Transcriptional and physiological characterization of Escherichia coli K12 MG1655 grown under low shear simulated microgravity for 1000 generation

Fathi Karouia, Madhan T. Tirumalai, Mayra A. Nelman-Gonzalez, Clarence F. Sams, Mark C. Ott, Duane L. Pierson, Yuriy Fofanov, Richard C. Willson, George E. Fox

Research output: Chapter in Book/Report/Conference proceedingConference contribution

Abstract

As the duration of spaceflight missions increases, it becomes important to evaluate the long-term effects of microgravity on bacteria. For the first time, we have grown Escherichia coli K-12 (E. coli) strain MG1655 for up to a thousand generations under simulated microgravity conditions (1000G) using an antibiotic based sterilization protocol between cycles. The long-term adapted cells were examined by scanning electron microscopy and subjected to various antibiotic and chemical stresses in order to assess stress response. In addition, molecular studies identified significant changes in gene expression relative to a short-term adapted control and established that multiple mutations had occurred. The results provide significant evidence that the initial environmental response to microgravity seen in short-term studies is in fact replaced by a different long-term adaptation that is based at least in part on genomic changes. The protein Hfq did not appear to be involved in the adaptation. A portion of the long-term adaptation related to antibiotic resistance, may in part be a response to the sterilization protocol used.

Original languageEnglish (US)
Title of host publication61st International Astronautical Congress 2010, IAC 2010
Pages9086-9089
Number of pages4
StatePublished - Dec 1 2010
Event61st International Astronautical Congress 2010, IAC 2010 - Prague, Czech Republic
Duration: Sep 27 2010Oct 1 2010

Publication series

Name61st International Astronautical Congress 2010, IAC 2010
Volume11

Other

Other61st International Astronautical Congress 2010, IAC 2010
CountryCzech Republic
CityPrague
Period9/27/1010/1/10

ASJC Scopus subject areas

  • Aerospace Engineering
  • Astronomy and Astrophysics

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    Karouia, F., Tirumalai, M. T., Nelman-Gonzalez, M. A., Sams, C. F., Ott, M. C., Pierson, D. L., Fofanov, Y., Willson, R. C., & Fox, G. E. (2010). Transcriptional and physiological characterization of Escherichia coli K12 MG1655 grown under low shear simulated microgravity for 1000 generation. In 61st International Astronautical Congress 2010, IAC 2010 (pp. 9086-9089). (61st International Astronautical Congress 2010, IAC 2010; Vol. 11).