Transformation proteins and DNA uptake localize to the cell poles in Bacillus subtilis

Jeanette Hahn, Berenike Maier, Bert Jan Haijema, Michael Sheetz, David Dubnau

Research output: Contribution to journalArticle

86 Citations (Scopus)

Abstract

The Gram-positive, rod-forming bacterium Bacillus subtilis efficiently binds and internalizes transforming DNA. The localization of several competence proteins, required for DNA uptake, has been studied using fluorescence microscopy. At least three proteins (ComGA, ComFA, and YwpH) are preferentially associated with the cell poles and appear to colocalize. This association is dynamic; the proteins accumulate at the poles as transformability develops and then delocalize as transformability wanes. DNA binding and uptake also occur preferentially at the cell poles, as shown using fluorescent DNA and in single-molecule experiments with laser tweezers. In addition to the prominent polar sites, the competence proteins also localize as foci in association with the lateral cell membrane, but this distribution does not exhibit the same temporal changes as the polar accumulation. The results suggest the regulated assembly and disassembly of a DNA-uptake machine at the cell poles.

Original languageEnglish (US)
Pages (from-to)59-71
Number of pages13
JournalCell
Volume122
Issue number1
DOIs
StatePublished - Jul 15 2005
Externally publishedYes

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Bacilli
Bacillus subtilis
Poles
DNA
Proteins
Mental Competency
Gram-Positive Rods
Optical Tweezers
Fluorescence microscopy
Cell membranes
Fluorescence Microscopy
Bacteria
Cell Membrane
Molecules
Lasers
Experiments

ASJC Scopus subject areas

  • Biochemistry, Genetics and Molecular Biology(all)

Cite this

Transformation proteins and DNA uptake localize to the cell poles in Bacillus subtilis. / Hahn, Jeanette; Maier, Berenike; Haijema, Bert Jan; Sheetz, Michael; Dubnau, David.

In: Cell, Vol. 122, No. 1, 15.07.2005, p. 59-71.

Research output: Contribution to journalArticle

Hahn, Jeanette ; Maier, Berenike ; Haijema, Bert Jan ; Sheetz, Michael ; Dubnau, David. / Transformation proteins and DNA uptake localize to the cell poles in Bacillus subtilis. In: Cell. 2005 ; Vol. 122, No. 1. pp. 59-71.
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