Transient or occult HIV infections may occur more frequently than progressive infections: changing the paradigm about HIV persistence.

G. K. Sahu, T. McNearney, A. Evans, A. Turner, Scott Weaver, J. C. Huang, S. Baron, D. Paar, M. W. Cloyd

Research output: Contribution to journalArticle

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Abstract

Evidence of transient HIV infections was found in 8 subjects at high-risk for HIV infection among 47 longitudinally studied over 2-5 (average approximately 3.5) years, whereas only two subjects developed progressive infection. All of these subjects developed serum antibodies (Ab) to conformational epitopes of HIV gp41 (termed "early HIV Ab"), but the 8 transiently infected subjects lost this Ab within 4-18 months, and did not seroconvert to positivity in denatured antigen EIA or Western Blot (WB). However, the two progressively infected subjects eventually seroconverted in the EIA and WB tests within one to two months after the appearance of "early HIV Ab". HIV env and nef sequences were directly PCR amplified from the peripheral blood mononuclear cells (PBMCs) of two of the eight transiently infected subjects during the time of "early HIV Ab"-postivity, and these showed significant sequence divergence from the HIV strains in the laboratory, indicating that they were not laboratory contaminants. Genome identity typing ("paternity-typing") of PBMC samples obtained at the time of "early HIV Ab"-positivity, and later when Ab was absent from each of the 8 subjects, showed that blood samples were not mixed-up. This provides further evidence that transient or occult infection with HIV does occur, and perhaps at a greater frequency than do progressive infections.

Original languageEnglish (US)
Pages (from-to)131-145
Number of pages15
JournalArchives of virology. Supplementum.
Issue number19
StatePublished - 2005

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HIV Antibodies
HIV Infections
HIV
Antibodies
Blood Cells
HIV Envelope Protein gp41
Infection
Western Blotting
HIV Seropositivity
Epitopes
Genome
Antigens
Polymerase Chain Reaction
Serum

ASJC Scopus subject areas

  • Immunology and Microbiology(all)

Cite this

Transient or occult HIV infections may occur more frequently than progressive infections : changing the paradigm about HIV persistence. / Sahu, G. K.; McNearney, T.; Evans, A.; Turner, A.; Weaver, Scott; Huang, J. C.; Baron, S.; Paar, D.; Cloyd, M. W.

In: Archives of virology. Supplementum., No. 19, 2005, p. 131-145.

Research output: Contribution to journalArticle

Sahu, G. K. ; McNearney, T. ; Evans, A. ; Turner, A. ; Weaver, Scott ; Huang, J. C. ; Baron, S. ; Paar, D. ; Cloyd, M. W. / Transient or occult HIV infections may occur more frequently than progressive infections : changing the paradigm about HIV persistence. In: Archives of virology. Supplementum. 2005 ; No. 19. pp. 131-145.
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