Transcript levels of the Saccharomyes cerevisiae DNA repair gene RAD23 increase in response to UV light andin meiosis but remain constant in the mitotic cell cycle

Kiran Madura; Satya Prakash

doi:10.1093/nar/18.16.4737

Transcript levels of the Saccharomyes cerevisiae DNA repair gene RAD23 increase in response to UV light andin meiosis but remain constant in the mitotic cell cycle

Kiran Madura, Satya Prakash

Research output: Contribution to journal › Article › peer-review

58 Scopus citations

Abstract

The RAD23 gene of Saccharomyces cerevisiae is required for excision-repair of UV damaged DNA. In this paper, we determine the location of the RAD23 genein a cloned DNA fragment, identify the 1.6 kb RAD23 transcript, and examine RAD23 transcript levels in UV damaged cells, during the mitotic cell cycle, and in meiosis. The RAD23 mRNA levels are elevated 5-fold between 30 to 60 min after 37 J/m² of UV light. RAD23 mRNA levels rise over 6-fold during meiosis at a stagecoincident with high levels of genetic recombination. This response is specific to sporulation competent MATa/MATα diploid cells, and is not observed in asporogenous MATa/MATα diploids. RAD23 mRNA levels, however, remain constant during the mitotic cell cycle.

Original language	English (US)
Pages (from-to)	4737-4742
Number of pages	6
Journal	Nucleic acids research
Volume	18
Issue number	16
DOIs	https://doi.org/10.1093/nar/18.16.4737
State	Published - Aug 25 1990
Externally published	Yes

ASJC Scopus subject areas

Genetics

Access to Document

10.1093/nar/18.16.4737

Cite this

@article{560420fe0112430a80a8c03e205df288,

title = "Transcript levels of the Saccharomyes cerevisiae DNA repair gene RAD23 increase in response to UV light andin meiosis but remain constant in the mitotic cell cycle",

abstract = "The RAD23 gene of Saccharomyces cerevisiae is required for excision-repair of UV damaged DNA. In this paper, we determine the location of the RAD23 genein a cloned DNA fragment, identify the 1.6 kb RAD23 transcript, and examine RAD23 transcript levels in UV damaged cells, during the mitotic cell cycle, and in meiosis. The RAD23 mRNA levels are elevated 5-fold between 30 to 60 min after 37 J/m2 of UV light. RAD23 mRNA levels rise over 6-fold during meiosis at a stagecoincident with high levels of genetic recombination. This response is specific to sporulation competent MATa/MATα diploid cells, and is not observed in asporogenous MATa/MATα diploids. RAD23 mRNA levels, however, remain constant during the mitotic cell cycle.",

author = "Kiran Madura and Satya Prakash",

note = "Funding Information: We are grateful to F. Sherman and L. Melnick for providing plasmids and the RAD23 nucleotide sequence information prior to publication, Janet Ives for expert technical assistance, and P. Sadhale for the initial characterization of RAD23. We thank J. Game, I. Herskowitz, and J. Huberman for providing strains. This work was supported by Public Health Service grant CA 41261 from the National Cancer Institute.",

year = "1990",

month = aug,

day = "25",

doi = "10.1093/nar/18.16.4737",

language = "English (US)",

volume = "18",

pages = "4737--4742",

journal = "Nucleic acids research",

issn = "0305-1048",

publisher = "Oxford University Press",

number = "16",

}

TY - JOUR

T1 - Transcript levels of the Saccharomyes cerevisiae DNA repair gene RAD23 increase in response to UV light andin meiosis but remain constant in the mitotic cell cycle

AU - Madura, Kiran

AU - Prakash, Satya

N1 - Funding Information: We are grateful to F. Sherman and L. Melnick for providing plasmids and the RAD23 nucleotide sequence information prior to publication, Janet Ives for expert technical assistance, and P. Sadhale for the initial characterization of RAD23. We thank J. Game, I. Herskowitz, and J. Huberman for providing strains. This work was supported by Public Health Service grant CA 41261 from the National Cancer Institute.

PY - 1990/8/25

Y1 - 1990/8/25

N2 - The RAD23 gene of Saccharomyces cerevisiae is required for excision-repair of UV damaged DNA. In this paper, we determine the location of the RAD23 genein a cloned DNA fragment, identify the 1.6 kb RAD23 transcript, and examine RAD23 transcript levels in UV damaged cells, during the mitotic cell cycle, and in meiosis. The RAD23 mRNA levels are elevated 5-fold between 30 to 60 min after 37 J/m2 of UV light. RAD23 mRNA levels rise over 6-fold during meiosis at a stagecoincident with high levels of genetic recombination. This response is specific to sporulation competent MATa/MATα diploid cells, and is not observed in asporogenous MATa/MATα diploids. RAD23 mRNA levels, however, remain constant during the mitotic cell cycle.

AB - The RAD23 gene of Saccharomyces cerevisiae is required for excision-repair of UV damaged DNA. In this paper, we determine the location of the RAD23 genein a cloned DNA fragment, identify the 1.6 kb RAD23 transcript, and examine RAD23 transcript levels in UV damaged cells, during the mitotic cell cycle, and in meiosis. The RAD23 mRNA levels are elevated 5-fold between 30 to 60 min after 37 J/m2 of UV light. RAD23 mRNA levels rise over 6-fold during meiosis at a stagecoincident with high levels of genetic recombination. This response is specific to sporulation competent MATa/MATα diploid cells, and is not observed in asporogenous MATa/MATα diploids. RAD23 mRNA levels, however, remain constant during the mitotic cell cycle.

UR - http://www.scopus.com/inward/record.url?scp=0025033463&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=0025033463&partnerID=8YFLogxK

U2 - 10.1093/nar/18.16.4737

DO - 10.1093/nar/18.16.4737

M3 - Article

C2 - 2204027

AN - SCOPUS:0025033463

SN - 0305-1048

VL - 18

SP - 4737

EP - 4742

JO - Nucleic acids research

JF - Nucleic acids research

IS - 16

ER -

Transcript levels of the Saccharomyes cerevisiae DNA repair gene RAD23 increase in response to UV light andin meiosis but remain constant in the mitotic cell cycle

Abstract

ASJC Scopus subject areas

Access to Document

Other files and links

Fingerprint

Cite this