Trafficking of ODV-E66 is mediated via a sorting motif and other viral proteins

Facilitated trafficking to the inner nuclear membrane

Sharon C. Braunagel, Shawn T. Williamson, Suraj Saksena, Zhenping Zhong, William Russell, David H. Russell, Max D. Summers

Research output: Contribution to journalArticle

75 Citations (Scopus)

Abstract

The N-terminal 33 aa of the envelope protein ODV-E66 are sufficient to traffic fusion proteins to intranuclear membranes and the ODV envelope during infection with Autographa californica nucleopolyhedrovirus. This sequence has two distinct features: (i) an extremely hydrophobic sequence of 18 aa and (ii) positively charged amino acids close to the C-terminal end of the hydrophobic sequence. In the absence of infection, this sequence is sufficient to promote protein accumulation at the inner nuclear membrane. Covalent cross-linking results show that the lysines of the motif are proximal to FP25K and/or BV/ODV-E26 during transit from the endoplasmic reticulum to the nuclear envelope. We propose that the 33 aa comprise a signature for sorting proteins to the inner nuclear membrane (sorting motif) and that, unlike other resident proteins of the inner nuclear membrane, ODV-E66 and sorting-motif fusions do not randomly diffuse from their site of insertion at the endoplasmic reticulum to the nuclear envelope and viral-induced intranuclear membranes. Rather, during infection, trafficking is mediated by protein-protein interactions.

Original languageEnglish (US)
Pages (from-to)8372-8377
Number of pages6
JournalProceedings of the National Academy of Sciences of the United States of America
Volume101
Issue number22
DOIs
StatePublished - Jun 1 2004
Externally publishedYes

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Nuclear Envelope
Viral Proteins
Protein Transport
Proteins
Endoplasmic Reticulum
Infection
Nucleopolyhedrovirus
Membranes
Lysine
Amino Acids

ASJC Scopus subject areas

  • Genetics
  • General

Cite this

Trafficking of ODV-E66 is mediated via a sorting motif and other viral proteins : Facilitated trafficking to the inner nuclear membrane. / Braunagel, Sharon C.; Williamson, Shawn T.; Saksena, Suraj; Zhong, Zhenping; Russell, William; Russell, David H.; Summers, Max D.

In: Proceedings of the National Academy of Sciences of the United States of America, Vol. 101, No. 22, 01.06.2004, p. 8372-8377.

Research output: Contribution to journalArticle

Braunagel, Sharon C. ; Williamson, Shawn T. ; Saksena, Suraj ; Zhong, Zhenping ; Russell, William ; Russell, David H. ; Summers, Max D. / Trafficking of ODV-E66 is mediated via a sorting motif and other viral proteins : Facilitated trafficking to the inner nuclear membrane. In: Proceedings of the National Academy of Sciences of the United States of America. 2004 ; Vol. 101, No. 22. pp. 8372-8377.
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