Transfer of platelet-derived growth factor-BB gene by gene gun increases contraction of collagen lattice by fibroblasts in diabetic and non-diabetic human skin

H. Kuwahara, A. T. Mitchell, M. D. Macklin, J. Zhao, D. Listengarten, L. G. Phillips

Research output: Contribution to journalArticle

4 Scopus citations

Abstract

We have used an in vitro model of wound contraction, the fibroblast-populated collagen lattice, to examine the effect of platelet-derived growth factor BB (PDGF-BB) and PDGF-BB gene transfer by gene gun on the contraction of lattices composed of either diabetic or non-diabetic human fibroblasts. The area of collagen lattice and DNA synthesis were measured in 12 specimens. There were significant increases in lattice contraction with increasing doses of PDGF-BB and fibroblasts transfected with the PDGF-BB gene compared with control (p < 0.01). DNA synthesis of the non-diabetic and diabetic fibroblast lattices showed significantly increased incorporation of tritiated thymidine with increasing doses of PDGF-BB and fibroblasts transfected with the PDGF-BB compared with controls (p < 0.05). The effect of PDGF-BB gene transfer on diabetic and non-diabetic fibroblasts was similar to that of 20 ng/ml or less of PDGF-BB.

Original languageEnglish (US)
Pages (from-to)301-307
Number of pages7
JournalScandinavian Journal of Plastic and Reconstructive Surgery and Hand Surgery
Volume34
Issue number4
DOIs
StatePublished - Dec 1 2000
Externally publishedYes

Keywords

  • Diabetes
  • Fibroblast
  • Gene gun
  • Gene transfer
  • PDGF

ASJC Scopus subject areas

  • Medicine(all)

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