Transcriptomic and innate immune responses to Yersinia pestis in the lymph node during bubonic plague

Jason Comer, Daniel E. Sturdevant, Aaron B. Carmody, Kimmo Virtaneva, Donald Gardner, Dan Long, Rebecca Rosenke, Stephen F. Porcella, B. Joseph Hinnebusch

Research output: Contribution to journalArticle

35 Citations (Scopus)

Abstract

A delayed inflammatory response is a prominent feature of infection with Yersinia pestis, the agent of bubonic and pneumonic plague. Using a rat model of bubonic plague, we examined lymph node histopathology, transcriptome, and extracellular cytokine levels to broadly characterize the kinetics and extent of the host response to Y. pestis and how it is influenced by the Yersinia virulence plasmid (pYV). Remarkably, dissemination and multiplication of wild-type Y. pestis during the bubonic stage of disease did not induce any detectable gene expression or cytokine response by host lymph node cells in the developing bubo. Only after systemic spread had led to terminal septicemic plague was a transcriptomic response detected, which included upregulation of several cytokine, chemokine, and other immune response genes. Although an initial intracellular phase of Y. pestis infection has been postulated, a Th1-type cytokine response associated with classical activation of macrophages was not observed during the bubonic stage of disease. However, elevated levels of interleukin-17 (IL-17) were present in infected lymph nodes. In the absence of pYV, sustained recruitment to the lymph node of polymorphonuclear leukocytes (PMN, or neutrophils), the major IL-17 effector cells, correlated with clearance of infection. Thus, the ability to counteract a PMN response in the lymph node appears to be a major in vivo function of the Y. pestis virulence plasmid.

Original languageEnglish (US)
Pages (from-to)5086-5098
Number of pages13
JournalInfection and immunity
Volume78
Issue number12
DOIs
StatePublished - Dec 1 2010
Externally publishedYes

Fingerprint

Yersinia pestis
Plague
Innate Immunity
Lymph Nodes
Cytokines
Interleukin-17
Virulence
Neutrophils
Plasmids
Yersinia Infections
Yersinia
Macrophage Activation
Infection
Transcriptome
Chemokines
Up-Regulation
Gene Expression
Genes

ASJC Scopus subject areas

  • Parasitology
  • Microbiology
  • Immunology
  • Infectious Diseases

Cite this

Comer, J., Sturdevant, D. E., Carmody, A. B., Virtaneva, K., Gardner, D., Long, D., ... Hinnebusch, B. J. (2010). Transcriptomic and innate immune responses to Yersinia pestis in the lymph node during bubonic plague. Infection and immunity, 78(12), 5086-5098. https://doi.org/10.1128/IAI.00256-10

Transcriptomic and innate immune responses to Yersinia pestis in the lymph node during bubonic plague. / Comer, Jason; Sturdevant, Daniel E.; Carmody, Aaron B.; Virtaneva, Kimmo; Gardner, Donald; Long, Dan; Rosenke, Rebecca; Porcella, Stephen F.; Hinnebusch, B. Joseph.

In: Infection and immunity, Vol. 78, No. 12, 01.12.2010, p. 5086-5098.

Research output: Contribution to journalArticle

Comer, J, Sturdevant, DE, Carmody, AB, Virtaneva, K, Gardner, D, Long, D, Rosenke, R, Porcella, SF & Hinnebusch, BJ 2010, 'Transcriptomic and innate immune responses to Yersinia pestis in the lymph node during bubonic plague', Infection and immunity, vol. 78, no. 12, pp. 5086-5098. https://doi.org/10.1128/IAI.00256-10
Comer J, Sturdevant DE, Carmody AB, Virtaneva K, Gardner D, Long D et al. Transcriptomic and innate immune responses to Yersinia pestis in the lymph node during bubonic plague. Infection and immunity. 2010 Dec 1;78(12):5086-5098. https://doi.org/10.1128/IAI.00256-10
Comer, Jason ; Sturdevant, Daniel E. ; Carmody, Aaron B. ; Virtaneva, Kimmo ; Gardner, Donald ; Long, Dan ; Rosenke, Rebecca ; Porcella, Stephen F. ; Hinnebusch, B. Joseph. / Transcriptomic and innate immune responses to Yersinia pestis in the lymph node during bubonic plague. In: Infection and immunity. 2010 ; Vol. 78, No. 12. pp. 5086-5098.
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