Transformation of an insect symbiont and expression of a foreign gene in the Chagas' disease vector Rhodnius prolixus

C. B. Beard, P. W. Mason, S. Aksoy, R. B. Tesh, F. F. Richards

Research output: Contribution to journalArticle

73 Citations (Scopus)

Abstract

A shuttle plasmid was developed that is capable of replicating both in Escherichia coli and in Rhodococcus rhodnii, a bacterial symbiont of the Chagas' disease vector Rhodnius prolixus. We have been able to transform R. rhodnii with this plasmid, infect aposymbiotic R. prolixus with the transformed symbiont, select with the antibiotic thiostrepton, and re-isolate genetically altered symbionts from the insects following successive molts. Symbiotic bacteria are potentially valuable as vehicles for the stable introduction of foreign genes into insects with the goal of eventually altering the ability of the insect to transmit a pathogenic agent.

Original languageEnglish (US)
Pages (from-to)195-200
Number of pages6
JournalAmerican Journal of Tropical Medicine and Hygiene
Volume46
Issue number2
StatePublished - 1992
Externally publishedYes

Fingerprint

Rhodnius
Disease Vectors
Chagas Disease
Insects
Thiostrepton
Plasmids
Insect Genes
Genes
Rhodococcus
Escherichia coli
Anti-Bacterial Agents
Bacteria

ASJC Scopus subject areas

  • Parasitology
  • Infectious Diseases

Cite this

Transformation of an insect symbiont and expression of a foreign gene in the Chagas' disease vector Rhodnius prolixus. / Beard, C. B.; Mason, P. W.; Aksoy, S.; Tesh, R. B.; Richards, F. F.

In: American Journal of Tropical Medicine and Hygiene, Vol. 46, No. 2, 1992, p. 195-200.

Research output: Contribution to journalArticle

Beard, C. B. ; Mason, P. W. ; Aksoy, S. ; Tesh, R. B. ; Richards, F. F. / Transformation of an insect symbiont and expression of a foreign gene in the Chagas' disease vector Rhodnius prolixus. In: American Journal of Tropical Medicine and Hygiene. 1992 ; Vol. 46, No. 2. pp. 195-200.
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