Transcriptional profiling of spinal cord injury-induced central neuropathic pain

Olivera Nesic, Julieann Lee, Kathia M. Johnson, Zaiming Ye, Guo Ying Xu, Geda C. Unabia, Thomas Wood, David J. McAdoo, Karin N. Westlund, Claire E. Hulsebosch, J. Regino Perez-Polo

Research output: Contribution to journalArticle

108 Citations (Scopus)

Abstract

Central neuropathic pain (CNP) is an important problem following spinal cord injury (SCI), because it severely affects the quality of life of SCI patients. As in the patient population, the majority of rats develop significant allodynia (CNP rats) after moderate SCI. However, about 10% of SCI rats do not develop allodynia, or develop significantly less allodynia than CNP rats (non-CNP rats). To identify transcriptional changes underlying CNP development after SCI, we used Affymetrix DNA microarrays and RNAs extracted from the spinal cords of CNP and non-CNP rats. DNA microarry analysis showed significantly increased expression of a number of genes associated with inflammation and astrocytic activation in the spinal cords of rats that developed CNP. For example, mRNA levels of glial fibrilary acidic protein (GFAP) and Aquaporin 4 (AQP4) significantly increased in CNP rats. We also found that GFAP, S100β and AQP4 protein elevation persisted for at least 9 months throughout contused spinal cords, consistent with the chronic nature of CNP. Thus, we hypothesize that CNP development results, in part, from dysfunctional, chronically "over-activated" astrocytes. Although, it has been shown that activated astrocytes are associated with peripheral neuropathic pain, this has not previously been demonstrated in CNP after SCI.

Original languageEnglish (US)
Pages (from-to)998-1014
Number of pages17
JournalJournal of Neurochemistry
Volume95
Issue number4
DOIs
StatePublished - Nov 2005

Fingerprint

Neuralgia
Spinal Cord Injuries
Rats
Aquaporin 4
Hyperalgesia
Spinal Cord
Neuroglia
Astrocytes
S100 Proteins
DNA
Microarrays
Contusions
Genes
Chemical activation
RNA
Oligonucleotide Array Sequence Analysis
Messenger RNA
Quality of Life

Keywords

  • Astrocytes
  • DNA microarrays
  • Glial fibrilary acidic protein
  • Inflammation
  • Pain
  • Spinal cord injury

ASJC Scopus subject areas

  • Biochemistry
  • Cellular and Molecular Neuroscience

Cite this

Nesic, O., Lee, J., Johnson, K. M., Ye, Z., Xu, G. Y., Unabia, G. C., ... Perez-Polo, J. R. (2005). Transcriptional profiling of spinal cord injury-induced central neuropathic pain. Journal of Neurochemistry, 95(4), 998-1014. https://doi.org/10.1111/j.1471-4159.2005.03462.x

Transcriptional profiling of spinal cord injury-induced central neuropathic pain. / Nesic, Olivera; Lee, Julieann; Johnson, Kathia M.; Ye, Zaiming; Xu, Guo Ying; Unabia, Geda C.; Wood, Thomas; McAdoo, David J.; Westlund, Karin N.; Hulsebosch, Claire E.; Perez-Polo, J. Regino.

In: Journal of Neurochemistry, Vol. 95, No. 4, 11.2005, p. 998-1014.

Research output: Contribution to journalArticle

Nesic, O, Lee, J, Johnson, KM, Ye, Z, Xu, GY, Unabia, GC, Wood, T, McAdoo, DJ, Westlund, KN, Hulsebosch, CE & Perez-Polo, JR 2005, 'Transcriptional profiling of spinal cord injury-induced central neuropathic pain', Journal of Neurochemistry, vol. 95, no. 4, pp. 998-1014. https://doi.org/10.1111/j.1471-4159.2005.03462.x
Nesic, Olivera ; Lee, Julieann ; Johnson, Kathia M. ; Ye, Zaiming ; Xu, Guo Ying ; Unabia, Geda C. ; Wood, Thomas ; McAdoo, David J. ; Westlund, Karin N. ; Hulsebosch, Claire E. ; Perez-Polo, J. Regino. / Transcriptional profiling of spinal cord injury-induced central neuropathic pain. In: Journal of Neurochemistry. 2005 ; Vol. 95, No. 4. pp. 998-1014.
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