Transgenic maize plants expressing the Totivirus antifungal protein, KP4, are highly resistant to corn smut

Aron Allen, Emir Islamovic, Jagdeep Kaur, Scott Gold, Dilip Shah, Thomas Smith

Research output: Contribution to journalArticle

22 Citations (Scopus)

Abstract

The corn smut fungus, Ustilago maydis, is a global pathogen responsible for extensive agricultural losses. Control of corn smut using traditional breeding has met with limited success because natural resistance to U. maydis is organ specific and involves numerous maize genes. Here, we present a transgenic approach by constitutively expressing the Totivirus antifungal protein KP4, in maize. Transgenic maize plants expressed high levels of KP4 with no apparent negative impact on plant development and displayed robust resistance to U. maydis challenges to both the stem and ear tissues in the greenhouse. More broadly, these results demonstrate that a high level of organ independent fungal resistance can be afforded by transgenic expression of this family of antifungal proteins.

Original languageEnglish (US)
Pages (from-to)857-864
Number of pages8
JournalPlant Biotechnology Journal
Volume9
Issue number8
DOIs
StatePublished - Oct 2011
Externally publishedYes

Fingerprint

Totivirus
antifungal proteins
Genetically Modified Plants
Zea mays
Ustilago zeae
genetically modified organisms
corn
Proteins
Ustilago
Plant Development
Innate Immunity
Breeding
Ear
plant development
ears
Fungi
greenhouses
gene expression
fungi
stems

Keywords

  • KP4
  • Transgenic
  • Ustilago maydis

ASJC Scopus subject areas

  • Plant Science
  • Biotechnology
  • Agronomy and Crop Science

Cite this

Transgenic maize plants expressing the Totivirus antifungal protein, KP4, are highly resistant to corn smut. / Allen, Aron; Islamovic, Emir; Kaur, Jagdeep; Gold, Scott; Shah, Dilip; Smith, Thomas.

In: Plant Biotechnology Journal, Vol. 9, No. 8, 10.2011, p. 857-864.

Research output: Contribution to journalArticle

Allen, Aron ; Islamovic, Emir ; Kaur, Jagdeep ; Gold, Scott ; Shah, Dilip ; Smith, Thomas. / Transgenic maize plants expressing the Totivirus antifungal protein, KP4, are highly resistant to corn smut. In: Plant Biotechnology Journal. 2011 ; Vol. 9, No. 8. pp. 857-864.
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