Transplacental transfer and metabolism of buprenorphine in preterm human placenta

Valentina Fokina, Svetlana L. Patrikeeva, Olga L. Zharikova, Tatiana Nanovskaya, Gary Hankins, Mahmoud Ahmed

Research output: Contribution to journalArticle

16 Citations (Scopus)

Abstract

We sought to determine whether gestational age affects the transplacental transfer and metabolism of buprenorphine (BUP). Transfer of BUP (10 ng/mL) and its [3H]-isotope was determined across placentas of 30 to 34 weeks of gestation utilizing the technique of dual perfusion of placental lobule. Concentration of the drug in trophoblast tissue and in maternal and fetal circuits was determined by liquid scintillation spectrometry. Microsomes prepared from placentas of 17 to 37 weeks of gestation were divided into three groups: late second, early third, and late third trimesters. Antibodies raised against human cytochrome P450 (CYP) isoforms were utilized to identify the enzyme(s) catalyzing BUP biotransformation by preterm placental microsomes. The amount of norbuprenorphine formed was determined by liquid chromatography-mass spectrometry (LC-MS). BUP transfer across the placentas of 30 to 34 weeks of gestation was similar to those at term. CYP19 antibodies caused 60% inhibition of BUP metabolism by microsomes of late second and early third trimesters and 85% by microsomes of late third trimester. The developmental changes occurring in human placenta between 30 weeks of gestation through term do not affect the transfer of BUP across human placenta. CYP19 is the major enzyme responsible for biotransformation of BUP beginning at 17 weeks of gestation until term.

Original languageEnglish (US)
Pages (from-to)25-32
Number of pages8
JournalAmerican Journal of Perinatology
Volume28
Issue number1
DOIs
StatePublished - 2011

Fingerprint

Buprenorphine
Placenta
Microsomes
Third Pregnancy Trimester
Pregnancy
Aromatase
Biotransformation
Antibodies
Trophoblasts
Enzymes
Liquid Chromatography
Isotopes
Cytochrome P-450 Enzyme System
Gestational Age
Mass Spectrometry
Spectrum Analysis
Protein Isoforms
Perfusion
Mothers
Pharmaceutical Preparations

Keywords

  • Buprenorphine
  • metabolism
  • pregnant addict
  • preterm
  • transplacental transfer

ASJC Scopus subject areas

  • Pediatrics, Perinatology, and Child Health
  • Obstetrics and Gynecology

Cite this

Transplacental transfer and metabolism of buprenorphine in preterm human placenta. / Fokina, Valentina; Patrikeeva, Svetlana L.; Zharikova, Olga L.; Nanovskaya, Tatiana; Hankins, Gary; Ahmed, Mahmoud.

In: American Journal of Perinatology, Vol. 28, No. 1, 2011, p. 25-32.

Research output: Contribution to journalArticle

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