Transport of digoxin-loaded polymeric nanoparticles across BeWo cells, an in vitro model of human placental trophoblast

Norah A. Albekairi, Sanaalarab Al-Enazy, Shariq Ali, Erik Rytting

    Research output: Contribution to journalArticle

    11 Scopus citations

    Abstract

    Background: Fetal arrhythmias can lead to fetal congestive heart failure and hydrops fetalis. Digoxin (the first-line treatment) has low transplacental permeability and high risk of maternal side effects. Biodegradable digoxin-loaded PEGylated poly(lactic-co-glycolic acid) nanoparticles may increase digoxin transport across BeWo b30 cell monolayers (an in vitro model of trophoblast in human placenta) by reducing the drug's interaction with P-gp. Results/methodology: The nanoparticles showed high encapsulation efficiency and sustained release over 48 h. Transport studies revealed significantly increased permeability across BeWo cell layers of digoxin-loaded nanoparticles when compared with free digoxin. P-gp inhibition also increased the permeability of digoxin, but not digoxin-loaded nanoparticles. Conclusion: This represents a novel treatment strategy for fetal cardiovascular disease which may improve maternal and fetal outcomes.

    Original languageEnglish (US)
    Pages (from-to)1325-1334
    Number of pages10
    JournalTherapeutic Delivery
    Volume6
    Issue number12
    DOIs
    StatePublished - Dec 1 2015

    ASJC Scopus subject areas

    • Pharmaceutical Science

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