Transfection-independent production of alphavirus replicon particles based on poxvirus expression vectors

Nikos Vasilakis, Darlene Falvey, Seema S. Gangolli, John Coleman, Jacek Kowalski, Stephen A. Udem, Timothy J. Zamb, Gerald R. Kovacs

Research output: Contribution to journalArticle

13 Citations (Scopus)

Abstract

This report describes a transfection-independent system for packaging alphavirus replicon vectors using modified vaccinia virus Ankara (MVA) vectors to express all of the RNA components necessary for the production of Venezuelan equine encephalitis (VEE) virus replicon particles (VRP). Infection of mammalian cells with these recombinant MVA vectors resulted in robust expression of VEE structural genes, replication of the alphavirus vector and high titers of VRP. In addition, VRP packaging was achieved in a cell type (fetal rhesus lung) that has been approved for the manufacturing of vaccines destined for human use.

Original languageEnglish (US)
Pages (from-to)932-935
Number of pages4
JournalNature Biotechnology
Volume21
Issue number8
DOIs
StatePublished - Aug 1 2003

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Alphavirus
Poxviridae
Replicon
Viruses
Transfection
Virion
Vaccinia virus
Product Packaging
Venezuelan Equine Encephalomyelitides
Venezuelan Equine Encephalitis Viruses
Packaging
Vaccines
RNA
Lung
Genes
Cells
Infection

ASJC Scopus subject areas

  • Microbiology

Cite this

Vasilakis, N., Falvey, D., Gangolli, S. S., Coleman, J., Kowalski, J., Udem, S. A., ... Kovacs, G. R. (2003). Transfection-independent production of alphavirus replicon particles based on poxvirus expression vectors. Nature Biotechnology, 21(8), 932-935. https://doi.org/10.1038/nbt845

Transfection-independent production of alphavirus replicon particles based on poxvirus expression vectors. / Vasilakis, Nikos; Falvey, Darlene; Gangolli, Seema S.; Coleman, John; Kowalski, Jacek; Udem, Stephen A.; Zamb, Timothy J.; Kovacs, Gerald R.

In: Nature Biotechnology, Vol. 21, No. 8, 01.08.2003, p. 932-935.

Research output: Contribution to journalArticle

Vasilakis, N, Falvey, D, Gangolli, SS, Coleman, J, Kowalski, J, Udem, SA, Zamb, TJ & Kovacs, GR 2003, 'Transfection-independent production of alphavirus replicon particles based on poxvirus expression vectors', Nature Biotechnology, vol. 21, no. 8, pp. 932-935. https://doi.org/10.1038/nbt845
Vasilakis, Nikos ; Falvey, Darlene ; Gangolli, Seema S. ; Coleman, John ; Kowalski, Jacek ; Udem, Stephen A. ; Zamb, Timothy J. ; Kovacs, Gerald R. / Transfection-independent production of alphavirus replicon particles based on poxvirus expression vectors. In: Nature Biotechnology. 2003 ; Vol. 21, No. 8. pp. 932-935.
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