Transfer of platelet-derived growth factor-BB gene by gene gun increases contraction of collagen lattice by fibroblasts in diabetic and non-diabetic human skin

H. Kuwahara, A. T. Mitchell, M. D. Macklin, J. Zhao, D. Listengarten, Linda Phillips

Research output: Contribution to journalArticle

4 Citations (Scopus)

Abstract

We have used an in vitro model of wound contraction, the fibroblast-populated collagen lattice, to examine the effect of platelet-derived growth factor BB (PDGF-BB) and PDGF-BB gene transfer by gene gun on the contraction of lattices composed of either diabetic or non-diabetic human fibroblasts. The area of collagen lattice and DNA synthesis were measured in 12 specimens. There were significant increases in lattice contraction with increasing doses of PDGF-BB and fibroblasts transfected with the PDGF-BB gene compared with control (p < 0.01). DNA synthesis of the non-diabetic and diabetic fibroblast lattices showed significantly increased incorporation of tritiated thymidine with increasing doses of PDGF-BB and fibroblasts transfected with the PDGF-BB compared with controls (p < 0.05). The effect of PDGF-BB gene transfer on diabetic and non-diabetic fibroblasts was similar to that of 20 ng/ml or less of PDGF-BB.

Original languageEnglish (US)
Pages (from-to)301-307
Number of pages7
JournalScandinavian Journal of Plastic and Reconstructive Surgery and Hand Surgery
Volume34
Issue number4
DOIs
StatePublished - 2000
Externally publishedYes

Fingerprint

Firearms
Collagen
Fibroblasts
Skin
Genes
platelet-derived growth factor BB
DNA
Thymidine
Wounds and Injuries

Keywords

  • Diabetes
  • Fibroblast
  • Gene gun
  • Gene transfer
  • PDGF

ASJC Scopus subject areas

  • Surgery

Cite this

Transfer of platelet-derived growth factor-BB gene by gene gun increases contraction of collagen lattice by fibroblasts in diabetic and non-diabetic human skin. / Kuwahara, H.; Mitchell, A. T.; Macklin, M. D.; Zhao, J.; Listengarten, D.; Phillips, Linda.

In: Scandinavian Journal of Plastic and Reconstructive Surgery and Hand Surgery, Vol. 34, No. 4, 2000, p. 301-307.

Research output: Contribution to journalArticle

@article{d2d0bffd4c9c4cf58804516902a6aee9,
title = "Transfer of platelet-derived growth factor-BB gene by gene gun increases contraction of collagen lattice by fibroblasts in diabetic and non-diabetic human skin",
abstract = "We have used an in vitro model of wound contraction, the fibroblast-populated collagen lattice, to examine the effect of platelet-derived growth factor BB (PDGF-BB) and PDGF-BB gene transfer by gene gun on the contraction of lattices composed of either diabetic or non-diabetic human fibroblasts. The area of collagen lattice and DNA synthesis were measured in 12 specimens. There were significant increases in lattice contraction with increasing doses of PDGF-BB and fibroblasts transfected with the PDGF-BB gene compared with control (p < 0.01). DNA synthesis of the non-diabetic and diabetic fibroblast lattices showed significantly increased incorporation of tritiated thymidine with increasing doses of PDGF-BB and fibroblasts transfected with the PDGF-BB compared with controls (p < 0.05). The effect of PDGF-BB gene transfer on diabetic and non-diabetic fibroblasts was similar to that of 20 ng/ml or less of PDGF-BB.",
keywords = "Diabetes, Fibroblast, Gene gun, Gene transfer, PDGF",
author = "H. Kuwahara and Mitchell, {A. T.} and Macklin, {M. D.} and J. Zhao and D. Listengarten and Linda Phillips",
year = "2000",
doi = "10.1080/028443100750059066",
language = "English (US)",
volume = "34",
pages = "301--307",
journal = "Journal of Plastic Surgery and Hand Surgery",
issn = "2000-656X",
publisher = "Informa Healthcare",
number = "4",

}

TY - JOUR

T1 - Transfer of platelet-derived growth factor-BB gene by gene gun increases contraction of collagen lattice by fibroblasts in diabetic and non-diabetic human skin

AU - Kuwahara, H.

AU - Mitchell, A. T.

AU - Macklin, M. D.

AU - Zhao, J.

AU - Listengarten, D.

AU - Phillips, Linda

PY - 2000

Y1 - 2000

N2 - We have used an in vitro model of wound contraction, the fibroblast-populated collagen lattice, to examine the effect of platelet-derived growth factor BB (PDGF-BB) and PDGF-BB gene transfer by gene gun on the contraction of lattices composed of either diabetic or non-diabetic human fibroblasts. The area of collagen lattice and DNA synthesis were measured in 12 specimens. There were significant increases in lattice contraction with increasing doses of PDGF-BB and fibroblasts transfected with the PDGF-BB gene compared with control (p < 0.01). DNA synthesis of the non-diabetic and diabetic fibroblast lattices showed significantly increased incorporation of tritiated thymidine with increasing doses of PDGF-BB and fibroblasts transfected with the PDGF-BB compared with controls (p < 0.05). The effect of PDGF-BB gene transfer on diabetic and non-diabetic fibroblasts was similar to that of 20 ng/ml or less of PDGF-BB.

AB - We have used an in vitro model of wound contraction, the fibroblast-populated collagen lattice, to examine the effect of platelet-derived growth factor BB (PDGF-BB) and PDGF-BB gene transfer by gene gun on the contraction of lattices composed of either diabetic or non-diabetic human fibroblasts. The area of collagen lattice and DNA synthesis were measured in 12 specimens. There were significant increases in lattice contraction with increasing doses of PDGF-BB and fibroblasts transfected with the PDGF-BB gene compared with control (p < 0.01). DNA synthesis of the non-diabetic and diabetic fibroblast lattices showed significantly increased incorporation of tritiated thymidine with increasing doses of PDGF-BB and fibroblasts transfected with the PDGF-BB compared with controls (p < 0.05). The effect of PDGF-BB gene transfer on diabetic and non-diabetic fibroblasts was similar to that of 20 ng/ml or less of PDGF-BB.

KW - Diabetes

KW - Fibroblast

KW - Gene gun

KW - Gene transfer

KW - PDGF

UR - http://www.scopus.com/inward/record.url?scp=0034537998&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=0034537998&partnerID=8YFLogxK

U2 - 10.1080/028443100750059066

DO - 10.1080/028443100750059066

M3 - Article

C2 - 11195866

AN - SCOPUS:0034537998

VL - 34

SP - 301

EP - 307

JO - Journal of Plastic Surgery and Hand Surgery

JF - Journal of Plastic Surgery and Hand Surgery

SN - 2000-656X

IS - 4

ER -