Abstract
Nitric Oxide (NO) and its associated reactive nitrogen species (RNS) produce nitrosative stress under various pathophysiological conditions in eukaryotes. The fission yeast Schizosaccharomyces pombe regulates stress response mainly through the Sty1-Atf1 MAP Kinase pathway. The present study deals with the role of transcription factor Atf1 and Sty1 in S. pombe under nitrosative stress. In this study, exposure to an NO donor resulted in S-phase slowdown with associated mitotic block in S. pombe. Deletion of sty1 and atf1 in S. pombe had differential growth sensitivity towards NO donor. Both Sty1 and Atf1 were involved in regulating mitotic slowdown in S. pombe under nitrosative stress. Experimental data obtained in this study reveals a novel role of Atf1 in initiating the replication slowdown in S. pombe under nitrosative stress. Both Sty1 and Atf1 were accumulated in the nucleus in S. pombe under nitrosative stress in a concentration and time dependent manner. Atf1 is also found to be nuclear delocalized under longer nitrosative stress.
| Original language | English (US) |
|---|---|
| Pages (from-to) | 82-90 |
| Number of pages | 9 |
| Journal | Microbiological Research |
| Volume | 206 |
| DOIs | |
| State | Published - Jan 1 2018 |
| Externally published | Yes |
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Keywords
- Atf1
- Nitric oxide
- Nitrosative stress
- Nuclear localization
- Schizosaccharomyces pombe
- Sty1
ASJC Scopus subject areas
- Microbiology
Cite this
Transcription factors Atf1 and Sty1 promote stress tolerance under nitrosative stress in Schizosaccharomyces pombe. / Kar, Puranjoy; Biswas, Pranjal; Patra, Sourav Kumar; Ghosh, Sanjay.
In: Microbiological Research, Vol. 206, 01.01.2018, p. 82-90.Research output: Contribution to journal › Article
}
TY - JOUR
T1 - Transcription factors Atf1 and Sty1 promote stress tolerance under nitrosative stress in Schizosaccharomyces pombe
AU - Kar, Puranjoy
AU - Biswas, Pranjal
AU - Patra, Sourav Kumar
AU - Ghosh, Sanjay
PY - 2018/1/1
Y1 - 2018/1/1
N2 - Nitric Oxide (NO) and its associated reactive nitrogen species (RNS) produce nitrosative stress under various pathophysiological conditions in eukaryotes. The fission yeast Schizosaccharomyces pombe regulates stress response mainly through the Sty1-Atf1 MAP Kinase pathway. The present study deals with the role of transcription factor Atf1 and Sty1 in S. pombe under nitrosative stress. In this study, exposure to an NO donor resulted in S-phase slowdown with associated mitotic block in S. pombe. Deletion of sty1 and atf1 in S. pombe had differential growth sensitivity towards NO donor. Both Sty1 and Atf1 were involved in regulating mitotic slowdown in S. pombe under nitrosative stress. Experimental data obtained in this study reveals a novel role of Atf1 in initiating the replication slowdown in S. pombe under nitrosative stress. Both Sty1 and Atf1 were accumulated in the nucleus in S. pombe under nitrosative stress in a concentration and time dependent manner. Atf1 is also found to be nuclear delocalized under longer nitrosative stress.
AB - Nitric Oxide (NO) and its associated reactive nitrogen species (RNS) produce nitrosative stress under various pathophysiological conditions in eukaryotes. The fission yeast Schizosaccharomyces pombe regulates stress response mainly through the Sty1-Atf1 MAP Kinase pathway. The present study deals with the role of transcription factor Atf1 and Sty1 in S. pombe under nitrosative stress. In this study, exposure to an NO donor resulted in S-phase slowdown with associated mitotic block in S. pombe. Deletion of sty1 and atf1 in S. pombe had differential growth sensitivity towards NO donor. Both Sty1 and Atf1 were involved in regulating mitotic slowdown in S. pombe under nitrosative stress. Experimental data obtained in this study reveals a novel role of Atf1 in initiating the replication slowdown in S. pombe under nitrosative stress. Both Sty1 and Atf1 were accumulated in the nucleus in S. pombe under nitrosative stress in a concentration and time dependent manner. Atf1 is also found to be nuclear delocalized under longer nitrosative stress.
KW - Atf1
KW - Nitric oxide
KW - Nitrosative stress
KW - Nuclear localization
KW - Schizosaccharomyces pombe
KW - Sty1
UR - http://www.scopus.com/inward/record.url?scp=85031758992&partnerID=8YFLogxK
UR - http://www.scopus.com/inward/citedby.url?scp=85031758992&partnerID=8YFLogxK
U2 - 10.1016/j.micres.2017.10.002
DO - 10.1016/j.micres.2017.10.002
M3 - Article
C2 - 29146263
AN - SCOPUS:85031758992
VL - 206
SP - 82
EP - 90
JO - Microbiological Research
JF - Microbiological Research
SN - 0944-5013
ER -