Transmission of Ebola virus from pigs to non-human primates

Hana M. Weingartl; Carissa Embury-Hyatt; Charles Nfon; Anders Leung; Greg Smith; Gary Kobinger

doi:10.1038/srep00811

Transmission of Ebola virus from pigs to non-human primates

Hana M. Weingartl, Carissa Embury-Hyatt, Charles Nfon, Anders Leung, Greg Smith, Gary Kobinger

Research output: Contribution to journal › Article › peer-review

143 Scopus citations

Abstract

Ebola viruses (EBOV) cause often fatal hemorrhagic fever in several species of simian primates including human. While fruit bats are considered natural reservoir, involvement of other species in EBOV transmission is unclear. In 2009, Reston-EBOV was the first EBOV detected in swine with indicated transmission to humans. In-contact transmission of Zaire-EBOV (ZEBOV) between pigs was demonstrated experimentally. Here we show ZEBOV transmission from pigs to cynomolgus macaques without direct contact. Interestingly, transmission between macaques in similar housing conditions was never observed. Piglets inoculated oro-nasally with ZEBOV were transferred to the room housing macaques in an open inaccessible cage system. All macaques became infected. Infectious virus was detected in oro-nasal swabs of piglets, and in blood, swabs, and tissues of macaques. This is the first report of experimental interspecies virus transmission, with the macaques also used as a human surrogate. Our finding may influence prevention and control measures during EBOV outbreaks.

Original language	English (US)
Article number	811
Journal	Scientific reports
Volume	2
DOIs	https://doi.org/10.1038/srep00811
State	Published - 2012
Externally published	Yes

ASJC Scopus subject areas

General

Access to Document

10.1038/srep00811

Cite this

@article{53643e8510ed400db55a252d8c50ffa4,

title = "Transmission of Ebola virus from pigs to non-human primates",

abstract = "Ebola viruses (EBOV) cause often fatal hemorrhagic fever in several species of simian primates including human. While fruit bats are considered natural reservoir, involvement of other species in EBOV transmission is unclear. In 2009, Reston-EBOV was the first EBOV detected in swine with indicated transmission to humans. In-contact transmission of Zaire-EBOV (ZEBOV) between pigs was demonstrated experimentally. Here we show ZEBOV transmission from pigs to cynomolgus macaques without direct contact. Interestingly, transmission between macaques in similar housing conditions was never observed. Piglets inoculated oro-nasally with ZEBOV were transferred to the room housing macaques in an open inaccessible cage system. All macaques became infected. Infectious virus was detected in oro-nasal swabs of piglets, and in blood, swabs, and tissues of macaques. This is the first report of experimental interspecies virus transmission, with the macaques also used as a human surrogate. Our finding may influence prevention and control measures during EBOV outbreaks.",

author = "Weingartl, {Hana M.} and Carissa Embury-Hyatt and Charles Nfon and Anders Leung and Greg Smith and Gary Kobinger",

note = "Funding Information: We would like to thank Dr. Melanie van der Loop, Kevin Tierney, and Gary Wong for the assistance with animal care, to Peter Marszal, Jill Graham and Brad Collignon for the technical assistance, and to Dr. Soren Alexandersen for the critical review of the manuscript. The project was supported by CFIA and PHAC, with funding provided from the CRTI Cluster Activity fund CRTI-3780-2011-30va-17.",

year = "2012",

doi = "10.1038/srep00811",

language = "English (US)",

volume = "2",

journal = "Scientific reports",

issn = "2045-2322",

publisher = "Nature Publishing Group",

}

TY - JOUR

T1 - Transmission of Ebola virus from pigs to non-human primates

AU - Weingartl, Hana M.

AU - Embury-Hyatt, Carissa

AU - Nfon, Charles

AU - Leung, Anders

AU - Smith, Greg

AU - Kobinger, Gary

N1 - Funding Information: We would like to thank Dr. Melanie van der Loop, Kevin Tierney, and Gary Wong for the assistance with animal care, to Peter Marszal, Jill Graham and Brad Collignon for the technical assistance, and to Dr. Soren Alexandersen for the critical review of the manuscript. The project was supported by CFIA and PHAC, with funding provided from the CRTI Cluster Activity fund CRTI-3780-2011-30va-17.

PY - 2012

Y1 - 2012

N2 - Ebola viruses (EBOV) cause often fatal hemorrhagic fever in several species of simian primates including human. While fruit bats are considered natural reservoir, involvement of other species in EBOV transmission is unclear. In 2009, Reston-EBOV was the first EBOV detected in swine with indicated transmission to humans. In-contact transmission of Zaire-EBOV (ZEBOV) between pigs was demonstrated experimentally. Here we show ZEBOV transmission from pigs to cynomolgus macaques without direct contact. Interestingly, transmission between macaques in similar housing conditions was never observed. Piglets inoculated oro-nasally with ZEBOV were transferred to the room housing macaques in an open inaccessible cage system. All macaques became infected. Infectious virus was detected in oro-nasal swabs of piglets, and in blood, swabs, and tissues of macaques. This is the first report of experimental interspecies virus transmission, with the macaques also used as a human surrogate. Our finding may influence prevention and control measures during EBOV outbreaks.

AB - Ebola viruses (EBOV) cause often fatal hemorrhagic fever in several species of simian primates including human. While fruit bats are considered natural reservoir, involvement of other species in EBOV transmission is unclear. In 2009, Reston-EBOV was the first EBOV detected in swine with indicated transmission to humans. In-contact transmission of Zaire-EBOV (ZEBOV) between pigs was demonstrated experimentally. Here we show ZEBOV transmission from pigs to cynomolgus macaques without direct contact. Interestingly, transmission between macaques in similar housing conditions was never observed. Piglets inoculated oro-nasally with ZEBOV were transferred to the room housing macaques in an open inaccessible cage system. All macaques became infected. Infectious virus was detected in oro-nasal swabs of piglets, and in blood, swabs, and tissues of macaques. This is the first report of experimental interspecies virus transmission, with the macaques also used as a human surrogate. Our finding may influence prevention and control measures during EBOV outbreaks.

UR - http://www.scopus.com/inward/record.url?scp=84870843021&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=84870843021&partnerID=8YFLogxK

U2 - 10.1038/srep00811

DO - 10.1038/srep00811

M3 - Article

C2 - 23155478

AN - SCOPUS:84870843021

SN - 2045-2322

VL - 2

JO - Scientific reports

JF - Scientific reports

M1 - 811

ER -

Transmission of Ebola virus from pigs to non-human primates

Abstract

ASJC Scopus subject areas

Access to Document

Other files and links

Fingerprint

Cite this