Transient receptor potential melastatin type 7 channel is critical for the survival of bone marrow derived mesenchymal stem cells

Henrique Cheng, Ji Ming Feng, Marxa L. Figueiredo, Hanjie Zhang, Piper L. Nelson, Vanessa Marigo, Andreas Beck

Research output: Contribution to journalArticle

41 Citations (Scopus)

Abstract

The transient receptor potential melastatin type 7 channel (TRPM7) is a member of the TRP family of ion channels that is essential for cell proliferation and viability. Mesenchymal stem cells (MSCs) from bone marrow are a potential source for tissue repair due to their ability to differentiate into specialized cells. However, the role of TRPM7 in stem cells is unknown. In this study, we characterized TRPM7 in mouse MSCs using molecular biology, immunocytochemistry, and patch clamp. We also investigated TRPM7 function using a lentiviral vector and specific shRNA to knockdown gene expression. By RT-PCR and immunocytochemistry, we identified TRPM7, but not TRPM6, a close family member with similar function. Electrophysiological recordings during depletion of intracellular Mg2+ or Mg2+-ATP resulted in the development of currents typical for the channel. Furthermore, 2-aminoethoxydiphenyl borate (1 pM-100μM) inhibited TRPM7 in a concentration-dependent manner. The molecular suppression of TRPM7 significantly decreased MSC proliferation and viability as determined by MTT assay. In addition, TRPM7 gene expression was up-regulated during osteogenesis. These findings demonstrate that TRPM7 is required for MSC survival and perhaps involved in the differentiation process.

Original languageEnglish (US)
Pages (from-to)1393-1403
Number of pages11
JournalStem Cells and Development
Volume19
Issue number9
DOIs
StatePublished - Sep 1 2010
Externally publishedYes

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Mesenchymal Stromal Cells
Bone Marrow
Cell Survival
Immunohistochemistry
Cell Proliferation
Gene Expression
Aptitude
Ion Channels
Osteogenesis
Small Interfering RNA
Molecular Biology
Stem Cells
Adenosine Triphosphate
Polymerase Chain Reaction

ASJC Scopus subject areas

  • Cell Biology
  • Developmental Biology
  • Hematology

Cite this

Transient receptor potential melastatin type 7 channel is critical for the survival of bone marrow derived mesenchymal stem cells. / Cheng, Henrique; Feng, Ji Ming; Figueiredo, Marxa L.; Zhang, Hanjie; Nelson, Piper L.; Marigo, Vanessa; Beck, Andreas.

In: Stem Cells and Development, Vol. 19, No. 9, 01.09.2010, p. 1393-1403.

Research output: Contribution to journalArticle

Cheng, Henrique ; Feng, Ji Ming ; Figueiredo, Marxa L. ; Zhang, Hanjie ; Nelson, Piper L. ; Marigo, Vanessa ; Beck, Andreas. / Transient receptor potential melastatin type 7 channel is critical for the survival of bone marrow derived mesenchymal stem cells. In: Stem Cells and Development. 2010 ; Vol. 19, No. 9. pp. 1393-1403.
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