being pursued to develop vaccines against other flavivirus diseases, such as dengue, and to improve existing vaccines, such as for Japanese encephalitis. With the recent development of infectious clones, rational approaches to attenuated flavivirus vaccines have employed the introduction of specific mutations into wild type viruses and chimerisation between different viruses. Novel methods for delivery of live vaccines, such as inoculation of infectious DNA or RNA, have been described. Other approaches, such as the construction of protein subunit, expression vector-based and naked DNA vaccines, have been proposed to create alternate vaccine candidates.

AB - Yellow fever, dengue, Japanese encephalitis and tick-borne encephalitis viruses are the medically most important members of the Flavivirus genus composed primarily of arboviruses. In this paper, we review the commercially available traditional flavivirus vaccines against yellow fever, Japanese encephalitis, and tick-borne encephalitis, as well as modern approaches to flavivirus vaccines. Formalin inactivation technology has been employed to produce killed vaccines. Flaviviruses have been attenuated by multiple passages in animal tissues and cell cultures to produce empirical live attenuated vaccines. The use of traditional methods is being pursued to develop vaccines against other flavivirus diseases, such as dengue, and to improve existing vaccines, such as for Japanese encephalitis. With the recent development of infectious clones, rational approaches to attenuated flavivirus vaccines have employed the introduction of specific mutations into wild type viruses and chimerisation between different viruses. Novel methods for delivery of live vaccines, such as inoculation of infectious DNA or RNA, have been described. Other approaches, such as the construction of protein subunit, expression vector-based and naked DNA vaccines, have been proposed to create alternate vaccine candidates.

KW - Chimeric

KW - DNA vaccine

KW - Flavivirus

KW - Live attenuated

KW - Technology

KW - Vaccine

UR - http://www.scopus.com/inward/record.url?scp=0038024238&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=0038024238&partnerID=8YFLogxK

U2 - 10.1016/S0020-7519(03)00063-8

DO - 10.1016/S0020-7519(03)00063-8

M3 - Article

VL - 33

SP - 567

EP - 582

JO - International Journal for Parasitology

JF - International Journal for Parasitology

SN - 0020-7519

IS - 5-6

ER -