Transcriptome analysis revealed the synergism of novel rhodethrin inhibition on biofilm architecture, antibiotic resistance and quorum sensing in Enterococcus faecalis

Eswar Rao Tatta, Sayan Paul, Ranjith Kumavath

Research output: Contribution to journalArticlepeer-review

2 Scopus citations

Abstract

Enterococcus sp. emerged as an opportunistic nosocomial pathogen with the highest antibiotic resistance and mortality rate. Biofilm is problematic primarily since it is regulated by the global bacterial cell to cell communication mediated by the quorum sensing signaling system. Thus, potential natural antagonists in a novel drug formulation against biofilm-forming Enterococcus faecalis is critical. We used RNA-Seq to evaluate the effects of the novel molecule rhodethrin with chloramphenicol induced on Enterococcus faecalis and DEGs were identified. In transcriptome sequence analysis, a total of 448 with control Vs rhodethrin, 1591 were in control Vs chloramphenicol, 379 genes were DEGs from control Vs synergies, in rhodethrin with chloramphenicol, 379 genes were differentially expressed, whereas 264 genes were significantly downregulated, indicating that 69.69% of E. faecalis was altered. The transcriptional sequence data further expression analysis qRT-PCR, and the results shed that the expression profiles of five significant biofilm formation responsible genes such as, Ace, AtpB, lepA, bopD, and typA, 3 genes involved in quorum sensing are sylA, fsrC and camE, and 4 genes involved in resistance were among including liaX, typA, EfrA, and lepA, were significantly suppressed expressions of the biofilm, quorum sensing, and resistance that are supported by transcriptome analysis.

Original languageEnglish (US)
Article number147436
JournalGene
Volume871
DOIs
StatePublished - Jun 30 2023
Externally publishedYes

Keywords

  • Anti biofilm
  • Anti-quorum sensing
  • Antibiotic resistance
  • Differentially expressed genes
  • Enterococcus faecalis
  • Synergistic
  • Transcriptome
  • chloramphenicol
  • rhodethrin

ASJC Scopus subject areas

  • Genetics

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