Transient poration and cell surface receptor removal from human lymphocytes in vitro by 1 MHZ ultrasound

Andrew A. Brayman, Myra L. Coppage, Smita Vaidya, Morton W. Miller

Research output: Contribution to journalArticle

67 Citations (Scopus)

Abstract

The study objective was to gain insight into ultrasound-induced, sub-lytic cell surface modifications. Two primary hypotheses were tested by flow cytometric methods; viz., sonication will: 1. remove all or part of a specific cell surface marker in lymphocytes surviving insonation, and 2. induce transient pores in the cell membranes of some surviving cells. RPMI 1788 human lymphocytes were exposed in vitro to 1-MHz, continuous-wave ultrasound (approx. 8 W/cm2 ISP) for 30 s, which lysed approx. 50% of the cells. Insonation: 1. altered cell morphology, increasing the population of cells of reduced size but high structure (designated as population R2), many of which were nonviable, and diminishing the population of cells of large size and high structure (designated as population R1), most of which were viable, 2. diminished the fluorescence signal from the pan B lymphocyte marker CD19 in populations R1 and R2 to equivalent extents, and 3. increased by approx. 7-fold the number of transiently permeabilized cells in R1, as evidenced by simultaneous uptake of propidium iodide and fluorescein diacetate. The results indicate that ultrasound-induced CD19 removal from R1 cells can occur without accompanying gross membrane loss. The cell morphology/mortality shifts indicate that the ultrasound-induced morphological change is associated with lethal membrane poration, suggesting that the diminished CD19 fluorescence signal from insonated R2 cells arises partly by simultaneous loss of membrane fragments, CD19 and cytoplasm.

Original languageEnglish (US)
Pages (from-to)999-1008
Number of pages10
JournalUltrasound in Medicine and Biology
Volume25
Issue number6
DOIs
StatePublished - 1999

Fingerprint

lymphocytes
Cell Surface Receptors
Lymphocytes
cells
Population
membranes
Cell Size
markers
Membranes
Fluorescence
In Vitro Techniques
fluorescence
Sonication
cytoplasm
Propidium
mortality
iodides
continuous radiation
Cytoplasm
B-Lymphocytes

Keywords

  • CD19
  • Cell surface marker
  • Inertial cavitation
  • Lymphocytes
  • Membrane poration
  • Ultrasound bioeffects

ASJC Scopus subject areas

  • Radiology Nuclear Medicine and imaging

Cite this

Transient poration and cell surface receptor removal from human lymphocytes in vitro by 1 MHZ ultrasound. / Brayman, Andrew A.; Coppage, Myra L.; Vaidya, Smita; Miller, Morton W.

In: Ultrasound in Medicine and Biology, Vol. 25, No. 6, 1999, p. 999-1008.

Research output: Contribution to journalArticle

Brayman, Andrew A. ; Coppage, Myra L. ; Vaidya, Smita ; Miller, Morton W. / Transient poration and cell surface receptor removal from human lymphocytes in vitro by 1 MHZ ultrasound. In: Ultrasound in Medicine and Biology. 1999 ; Vol. 25, No. 6. pp. 999-1008.
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