Transfer of methadone across the dually perfused preterm human placental lobule

Tatiana Nanovskaya, Ilona A. Nekhayeva, Gary Hankins, Mahmoud Ahmed

Research output: Contribution to journalArticle

47 Citations (Scopus)

Abstract

Objective: The objective of the study was to determine the effect of gestational age and P-glycoprotein expression on transplacental transfer of methadone. Study Design: Dual perfusion of placental lobule was utilized. Methadone (200 ng/mL) and its [3H]-isotope were cotransfused from the maternal-to-fetal circuit with the marker compound antipyrine (20 μg/mL) and its [14C]-isotope. Concentration of the drugs in trophoblast tissue and both circuits was determined by liquid scintillation spectrometry. Results: Fetal transfer rate of methadone in preterm placentas was 19 ± 5.8%, and in term placentas it was 31 ± 9.7% (P < .01). Clearance index of methadone in preterm placentas (0.57 ± 0.2) was lower than in term placentas (0.95 ± 0.3, P < .01). P-glycoprotein expression was higher in preterm than term placentas. Conclusion: The ex vivo transfer of methadone across preterm placentas is 30% lower than in term placentas.

Original languageEnglish (US)
JournalAmerican Journal of Obstetrics and Gynecology
Volume198
Issue number1
DOIs
StatePublished - Jan 2008

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Methadone
Placenta
P-Glycoprotein
Isotopes
Antipyrine
Trophoblasts
Gestational Age
Spectrum Analysis
Perfusion
Mothers
Pharmaceutical Preparations

Keywords

  • dual perfusion
  • methadone
  • pregnancy
  • preterm placenta

ASJC Scopus subject areas

  • Medicine(all)
  • Obstetrics and Gynecology

Cite this

Transfer of methadone across the dually perfused preterm human placental lobule. / Nanovskaya, Tatiana; Nekhayeva, Ilona A.; Hankins, Gary; Ahmed, Mahmoud.

In: American Journal of Obstetrics and Gynecology, Vol. 198, No. 1, 01.2008.

Research output: Contribution to journalArticle

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N2 - Objective: The objective of the study was to determine the effect of gestational age and P-glycoprotein expression on transplacental transfer of methadone. Study Design: Dual perfusion of placental lobule was utilized. Methadone (200 ng/mL) and its [3H]-isotope were cotransfused from the maternal-to-fetal circuit with the marker compound antipyrine (20 μg/mL) and its [14C]-isotope. Concentration of the drugs in trophoblast tissue and both circuits was determined by liquid scintillation spectrometry. Results: Fetal transfer rate of methadone in preterm placentas was 19 ± 5.8%, and in term placentas it was 31 ± 9.7% (P < .01). Clearance index of methadone in preterm placentas (0.57 ± 0.2) was lower than in term placentas (0.95 ± 0.3, P < .01). P-glycoprotein expression was higher in preterm than term placentas. Conclusion: The ex vivo transfer of methadone across preterm placentas is 30% lower than in term placentas.

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