Treatment of HeLa cells with bacterial water extracts inhibits Shigella flexneri invasion

Bonny Breckinridge DiNovo, Richard Doan, Roy B. Dyer, Samuel Baron, Norbert K. Herzog, David W. Niesel

Research output: Contribution to journalArticlepeer-review

3 Scopus citations


Pathogenesis mediated by Shigella flexneri requires invasion of the gastrointestinal epithelium. It has been previously shown that HeLa cells challenged with S. flexneri show alterations in their phosphotyrosine-containing protein profile. In this report, we demonstrated that bacterial water extracts (WE) abrogated the invasion of HeLa cells by S. flexneri in a dose-dependent manner. A proteinaceous component of S. flexneri was shown to be responsible for this inhibitory activity. Proteins encoded on the 140-MDa plasmid were not responsible for the observed inhibition. WE from other Gram-negative bacteria also inhibited Shigella invasion of HeLa cells. HeLa cells pretreated with WE showed changes in the profile and the intensity of phosphotyrosine-containing protein bands. These data were consistent with a surface protein component in WE which initiated aberrant host cell signaling at the membrane which may account for the inhibition of bacterial entry.

Original languageEnglish (US)
Pages (from-to)149-158
Number of pages10
JournalFEMS Immunology and Medical Microbiology
Issue number2-3
StatePublished - Sep 1996


  • Cell signaling
  • Cellular invasion
  • Shigella flexneri

ASJC Scopus subject areas

  • Immunology and Allergy
  • Microbiology
  • Immunology
  • Microbiology (medical)
  • Infectious Diseases


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