Transport of digoxin-loaded polymeric nanoparticles across BeWo cells, an in vitro model of human placental trophoblast

Norah A. Albekairi, Sanaalarab Al-Enazy, Shariq Ali, Erik Rytting

    Research output: Contribution to journalArticle

    9 Citations (Scopus)

    Abstract

    Background: Fetal arrhythmias can lead to fetal congestive heart failure and hydrops fetalis. Digoxin (the first-line treatment) has low transplacental permeability and high risk of maternal side effects. Biodegradable digoxin-loaded PEGylated poly(lactic-co-glycolic acid) nanoparticles may increase digoxin transport across BeWo b30 cell monolayers (an in vitro model of trophoblast in human placenta) by reducing the drug's interaction with P-gp. Results/methodology: The nanoparticles showed high encapsulation efficiency and sustained release over 48 h. Transport studies revealed significantly increased permeability across BeWo cell layers of digoxin-loaded nanoparticles when compared with free digoxin. P-gp inhibition also increased the permeability of digoxin, but not digoxin-loaded nanoparticles. Conclusion: This represents a novel treatment strategy for fetal cardiovascular disease which may improve maternal and fetal outcomes.

    Original languageEnglish (US)
    Pages (from-to)1325-1334
    Number of pages10
    JournalTherapeutic Delivery
    Volume6
    Issue number12
    DOIs
    StatePublished - Dec 1 2015

    Fingerprint

    Digoxin
    Trophoblasts
    Nanoparticles
    Permeability
    Fetal Diseases
    Hydrops Fetalis
    Fetal Heart
    In Vitro Techniques
    Drug Interactions
    Placenta
    Cardiac Arrhythmias
    Cardiovascular Diseases
    Heart Failure
    Mothers

    ASJC Scopus subject areas

    • Pharmaceutical Science

    Cite this

    Transport of digoxin-loaded polymeric nanoparticles across BeWo cells, an in vitro model of human placental trophoblast. / Albekairi, Norah A.; Al-Enazy, Sanaalarab; Ali, Shariq; Rytting, Erik.

    In: Therapeutic Delivery, Vol. 6, No. 12, 01.12.2015, p. 1325-1334.

    Research output: Contribution to journalArticle

    Albekairi, Norah A. ; Al-Enazy, Sanaalarab ; Ali, Shariq ; Rytting, Erik. / Transport of digoxin-loaded polymeric nanoparticles across BeWo cells, an in vitro model of human placental trophoblast. In: Therapeutic Delivery. 2015 ; Vol. 6, No. 12. pp. 1325-1334.
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    AU - Rytting, Erik

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