Translocation and survival of bactevoides fragilis after thermal injury

Luca Gianotti, J. Wesley Alexander, Tonyia Pyles, Roberto Gennari, George F. Babcock

Research output: Contribution to journalArticle

Abstract

B. fragilis and E. coli were labeled with tritiatcd (3H) thymidine, and 1010 of each were given separately by gavage in Balb/c mice immediately before a 20% burn injury was inflicted. Control groups received gavage with 3H-B. fragilis or 3H-E. coli without burn. Four hours after burn or gavage was administered, the animals were killed, and the radionuclide and colony counts were determined in the mesenteric lymph nodes, liver, and spleen. Additional groups of mice receiving gavage (B. fragilis or E. coli) and burn were observed for 10 days to study survival. The results showed that 3H-B. fragilis translocated to a greater extent than 3H-E. coli but that fewer B. fragilis than E. coli survived in tissues. Survival was 86% for animals challenged with B. fragilis versus 53% for animals challenged with E. coli. It is concluded that in this model B. fragilis translocates extensively after burn injury and that survival is closely related to the destruction of translocated bacteria. (J BURN CARE REHABIL 1995;16:127–31).

Original languageEnglish (US)
Pages (from-to)127-131
Number of pages5
JournalJournal of Burn Care and Rehabilitation
Volume16
Issue number2
DOIs
StatePublished - Jan 1 1995
Externally publishedYes

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ASJC Scopus subject areas

  • Surgery
  • Nursing(all)
  • Emergency Medicine
  • Rehabilitation
  • Health Professions(all)

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