Transplacental transfer and metabolism of 17-α-hydroxyprogesterone caproate

Sarah J. Hemauer, Ru Yan, Svetlana L. Patrikeeva, Donald R. Mattison, Gary Hankins, Mahmoud Ahmed, Tatiana Nanovskaya

Research output: Contribution to journalArticle

19 Citations (Scopus)

Abstract

Objective: Determine transplacental transfer and metabolism of 17-α-hydroxyprogesterone caproate and its distribution between the tissue and the maternal and fetal circuits of the dually perfused placental lobule. Study Design: 17-α-Hydroxyprogesterone caproate (21 ng/mL) and its dual-labeled isotope, 17-α-hydroxy-[3H] progesterone [14C] caproate were added to the maternal circuit. The concentrations of the drug and its metabolite in trophoblast tissue and both circuits were determined by high performance liquid chromatography and liquid scintillation spectrometry. Results: 17-α-Hydroxyprogesterone caproate was transferred from the maternal to fetal circuit. After a 4-hour perfusion period, a metabolite of 17-α-hydroxyprogesterone caproate that retained both progesterone and caproate moieties was identified in the tissue and the maternal and fetal circuits. Neither 17-α-hydroxyprogesterone caproate nor its metabolite, at the concentrations tested, had adverse effect on determined viability and functional parameters of placental tissue. Conclusion: 17-α-Hydroxyprogesterone caproate was metabolized by term placental lobule during its perfusion and both parent compound and its metabolite(s) transferred to the fetal circuit.

Original languageEnglish (US)
JournalAmerican Journal of Obstetrics and Gynecology
Volume199
Issue number2
DOIs
StatePublished - Aug 2008

Fingerprint

17-alpha-Hydroxyprogesterone
Mothers
Progesterone
Fetus
Perfusion
Trophoblasts
Tissue Distribution
Isotopes
17-alpha-hydroxy-progesterone caproate
Spectrum Analysis
High Pressure Liquid Chromatography
Pharmaceutical Preparations

Keywords

  • 17-α-hydroxyprogesterone caproate
  • dual perfusion
  • metabolism
  • preterm delivery

ASJC Scopus subject areas

  • Medicine(all)
  • Obstetrics and Gynecology

Cite this

Transplacental transfer and metabolism of 17-α-hydroxyprogesterone caproate. / Hemauer, Sarah J.; Yan, Ru; Patrikeeva, Svetlana L.; Mattison, Donald R.; Hankins, Gary; Ahmed, Mahmoud; Nanovskaya, Tatiana.

In: American Journal of Obstetrics and Gynecology, Vol. 199, No. 2, 08.2008.

Research output: Contribution to journalArticle

Hemauer, Sarah J. ; Yan, Ru ; Patrikeeva, Svetlana L. ; Mattison, Donald R. ; Hankins, Gary ; Ahmed, Mahmoud ; Nanovskaya, Tatiana. / Transplacental transfer and metabolism of 17-α-hydroxyprogesterone caproate. In: American Journal of Obstetrics and Gynecology. 2008 ; Vol. 199, No. 2.
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