Transgenic mouse model for echovirus myocarditis and paralysis

Scott A. Hughes, Harshwardhan Thaker, Vincent R. Racaniello

Research output: Contribution to journalArticle

22 Citations (Scopus)

Abstract

Echoviruses have been implicated in multiple human disease syndromes, including aseptic meningitis, paralysis, and heart disease, but no animal model is available for studying the pathogenesis of infection. Production of human integrin very late antigen 2, a receptor for echovirus type 1, in transgenic mice conferred susceptibility to viral infection. Intracerebral inoculation of newborn transgenic mice with echovirus leads to paralysis and wasting. No disease was observed in infected nontransgenic mice. In paralyzed mice significant damage was observed in the outer layers of the cerebrum, and numerous condensed neuronal nuclei were present. In contrast, intracerebral inoculation of adolescent (3- to 4-week-old) transgenic mice with echovirus type 1 did not lead to paralysis but an acute wasting phenotype and myocarditis. These findings establish human very late antigen 2 transgenic mice as a model for echovirus pathogenesis.

Original languageEnglish (US)
Pages (from-to)15906-15911
Number of pages6
JournalProceedings of the National Academy of Sciences of the United States of America
Volume100
Issue number26
DOIs
StatePublished - Dec 23 2003
Externally publishedYes

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Human Enterovirus B
Myocarditis
Paralysis
Transgenic Mice
Integrin alpha2beta1
Aseptic Meningitis
Cerebrum
Virus Diseases
Integrins
Heart Diseases
Animal Models
Phenotype
Infection

ASJC Scopus subject areas

  • General

Cite this

Transgenic mouse model for echovirus myocarditis and paralysis. / Hughes, Scott A.; Thaker, Harshwardhan; Racaniello, Vincent R.

In: Proceedings of the National Academy of Sciences of the United States of America, Vol. 100, No. 26, 23.12.2003, p. 15906-15911.

Research output: Contribution to journalArticle

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