TY - JOUR
T1 - Translocation and survival of bactevoides fragilis after thermal injury
AU - Gianotti, Luca
AU - Wesley Alexander, J.
AU - Pyles, Tonyia
AU - Gennari, Roberto
AU - Babcock, George F.
PY - 1995
Y1 - 1995
N2 - B. fragilis and E. coli were labeled with tritiatcd (3H) thymidine, and 1010 of each were given separately by gavage in Balb/c mice immediately before a 20% burn injury was inflicted. Control groups received gavage with 3H-B. fragilis or 3H-E. coli without burn. Four hours after burn or gavage was administered, the animals were killed, and the radionuclide and colony counts were determined in the mesenteric lymph nodes, liver, and spleen. Additional groups of mice receiving gavage (B. fragilis or E. coli) and burn were observed for 10 days to study survival. The results showed that 3H-B. fragilis translocated to a greater extent than 3H-E. coli but that fewer B. fragilis than E. coli survived in tissues. Survival was 86% for animals challenged with B. fragilis versus 53% for animals challenged with E. coli. It is concluded that in this model B. fragilis translocates extensively after burn injury and that survival is closely related to the destruction of translocated bacteria. (J BURN CARE REHABIL 1995;16:127–31).
AB - B. fragilis and E. coli were labeled with tritiatcd (3H) thymidine, and 1010 of each were given separately by gavage in Balb/c mice immediately before a 20% burn injury was inflicted. Control groups received gavage with 3H-B. fragilis or 3H-E. coli without burn. Four hours after burn or gavage was administered, the animals were killed, and the radionuclide and colony counts were determined in the mesenteric lymph nodes, liver, and spleen. Additional groups of mice receiving gavage (B. fragilis or E. coli) and burn were observed for 10 days to study survival. The results showed that 3H-B. fragilis translocated to a greater extent than 3H-E. coli but that fewer B. fragilis than E. coli survived in tissues. Survival was 86% for animals challenged with B. fragilis versus 53% for animals challenged with E. coli. It is concluded that in this model B. fragilis translocates extensively after burn injury and that survival is closely related to the destruction of translocated bacteria. (J BURN CARE REHABIL 1995;16:127–31).
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U2 - 10.1097/00004630-199503000-00006
DO - 10.1097/00004630-199503000-00006
M3 - Article
C2 - 7775505
AN - SCOPUS:0028932137
SN - 0273-8481
VL - 16
SP - 127
EP - 131
JO - Journal of Burn Care and Rehabilitation
JF - Journal of Burn Care and Rehabilitation
IS - 2
ER -