Transcription factors Atf1 and Sty1 promote stress tolerance under nitrosative stress in Schizosaccharomyces pombe

Puranjoy Kar; Pranjal Biswas; Sourav Kumar Patra; Sanjay Ghosh

doi:10.1016/j.micres.2017.10.002

Transcription factors Atf1 and Sty1 promote stress tolerance under nitrosative stress in Schizosaccharomyces pombe

Puranjoy Kar, Pranjal Biswas, Sourav Kumar Patra, Sanjay Ghosh

Research output: Contribution to journal › Article › peer-review

8 Scopus citations

Abstract

Nitric Oxide (NO) and its associated reactive nitrogen species (RNS) produce nitrosative stress under various pathophysiological conditions in eukaryotes. The fission yeast Schizosaccharomyces pombe regulates stress response mainly through the Sty1-Atf1 MAP Kinase pathway. The present study deals with the role of transcription factor Atf1 and Sty1 in S. pombe under nitrosative stress. In this study, exposure to an NO donor resulted in S-phase slowdown with associated mitotic block in S. pombe. Deletion of sty1 and atf1 in S. pombe had differential growth sensitivity towards NO donor. Both Sty1 and Atf1 were involved in regulating mitotic slowdown in S. pombe under nitrosative stress. Experimental data obtained in this study reveals a novel role of Atf1 in initiating the replication slowdown in S. pombe under nitrosative stress. Both Sty1 and Atf1 were accumulated in the nucleus in S. pombe under nitrosative stress in a concentration and time dependent manner. Atf1 is also found to be nuclear delocalized under longer nitrosative stress.

Original language	English (US)
Pages (from-to)	82-90
Number of pages	9
Journal	Microbiological Research
Volume	206
DOIs	https://doi.org/10.1016/j.micres.2017.10.002
State	Published - Jan 2018
Externally published	Yes

Keywords

Atf1
Nitric oxide
Nitrosative stress
Nuclear localization
Schizosaccharomyces pombe
Sty1

ASJC Scopus subject areas

Microbiology

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10.1016/j.micres.2017.10.002

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@article{b9caa611ece345e7987d64466e9a2384,

title = "Transcription factors Atf1 and Sty1 promote stress tolerance under nitrosative stress in Schizosaccharomyces pombe",

abstract = "Nitric Oxide (NO) and its associated reactive nitrogen species (RNS) produce nitrosative stress under various pathophysiological conditions in eukaryotes. The fission yeast Schizosaccharomyces pombe regulates stress response mainly through the Sty1-Atf1 MAP Kinase pathway. The present study deals with the role of transcription factor Atf1 and Sty1 in S. pombe under nitrosative stress. In this study, exposure to an NO donor resulted in S-phase slowdown with associated mitotic block in S. pombe. Deletion of sty1 and atf1 in S. pombe had differential growth sensitivity towards NO donor. Both Sty1 and Atf1 were involved in regulating mitotic slowdown in S. pombe under nitrosative stress. Experimental data obtained in this study reveals a novel role of Atf1 in initiating the replication slowdown in S. pombe under nitrosative stress. Both Sty1 and Atf1 were accumulated in the nucleus in S. pombe under nitrosative stress in a concentration and time dependent manner. Atf1 is also found to be nuclear delocalized under longer nitrosative stress.",

keywords = "Atf1, Nitric oxide, Nitrosative stress, Nuclear localization, Schizosaccharomyces pombe, Sty1",

author = "Puranjoy Kar and Pranjal Biswas and Patra, {Sourav Kumar} and Sanjay Ghosh",

note = "Funding Information: We thank Dr. Sanjaya Mallick and Dr. Debajit Bhowmik (at the University of Calcutta-BD Flow cytometry facility at CRNN, University of Calcutta) for technical help. The consumable for the entire work was supported by DBT, Govt. of India, Grant BT/PR8588/BRB/10/1247/2013 Dated 24/09/2015. We thank CRNN, University of Calcutta, DST-FIST, DBT-IPLS, UPE, DAE, and UGC CAS Phase II Govt. of India for providing their infrastructural facility, UGC, Govt. of India, Grant no. UGC/722/Jr. Fellow (Sc) dated 23.04.2014 for providing fellowship to P.K., CSIR, Govt. of India, Grant no. 09/028 (0809)/2010-EMR-I dated 10.02.2015 for providing fellowship to P.B. The funders had no role in the study, design, data collection and analysis, decision to publish, or preparation of the manuscript. Publisher Copyright: {\textcopyright} 2017 Elsevier GmbH",

year = "2018",

month = jan,

doi = "10.1016/j.micres.2017.10.002",

language = "English (US)",

volume = "206",

pages = "82--90",

journal = "Microbiological Research",

issn = "0944-5013",

publisher = "Urban und Fischer Verlag Jena",

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TY - JOUR

T1 - Transcription factors Atf1 and Sty1 promote stress tolerance under nitrosative stress in Schizosaccharomyces pombe

AU - Kar, Puranjoy

AU - Biswas, Pranjal

AU - Patra, Sourav Kumar

AU - Ghosh, Sanjay

N1 - Funding Information: We thank Dr. Sanjaya Mallick and Dr. Debajit Bhowmik (at the University of Calcutta-BD Flow cytometry facility at CRNN, University of Calcutta) for technical help. The consumable for the entire work was supported by DBT, Govt. of India, Grant BT/PR8588/BRB/10/1247/2013 Dated 24/09/2015. We thank CRNN, University of Calcutta, DST-FIST, DBT-IPLS, UPE, DAE, and UGC CAS Phase II Govt. of India for providing their infrastructural facility, UGC, Govt. of India, Grant no. UGC/722/Jr. Fellow (Sc) dated 23.04.2014 for providing fellowship to P.K., CSIR, Govt. of India, Grant no. 09/028 (0809)/2010-EMR-I dated 10.02.2015 for providing fellowship to P.B. The funders had no role in the study, design, data collection and analysis, decision to publish, or preparation of the manuscript. Publisher Copyright: © 2017 Elsevier GmbH

PY - 2018/1

Y1 - 2018/1

N2 - Nitric Oxide (NO) and its associated reactive nitrogen species (RNS) produce nitrosative stress under various pathophysiological conditions in eukaryotes. The fission yeast Schizosaccharomyces pombe regulates stress response mainly through the Sty1-Atf1 MAP Kinase pathway. The present study deals with the role of transcription factor Atf1 and Sty1 in S. pombe under nitrosative stress. In this study, exposure to an NO donor resulted in S-phase slowdown with associated mitotic block in S. pombe. Deletion of sty1 and atf1 in S. pombe had differential growth sensitivity towards NO donor. Both Sty1 and Atf1 were involved in regulating mitotic slowdown in S. pombe under nitrosative stress. Experimental data obtained in this study reveals a novel role of Atf1 in initiating the replication slowdown in S. pombe under nitrosative stress. Both Sty1 and Atf1 were accumulated in the nucleus in S. pombe under nitrosative stress in a concentration and time dependent manner. Atf1 is also found to be nuclear delocalized under longer nitrosative stress.

AB - Nitric Oxide (NO) and its associated reactive nitrogen species (RNS) produce nitrosative stress under various pathophysiological conditions in eukaryotes. The fission yeast Schizosaccharomyces pombe regulates stress response mainly through the Sty1-Atf1 MAP Kinase pathway. The present study deals with the role of transcription factor Atf1 and Sty1 in S. pombe under nitrosative stress. In this study, exposure to an NO donor resulted in S-phase slowdown with associated mitotic block in S. pombe. Deletion of sty1 and atf1 in S. pombe had differential growth sensitivity towards NO donor. Both Sty1 and Atf1 were involved in regulating mitotic slowdown in S. pombe under nitrosative stress. Experimental data obtained in this study reveals a novel role of Atf1 in initiating the replication slowdown in S. pombe under nitrosative stress. Both Sty1 and Atf1 were accumulated in the nucleus in S. pombe under nitrosative stress in a concentration and time dependent manner. Atf1 is also found to be nuclear delocalized under longer nitrosative stress.

KW - Atf1

KW - Nitric oxide

KW - Nitrosative stress

KW - Nuclear localization

KW - Schizosaccharomyces pombe

KW - Sty1

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U2 - 10.1016/j.micres.2017.10.002

DO - 10.1016/j.micres.2017.10.002

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C2 - 29146263

AN - SCOPUS:85031758992

SN - 0944-5013

VL - 206

SP - 82

EP - 90

JO - Microbiological Research

JF - Microbiological Research

ER -

Transcription factors Atf1 and Sty1 promote stress tolerance under nitrosative stress in Schizosaccharomyces pombe

Abstract

Keywords

ASJC Scopus subject areas

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