Treatment of HeLa cells with bacterial water extracts inhibits Shigella flexneri invasion

Bonny Breckinridge DiNovo, Richard Doan, Roy B. Dyer, Samuel Baron, Norbert K. Herzog, David W. Niesel

Research output: Contribution to journalArticle

3 Scopus citations

Abstract

Pathogenesis mediated by Shigella flexneri requires invasion of the gastrointestinal epithelium. It has been previously shown that HeLa cells challenged with S. flexneri show alterations in their phosphotyrosine-containing protein profile. In this report, we demonstrated that bacterial water extracts (WE) abrogated the invasion of HeLa cells by S. flexneri in a dose-dependent manner. A proteinaceous component of S. flexneri was shown to be responsible for this inhibitory activity. Proteins encoded on the 140-MDa plasmid were not responsible for the observed inhibition. WE from other Gram-negative bacteria also inhibited Shigella invasion of HeLa cells. HeLa cells pretreated with WE showed changes in the profile and the intensity of phosphotyrosine-containing protein bands. These data were consistent with a surface protein component in WE which initiated aberrant host cell signaling at the membrane which may account for the inhibition of bacterial entry.

Original languageEnglish (US)
Pages (from-to)149-158
Number of pages10
JournalFEMS Immunology and Medical Microbiology
Volume15
Issue number2-3
DOIs
StatePublished - Sep 1996

Keywords

  • Cell signaling
  • Cellular invasion
  • Shigella flexneri

ASJC Scopus subject areas

  • Immunology and Allergy
  • Microbiology
  • Immunology
  • Microbiology (medical)
  • Infectious Diseases

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