Transcription factors Atf1 and Sty1 promote stress tolerance under nitrosative stress in Schizosaccharomyces pombe

Puranjoy Kar, Pranjal Biswas, Sourav Kumar Patra, Sanjay Ghosh

Research output: Contribution to journalArticle

1 Citation (Scopus)

Abstract

Nitric Oxide (NO) and its associated reactive nitrogen species (RNS) produce nitrosative stress under various pathophysiological conditions in eukaryotes. The fission yeast Schizosaccharomyces pombe regulates stress response mainly through the Sty1-Atf1 MAP Kinase pathway. The present study deals with the role of transcription factor Atf1 and Sty1 in S. pombe under nitrosative stress. In this study, exposure to an NO donor resulted in S-phase slowdown with associated mitotic block in S. pombe. Deletion of sty1 and atf1 in S. pombe had differential growth sensitivity towards NO donor. Both Sty1 and Atf1 were involved in regulating mitotic slowdown in S. pombe under nitrosative stress. Experimental data obtained in this study reveals a novel role of Atf1 in initiating the replication slowdown in S. pombe under nitrosative stress. Both Sty1 and Atf1 were accumulated in the nucleus in S. pombe under nitrosative stress in a concentration and time dependent manner. Atf1 is also found to be nuclear delocalized under longer nitrosative stress.

Original languageEnglish (US)
Pages (from-to)82-90
Number of pages9
JournalMicrobiological Research
Volume206
DOIs
StatePublished - Jan 1 2018
Externally publishedYes

Fingerprint

Schizosaccharomyces
Transcription Factors
Nitric Oxide Donors
Reactive Nitrogen Species
Eukaryota
S Phase
Nitric Oxide
Phosphotransferases
Growth

Keywords

  • Atf1
  • Nitric oxide
  • Nitrosative stress
  • Nuclear localization
  • Schizosaccharomyces pombe
  • Sty1

ASJC Scopus subject areas

  • Microbiology

Cite this

Transcription factors Atf1 and Sty1 promote stress tolerance under nitrosative stress in Schizosaccharomyces pombe. / Kar, Puranjoy; Biswas, Pranjal; Patra, Sourav Kumar; Ghosh, Sanjay.

In: Microbiological Research, Vol. 206, 01.01.2018, p. 82-90.

Research output: Contribution to journalArticle

Kar, Puranjoy ; Biswas, Pranjal ; Patra, Sourav Kumar ; Ghosh, Sanjay. / Transcription factors Atf1 and Sty1 promote stress tolerance under nitrosative stress in Schizosaccharomyces pombe. In: Microbiological Research. 2018 ; Vol. 206. pp. 82-90.
@article{b9caa611ece345e7987d64466e9a2384,
title = "Transcription factors Atf1 and Sty1 promote stress tolerance under nitrosative stress in Schizosaccharomyces pombe",
abstract = "Nitric Oxide (NO) and its associated reactive nitrogen species (RNS) produce nitrosative stress under various pathophysiological conditions in eukaryotes. The fission yeast Schizosaccharomyces pombe regulates stress response mainly through the Sty1-Atf1 MAP Kinase pathway. The present study deals with the role of transcription factor Atf1 and Sty1 in S. pombe under nitrosative stress. In this study, exposure to an NO donor resulted in S-phase slowdown with associated mitotic block in S. pombe. Deletion of sty1 and atf1 in S. pombe had differential growth sensitivity towards NO donor. Both Sty1 and Atf1 were involved in regulating mitotic slowdown in S. pombe under nitrosative stress. Experimental data obtained in this study reveals a novel role of Atf1 in initiating the replication slowdown in S. pombe under nitrosative stress. Both Sty1 and Atf1 were accumulated in the nucleus in S. pombe under nitrosative stress in a concentration and time dependent manner. Atf1 is also found to be nuclear delocalized under longer nitrosative stress.",
keywords = "Atf1, Nitric oxide, Nitrosative stress, Nuclear localization, Schizosaccharomyces pombe, Sty1",
author = "Puranjoy Kar and Pranjal Biswas and Patra, {Sourav Kumar} and Sanjay Ghosh",
year = "2018",
month = "1",
day = "1",
doi = "10.1016/j.micres.2017.10.002",
language = "English (US)",
volume = "206",
pages = "82--90",
journal = "Microbiological Research",
issn = "0944-5013",
publisher = "Urban und Fischer Verlag Jena",

}

TY - JOUR

T1 - Transcription factors Atf1 and Sty1 promote stress tolerance under nitrosative stress in Schizosaccharomyces pombe

AU - Kar, Puranjoy

AU - Biswas, Pranjal

AU - Patra, Sourav Kumar

AU - Ghosh, Sanjay

PY - 2018/1/1

Y1 - 2018/1/1

N2 - Nitric Oxide (NO) and its associated reactive nitrogen species (RNS) produce nitrosative stress under various pathophysiological conditions in eukaryotes. The fission yeast Schizosaccharomyces pombe regulates stress response mainly through the Sty1-Atf1 MAP Kinase pathway. The present study deals with the role of transcription factor Atf1 and Sty1 in S. pombe under nitrosative stress. In this study, exposure to an NO donor resulted in S-phase slowdown with associated mitotic block in S. pombe. Deletion of sty1 and atf1 in S. pombe had differential growth sensitivity towards NO donor. Both Sty1 and Atf1 were involved in regulating mitotic slowdown in S. pombe under nitrosative stress. Experimental data obtained in this study reveals a novel role of Atf1 in initiating the replication slowdown in S. pombe under nitrosative stress. Both Sty1 and Atf1 were accumulated in the nucleus in S. pombe under nitrosative stress in a concentration and time dependent manner. Atf1 is also found to be nuclear delocalized under longer nitrosative stress.

AB - Nitric Oxide (NO) and its associated reactive nitrogen species (RNS) produce nitrosative stress under various pathophysiological conditions in eukaryotes. The fission yeast Schizosaccharomyces pombe regulates stress response mainly through the Sty1-Atf1 MAP Kinase pathway. The present study deals with the role of transcription factor Atf1 and Sty1 in S. pombe under nitrosative stress. In this study, exposure to an NO donor resulted in S-phase slowdown with associated mitotic block in S. pombe. Deletion of sty1 and atf1 in S. pombe had differential growth sensitivity towards NO donor. Both Sty1 and Atf1 were involved in regulating mitotic slowdown in S. pombe under nitrosative stress. Experimental data obtained in this study reveals a novel role of Atf1 in initiating the replication slowdown in S. pombe under nitrosative stress. Both Sty1 and Atf1 were accumulated in the nucleus in S. pombe under nitrosative stress in a concentration and time dependent manner. Atf1 is also found to be nuclear delocalized under longer nitrosative stress.

KW - Atf1

KW - Nitric oxide

KW - Nitrosative stress

KW - Nuclear localization

KW - Schizosaccharomyces pombe

KW - Sty1

UR - http://www.scopus.com/inward/record.url?scp=85031758992&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=85031758992&partnerID=8YFLogxK

U2 - 10.1016/j.micres.2017.10.002

DO - 10.1016/j.micres.2017.10.002

M3 - Article

VL - 206

SP - 82

EP - 90

JO - Microbiological Research

JF - Microbiological Research

SN - 0944-5013

ER -