Transcript levels of the Saccharomyes cerevisiae DNA repair gene RAD23 increase in response to UV light andin meiosis but remain constant in the mitotic cell cycle

Kiran Madura, Satya Prakash

Research output: Contribution to journalArticle

53 Scopus citations

Abstract

The RAD23 gene of Saccharomyces cerevisiae is required for excision-repair of UV damaged DNA. In this paper, we determine the location of the RAD23 genein a cloned DNA fragment, identify the 1.6 kb RAD23 transcript, and examine RAD23 transcript levels in UV damaged cells, during the mitotic cell cycle, and in meiosis. The RAD23 mRNA levels are elevated 5-fold between 30 to 60 min after 37 J/m2 of UV light. RAD23 mRNA levels rise over 6-fold during meiosis at a stagecoincident with high levels of genetic recombination. This response is specific to sporulation competent MATa/MATα diploid cells, and is not observed in asporogenous MATa/MATα diploids. RAD23 mRNA levels, however, remain constant during the mitotic cell cycle.

Original languageEnglish (US)
Pages (from-to)4737-4742
Number of pages6
JournalNucleic acids research
Volume18
Issue number16
DOIs
StatePublished - Aug 25 1990
Externally publishedYes

ASJC Scopus subject areas

  • Genetics

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