Transcriptional Profiling of mRNA Expression in the Mouse Distal Colon

Willemijntje A. Hoogerwerf, Mala Sinha, Ana Conesa, Bruce A. Luxon, Vahakn B. Shahinian, Germaine Cornélissen, Franz Halberg, Jonathon Bostwick, John Timm, Vincent M. Cassone

    Research output: Contribution to journalArticle

    64 Citations (Scopus)

    Abstract

    Background & Aims: Intestinal epithelial cells and the myenteric plexus of the mouse gastrointestinal tract contain a circadian clock-based intrinsic time-keeping system. Because disruption of the biological clock has been associated with increased susceptibility to colon cancer and gastrointestinal symptoms, we aimed to identify rhythmically expressed genes in the mouse distal colon. Methods: Microarray analysis was used to identify genes that were rhythmically expressed over a 24-hour light/dark cycle. The transcripts were then classified according to expression pattern, function, and association with physiologic and pathophysiologic processes of the colon. Results: A circadian gene expression pattern was detected in approximately 3.7% of distal colonic genes. A large percentage of these genes were involved in cell signaling, differentiation, and proliferation and cell death. Of all the rhythmically expressed genes in the mouse colon, approximately 7% (64/906) have been associated with colorectal cancer formation (eg, B-cell leukemia/lymphoma-2 [Bcl2]) and 1.8% (18/906) with various colonic functions such as motility and secretion (eg, vasoactive intestinal polypeptide, cystic fibrosis transmembrane conductance regulator). Conclusions: A subset of genes in the murine colon follows a rhythmic expression pattern. These findings may have significant implications for colonic physiology and pathophysiology.

    Original languageEnglish (US)
    Pages (from-to)2019-2029
    Number of pages11
    JournalGastroenterology
    Volume135
    Issue number6
    DOIs
    StatePublished - Dec 2008

    Fingerprint

    Colon
    Messenger RNA
    Genes
    B-Cell Leukemia
    Biological Clocks
    Myenteric Plexus
    Cystic Fibrosis Transmembrane Conductance Regulator
    Circadian Clocks
    Vasoactive Intestinal Peptide
    Photoperiod
    B-Cell Lymphoma
    Microarray Analysis
    Colonic Neoplasms
    Gastrointestinal Tract
    Cell Differentiation
    Colorectal Neoplasms
    Cell Death
    Epithelial Cells
    Cell Proliferation
    Gene Expression

    ASJC Scopus subject areas

    • Gastroenterology

    Cite this

    Hoogerwerf, W. A., Sinha, M., Conesa, A., Luxon, B. A., Shahinian, V. B., Cornélissen, G., ... Cassone, V. M. (2008). Transcriptional Profiling of mRNA Expression in the Mouse Distal Colon. Gastroenterology, 135(6), 2019-2029. https://doi.org/10.1053/j.gastro.2008.08.048

    Transcriptional Profiling of mRNA Expression in the Mouse Distal Colon. / Hoogerwerf, Willemijntje A.; Sinha, Mala; Conesa, Ana; Luxon, Bruce A.; Shahinian, Vahakn B.; Cornélissen, Germaine; Halberg, Franz; Bostwick, Jonathon; Timm, John; Cassone, Vincent M.

    In: Gastroenterology, Vol. 135, No. 6, 12.2008, p. 2019-2029.

    Research output: Contribution to journalArticle

    Hoogerwerf, WA, Sinha, M, Conesa, A, Luxon, BA, Shahinian, VB, Cornélissen, G, Halberg, F, Bostwick, J, Timm, J & Cassone, VM 2008, 'Transcriptional Profiling of mRNA Expression in the Mouse Distal Colon', Gastroenterology, vol. 135, no. 6, pp. 2019-2029. https://doi.org/10.1053/j.gastro.2008.08.048
    Hoogerwerf WA, Sinha M, Conesa A, Luxon BA, Shahinian VB, Cornélissen G et al. Transcriptional Profiling of mRNA Expression in the Mouse Distal Colon. Gastroenterology. 2008 Dec;135(6):2019-2029. https://doi.org/10.1053/j.gastro.2008.08.048
    Hoogerwerf, Willemijntje A. ; Sinha, Mala ; Conesa, Ana ; Luxon, Bruce A. ; Shahinian, Vahakn B. ; Cornélissen, Germaine ; Halberg, Franz ; Bostwick, Jonathon ; Timm, John ; Cassone, Vincent M. / Transcriptional Profiling of mRNA Expression in the Mouse Distal Colon. In: Gastroenterology. 2008 ; Vol. 135, No. 6. pp. 2019-2029.
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    AU - Luxon, Bruce A.

    AU - Shahinian, Vahakn B.

    AU - Cornélissen, Germaine

    AU - Halberg, Franz

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