Transcriptional activation of cellular oneogenes fos, jun, and myc by human cytomegalovirus

Istvan Boldogh, S. Abubakar, C. Z. Deng, T. Albrecht

Research output: Contribution to journalArticle

84 Citations (Scopus)

Abstract

The mechanisms responsible for the human cytomegalovirus (HCMV)-induced increase in cellular oncogene RNAs for c-jun, c-fos, and c-myc in human embryo lung cells (I. Boldogh, S. AbuBakar, and T. Albrecht, Science 247:561-564, 1990) were investigated. Results of transcription assays indicated that the rapid increase in RNA levels for the above-noted oncogenes was controlled at the transcriptional level and was related to enhanced transcription. The maximum rates of transcription for c-jun and c-fos genes occurred at 40 min postinfection, while for the c-myc gene the maximum rate occurred at about 60 min. The magnitude of HCMV-induced activation of these cellular genes was similar to the activation induced by serum. The half-lives of the cellular oncogenes showed similar decay rates after either serum or HCMV activation when measured by dactinomycin chase. The half-life for c-fos or c-jun was about 20 min, and that for c-myc was about 40 min. Furthermore, inhibition of the RNA increase by dactinomycin or by α-amanitin suggested that the increase in RNA levels was due to an increase in the transcriptional activity of oncogenes triggered by HCMV.

Original languageEnglish (US)
Pages (from-to)1568-1571
Number of pages4
JournalJournal of Virology
Volume65
Issue number3
StatePublished - 1991

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Human herpesvirus 5
oncogenes
transcriptional activation
Cytomegalovirus
Transcriptional Activation
Oncogenes
RNA
actinomycin D
transcription (genetics)
half life
Dactinomycin
amanitins
genes
Amanitins
fos Genes
myc Genes
embryo (animal)
Serum
lungs
deterioration

ASJC Scopus subject areas

  • Immunology

Cite this

Transcriptional activation of cellular oneogenes fos, jun, and myc by human cytomegalovirus. / Boldogh, Istvan; Abubakar, S.; Deng, C. Z.; Albrecht, T.

In: Journal of Virology, Vol. 65, No. 3, 1991, p. 1568-1571.

Research output: Contribution to journalArticle

Boldogh, I, Abubakar, S, Deng, CZ & Albrecht, T 1991, 'Transcriptional activation of cellular oneogenes fos, jun, and myc by human cytomegalovirus', Journal of Virology, vol. 65, no. 3, pp. 1568-1571.
Boldogh, Istvan ; Abubakar, S. ; Deng, C. Z. ; Albrecht, T. / Transcriptional activation of cellular oneogenes fos, jun, and myc by human cytomegalovirus. In: Journal of Virology. 1991 ; Vol. 65, No. 3. pp. 1568-1571.
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