Transcriptional and physiological characterization of Escherichia coli K12 MG1655 grown under low shear simulated microgravity for 1000 generation

Fathi Karouia, Madhan T. Tirumalai, Mayra A. Nelman-Gonzalez, Clarence F. Sams, Mark C. Ott, Duane L. Pierson, Yuriy Fofanov, Richard C. Willson, George E. Fox

Research output: Chapter in Book/Report/Conference proceedingConference contribution

Abstract

As the duration of spaceflight missions increases, it becomes important to evaluate the long-term effects of microgravity on bacteria. For the first time, we have grown Escherichia coli K-12 (E. coli) strain MG1655 for up to a thousand generations under simulated microgravity conditions (1000G) using an antibiotic based sterilization protocol between cycles. The long-term adapted cells were examined by scanning electron microscopy and subjected to various antibiotic and chemical stresses in order to assess stress response. In addition, molecular studies identified significant changes in gene expression relative to a short-term adapted control and established that multiple mutations had occurred. The results provide significant evidence that the initial environmental response to microgravity seen in short-term studies is in fact replaced by a different long-term adaptation that is based at least in part on genomic changes. The protein Hfq did not appear to be involved in the adaptation. A portion of the long-term adaptation related to antibiotic resistance, may in part be a response to the sterilization protocol used.

Original languageEnglish (US)
Title of host publication61st International Astronautical Congress 2010, IAC 2010
Pages9086-9089
Number of pages4
Volume11
StatePublished - 2010
Externally publishedYes
Event61st International Astronautical Congress 2010, IAC 2010 - Prague, Czech Republic
Duration: Sep 27 2010Oct 1 2010

Other

Other61st International Astronautical Congress 2010, IAC 2010
CountryCzech Republic
CityPrague
Period9/27/1010/1/10

Fingerprint

antibiotics
Microgravity
Antibiotics
Escherichia
microgravity
Escherichia coli
shear
long term effects
gene expression
mutations
Gene expression
bacteria
Bacteria
proteins
Proteins
Scanning electron microscopy
cycles
scanning electron microscopy
cells

ASJC Scopus subject areas

  • Aerospace Engineering
  • Astronomy and Astrophysics

Cite this

Karouia, F., Tirumalai, M. T., Nelman-Gonzalez, M. A., Sams, C. F., Ott, M. C., Pierson, D. L., ... Fox, G. E. (2010). Transcriptional and physiological characterization of Escherichia coli K12 MG1655 grown under low shear simulated microgravity for 1000 generation. In 61st International Astronautical Congress 2010, IAC 2010 (Vol. 11, pp. 9086-9089)

Transcriptional and physiological characterization of Escherichia coli K12 MG1655 grown under low shear simulated microgravity for 1000 generation. / Karouia, Fathi; Tirumalai, Madhan T.; Nelman-Gonzalez, Mayra A.; Sams, Clarence F.; Ott, Mark C.; Pierson, Duane L.; Fofanov, Yuriy; Willson, Richard C.; Fox, George E.

61st International Astronautical Congress 2010, IAC 2010. Vol. 11 2010. p. 9086-9089.

Research output: Chapter in Book/Report/Conference proceedingConference contribution

Karouia, F, Tirumalai, MT, Nelman-Gonzalez, MA, Sams, CF, Ott, MC, Pierson, DL, Fofanov, Y, Willson, RC & Fox, GE 2010, Transcriptional and physiological characterization of Escherichia coli K12 MG1655 grown under low shear simulated microgravity for 1000 generation. in 61st International Astronautical Congress 2010, IAC 2010. vol. 11, pp. 9086-9089, 61st International Astronautical Congress 2010, IAC 2010, Prague, Czech Republic, 9/27/10.
Karouia F, Tirumalai MT, Nelman-Gonzalez MA, Sams CF, Ott MC, Pierson DL et al. Transcriptional and physiological characterization of Escherichia coli K12 MG1655 grown under low shear simulated microgravity for 1000 generation. In 61st International Astronautical Congress 2010, IAC 2010. Vol. 11. 2010. p. 9086-9089
Karouia, Fathi ; Tirumalai, Madhan T. ; Nelman-Gonzalez, Mayra A. ; Sams, Clarence F. ; Ott, Mark C. ; Pierson, Duane L. ; Fofanov, Yuriy ; Willson, Richard C. ; Fox, George E. / Transcriptional and physiological characterization of Escherichia coli K12 MG1655 grown under low shear simulated microgravity for 1000 generation. 61st International Astronautical Congress 2010, IAC 2010. Vol. 11 2010. pp. 9086-9089
@inproceedings{1ddd972ea00c49458380ce04d59b86f0,
title = "Transcriptional and physiological characterization of Escherichia coli K12 MG1655 grown under low shear simulated microgravity for 1000 generation",
abstract = "As the duration of spaceflight missions increases, it becomes important to evaluate the long-term effects of microgravity on bacteria. For the first time, we have grown Escherichia coli K-12 (E. coli) strain MG1655 for up to a thousand generations under simulated microgravity conditions (1000G) using an antibiotic based sterilization protocol between cycles. The long-term adapted cells were examined by scanning electron microscopy and subjected to various antibiotic and chemical stresses in order to assess stress response. In addition, molecular studies identified significant changes in gene expression relative to a short-term adapted control and established that multiple mutations had occurred. The results provide significant evidence that the initial environmental response to microgravity seen in short-term studies is in fact replaced by a different long-term adaptation that is based at least in part on genomic changes. The protein Hfq did not appear to be involved in the adaptation. A portion of the long-term adaptation related to antibiotic resistance, may in part be a response to the sterilization protocol used.",
author = "Fathi Karouia and Tirumalai, {Madhan T.} and Nelman-Gonzalez, {Mayra A.} and Sams, {Clarence F.} and Ott, {Mark C.} and Pierson, {Duane L.} and Yuriy Fofanov and Willson, {Richard C.} and Fox, {George E.}",
year = "2010",
language = "English (US)",
isbn = "9781617823688",
volume = "11",
pages = "9086--9089",
booktitle = "61st International Astronautical Congress 2010, IAC 2010",

}

TY - GEN

T1 - Transcriptional and physiological characterization of Escherichia coli K12 MG1655 grown under low shear simulated microgravity for 1000 generation

AU - Karouia, Fathi

AU - Tirumalai, Madhan T.

AU - Nelman-Gonzalez, Mayra A.

AU - Sams, Clarence F.

AU - Ott, Mark C.

AU - Pierson, Duane L.

AU - Fofanov, Yuriy

AU - Willson, Richard C.

AU - Fox, George E.

PY - 2010

Y1 - 2010

N2 - As the duration of spaceflight missions increases, it becomes important to evaluate the long-term effects of microgravity on bacteria. For the first time, we have grown Escherichia coli K-12 (E. coli) strain MG1655 for up to a thousand generations under simulated microgravity conditions (1000G) using an antibiotic based sterilization protocol between cycles. The long-term adapted cells were examined by scanning electron microscopy and subjected to various antibiotic and chemical stresses in order to assess stress response. In addition, molecular studies identified significant changes in gene expression relative to a short-term adapted control and established that multiple mutations had occurred. The results provide significant evidence that the initial environmental response to microgravity seen in short-term studies is in fact replaced by a different long-term adaptation that is based at least in part on genomic changes. The protein Hfq did not appear to be involved in the adaptation. A portion of the long-term adaptation related to antibiotic resistance, may in part be a response to the sterilization protocol used.

AB - As the duration of spaceflight missions increases, it becomes important to evaluate the long-term effects of microgravity on bacteria. For the first time, we have grown Escherichia coli K-12 (E. coli) strain MG1655 for up to a thousand generations under simulated microgravity conditions (1000G) using an antibiotic based sterilization protocol between cycles. The long-term adapted cells were examined by scanning electron microscopy and subjected to various antibiotic and chemical stresses in order to assess stress response. In addition, molecular studies identified significant changes in gene expression relative to a short-term adapted control and established that multiple mutations had occurred. The results provide significant evidence that the initial environmental response to microgravity seen in short-term studies is in fact replaced by a different long-term adaptation that is based at least in part on genomic changes. The protein Hfq did not appear to be involved in the adaptation. A portion of the long-term adaptation related to antibiotic resistance, may in part be a response to the sterilization protocol used.

UR - http://www.scopus.com/inward/record.url?scp=79959480451&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=79959480451&partnerID=8YFLogxK

M3 - Conference contribution

AN - SCOPUS:79959480451

SN - 9781617823688

VL - 11

SP - 9086

EP - 9089

BT - 61st International Astronautical Congress 2010, IAC 2010

ER -