Transfection-independent production of alphavirus replicon particles based on poxvirus expression vectors

Nikos Vasilakis, Darlene Falvey, Seema S. Gangolli, John Coleman, Jacek Kowalski, Stephen A. Udem, Timothy J. Zamb, Gerald R. Kovacs

Research output: Contribution to journalArticlepeer-review

15 Scopus citations


This report describes a transfection-independent system for packaging alphavirus replicon vectors using modified vaccinia virus Ankara (MVA) vectors to express all of the RNA components necessary for the production of Venezuelan equine encephalitis (VEE) virus replicon particles (VRP). Infection of mammalian cells with these recombinant MVA vectors resulted in robust expression of VEE structural genes, replication of the alphavirus vector and high titers of VRP. In addition, VRP packaging was achieved in a cell type (fetal rhesus lung) that has been approved for the manufacturing of vaccines destined for human use.

Original languageEnglish (US)
Pages (from-to)932-935
Number of pages4
JournalNature Biotechnology
Issue number8
StatePublished - Aug 1 2003

ASJC Scopus subject areas

  • Biotechnology
  • Bioengineering
  • Biomedical Engineering
  • Applied Microbiology and Biotechnology
  • Molecular Medicine


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