Transient transcriptional events in human skeletal muscle at the outset of concentric resistance exercise training

Andrew Murton, R. Billeter, F. B. Stephens, S. G. Des Etages, F. Graber, R. J. Hill, K. Marimuthu, P. L. Greenhaff

Research output: Contribution to journalArticle

23 Citations (Scopus)

Abstract

We sought to ascertain the time course of transcriptional events that occur in human skeletal muscle at the outset of resistance exercise (RE) training in RE naive individuals and determine whether the magnitude of response was associated with exercise-induced muscle damage. Sixteen RE naive men were recruited; eight underwent two sessions of 5 × 30 maximum isokinetic knee extensions (180°/s) separated by 48 h. Muscle biopsies of the vastus lateralis, obtained from different sites, were taken at baseline and 24 h after each exercise bout. Eight individuals acted as nonexercise controls with biopsies obtained at the same time intervals. Transcriptional changes were assessed by microarray and protein levels of heat shock protein (HSP) 27 and αB-crystallin in muscle cross sections by immunohistochemistry as a proxy measure of muscle damage. In control subjects, no probe sets were significantly altered (false discovery rate < 0.05), and HSP27 and αB-crystallin protein remained unchanged throughout the study. In exercised subjects, significant intersubject variability following the initial RE bout was observed in the muscle transcriptome, with greatest changes occurring in subjects with elevated HSP27 and αB-crystallin protein. Following the second bout, the transcriptome response was more consistent, revealing a cohort of probe sets associated with immune activation, the suppression of oxidative metabolism, and ubiquitination, as differentially regulated. The results reveal that the initial transcriptional response to RE is variable in RE naive volunteers, potentially associated with muscle damage and unlikely to reflect longer term adaptations to RE training. These results highlight the importance of considering multiple time points when determining the transcriptional response to RE and associated physiological adaptation.

Original languageEnglish (US)
Pages (from-to)113-125
Number of pages13
JournalJournal of Applied Physiology
Volume116
Issue number1
DOIs
StatePublished - Jan 1 2014
Externally publishedYes

Fingerprint

Resistance Training
Skeletal Muscle
Exercise
Muscles
Crystallins
Transcriptome
HSP27 Heat-Shock Proteins
Physiological Adaptation
Biopsy
Protein Array Analysis
Ubiquitination
Quadriceps Muscle
Proxy
Volunteers
Knee
Immunohistochemistry

Keywords

  • Exercise-induced muscle damage
  • Muscle biopsies
  • Muscle transcriptome
  • Resistance exercise
  • Training adaptation

ASJC Scopus subject areas

  • Physiology
  • Physiology (medical)

Cite this

Transient transcriptional events in human skeletal muscle at the outset of concentric resistance exercise training. / Murton, Andrew; Billeter, R.; Stephens, F. B.; Des Etages, S. G.; Graber, F.; Hill, R. J.; Marimuthu, K.; Greenhaff, P. L.

In: Journal of Applied Physiology, Vol. 116, No. 1, 01.01.2014, p. 113-125.

Research output: Contribution to journalArticle

Murton, A, Billeter, R, Stephens, FB, Des Etages, SG, Graber, F, Hill, RJ, Marimuthu, K & Greenhaff, PL 2014, 'Transient transcriptional events in human skeletal muscle at the outset of concentric resistance exercise training', Journal of Applied Physiology, vol. 116, no. 1, pp. 113-125. https://doi.org/10.1152/japplphysiol.00426.2013
Murton, Andrew ; Billeter, R. ; Stephens, F. B. ; Des Etages, S. G. ; Graber, F. ; Hill, R. J. ; Marimuthu, K. ; Greenhaff, P. L. / Transient transcriptional events in human skeletal muscle at the outset of concentric resistance exercise training. In: Journal of Applied Physiology. 2014 ; Vol. 116, No. 1. pp. 113-125.
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