Transforming growth factor-β inhibits rat intestinal cell growth by regulating cell cycle specific gene expression

Tien C. Ko, R. Daniel Beauchamp, Courtney Townsend, E. Aubrey Thompson, James C. Thompson

Research output: Contribution to journalArticle

38 Citations (Scopus)

Abstract

Transforming growth factor-β1 (TGF-β1) inhibits the growth of intestinal cells, but the mechanisms involved are unknown. Using a rat intestinal crypt cell line (IEC-6), we determined the site of action in the cell cycle that TGF-β1 acts to suppress proliferation. We also examined the effect of TGF-β1 on the expression of proliferation-associated "immediate early" genes (zif268, jun-B, c-myc) during the early g1 phase and the cdc2 gene during the transition from the G1 phase to the S phase of the cell cycle. Cell cycle progression was determined by incorporation of 3H-thymidine, and gene expression was analyzed by Northern blot analysis. We found that TGF-β1 acts to inhibit proliferation of rat intestinal crypt cells by blocking cell cycle progression at the middle g1 phase. The genes activated during g1 can be divided into TGF-β1 insensitive (zif268, jun-B, and c-myc) and TGF-β1 sensitive (the cdc2 gene). TGF-β1 suppresses the induction of the cdc2 gene during the G1 S transition without inhibiting the activation of immediate early genes during the early g1 phase.

Original languageEnglish (US)
Pages (from-to)14-20
Number of pages7
JournalThe American Journal of Surgery
Volume167
Issue number1
DOIs
StatePublished - 1994

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cdc Genes
Transforming Growth Factors
Gene Expression
G1 Phase
Growth
Cell Cycle
Immediate-Early Genes
Genes
S Phase
Northern Blotting
Thymidine
Cell Line

ASJC Scopus subject areas

  • Surgery

Cite this

Transforming growth factor-β inhibits rat intestinal cell growth by regulating cell cycle specific gene expression. / Ko, Tien C.; Beauchamp, R. Daniel; Townsend, Courtney; Thompson, E. Aubrey; Thompson, James C.

In: The American Journal of Surgery, Vol. 167, No. 1, 1994, p. 14-20.

Research output: Contribution to journalArticle

Ko, Tien C. ; Beauchamp, R. Daniel ; Townsend, Courtney ; Thompson, E. Aubrey ; Thompson, James C. / Transforming growth factor-β inhibits rat intestinal cell growth by regulating cell cycle specific gene expression. In: The American Journal of Surgery. 1994 ; Vol. 167, No. 1. pp. 14-20.
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